Spliceosomal pre-mRNA splicing: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1126 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 427 S. Ill., graph. Darst. 26 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a Spliceosomal pre-mRNA splicing |b methods and protocols |c ed. by Klemens J. Hertel |
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Datensatz im Suchindex
| _version_ | 1804152004156588032 |
|---|---|
| adam_text | Titel: Spliceosomal pre-mRNA splicing
Autor: Hertel, Klemens J
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... ix
Part I Introductory Chapters
1 The Pre-mRNA Splicing Reaction.................................. 3
Somsakul Pop Wongpalee and Shalini Sharma
2 Diversity and Evolution of Spliceosomal Systems....................... 13
Scott William Roy and Manuel Irimia
3 Mechanisms of Spliceosomal Assembly.............................. 35
Ni-ting Chiou and Kristen W. Lynch
4 Alternative Pre-mRNA Splicing.................................... 45
Stacey D. Wagner and J. Andrew Berglund
5 Regulation of Alternative Pre-mRNA Splicing......................... 55
Miguel B. Coelho and Christopher W.J. Smith
6 Introduction to (^transcriptional RNA Splicing....................... 83
Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson
7 Chromatin and Splicing......................................... 97
Nazmul Haque and Shalini Oberdoerffer
Part II Methods Chapters
8 Preparation of Splicing Competent Nuclear Extracts.................... 117
Chiu-Ho T. Webb and Klemens J. Hertel
9 Preparation of Yeast Whole Cell Splicing Extract....................... 123
Elizabeth A. Dunn and Stephen D. Rader
10 Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase........... 137
Martha R. Stark and Stephen D. Rader
11 In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract....... 151
Maliheh Movassat, William F. Mueller, and Klemens J. Hertel
12 Kinetic Analysis of In Vitro Pre-mRNA Splicing
in HeLa Nuclear Extract......................................... 161
William F. Mueller and Klemens J. Hertel
13 In Vitro Systems for Coupling RNAP II Transcription
to Splicing and Polyadenylation.................................... 169
Eric G. Folco and Robin Reed
vii
viii Contents
14 Isolation and Accumulation of Spliceosomal Assembly Intermediates........ 179
Janine O. Ilagan and Melissa S. Jurica
15 Complementation of U4 snRNA in S. cerevisiae Splicing Extracts
for Biochemical Studies of snRNP Assembly and Function............... 193
Martha R. Stark and Stephen D. Rader
16 Expression and Purification of Splicing Proteins
from Mammalian Cells.......................................... 205
Eric Allemand and Michelle L. Hastings
17 Single Molecule Approaches for Studying Spliceosome
Assembly and Catalysis.......................................... 217
Eric G. Anderson and Aaron A. Hoskins
18 Cell-Based Splicing of Minigenes................................... 243
Sarah A. Smith and Kristen W. Lynch
19 Quantifying the Ratio of Spliceosome Components Assembled
on Pre-mRNA................................................ 257
Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal
20 Antisense Methods to Modulate Pre-mRNA Splicing................... 271
Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh
21 Using Yeast Genetics to Study Splicing Mechanisms.................... 285
Munshi Azad Hossain and Tracy L. Johnson
22 Medium Throughput Analysis of Alternative Splicing
by Fluorescently Labeled R.T-PCR.................................. 299
Ryan Percifield, Daniel Murphy, and Peter Stoilov
23 Chromatin Immunoprecipitation Approaches
to Determine Co-transcriptional Nature of Splicing..................... 315
Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer
24 Computational Approaches to Mine Publicly Available Databases.......... 325
Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund
25 Approaches to Link RNA Secondary Structures
with Splicing Regulation......................................... 341
Mireya Plass and Eduardo Eyras
26 Methods to Study Splicing from High-Throughput
RNA Sequencing Data.......................................... 357
Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras
27 Global Protein-RNA Interaction Mapping at Single Nucleotide
Resolution by iCLIP-Seq........................................ 399
Chengguo Yao, Lingjie Weng, and Yongsheng Shi
28 Predicting Alternative Splicing.................................... 411
Yoseph Barash and Jorge Vaquero Garcia
Index............................................................... 425
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| indexdate | 2024-07-10T01:03:49Z |
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| language | English |
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| physical | XI, 427 S. Ill., graph. Darst. 26 cm |
| publishDate | 2014 |
| publishDateSearch | 2014 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Spliceosomal pre-mRNA splicing methods and protocols ed. by Klemens J. Hertel New York, NY [u.a.] Humana Press 2014 XI, 427 S. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1126 Springer protocols Hertel, Klemens J. Sonstige oth Methods in molecular biology 1126 (DE-604)BV035362695 1126 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027171276&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Spliceosomal pre-mRNA splicing methods and protocols Methods in molecular biology |
| title | Spliceosomal pre-mRNA splicing methods and protocols |
| title_auth | Spliceosomal pre-mRNA splicing methods and protocols |
| title_exact_search | Spliceosomal pre-mRNA splicing methods and protocols |
| title_full | Spliceosomal pre-mRNA splicing methods and protocols ed. by Klemens J. Hertel |
| title_fullStr | Spliceosomal pre-mRNA splicing methods and protocols ed. by Klemens J. Hertel |
| title_full_unstemmed | Spliceosomal pre-mRNA splicing methods and protocols ed. by Klemens J. Hertel |
| title_short | Spliceosomal pre-mRNA splicing |
| title_sort | spliceosomal pre mrna splicing methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027171276&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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