CDK9 links RNA polymerase II transcription to processing of ribosomal RNA:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-167037 Inhaltsverzeichnis |
| Beschreibung: | 113 S. Ill., graph. Darst. |
Internformat
MARC
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| 003 | DE-604 | ||
| 005 | 20140428 | ||
| 007 | t | ||
| 008 | 140306s2012 ad|| m||| 00||| eng d | ||
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| 100 | 1 | |a Burger, Kaspar |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a CDK9 links RNA polymerase II transcription to processing of ribosomal RNA |c Kaspar Burger |
| 264 | 1 | |c 2012 | |
| 300 | |a 113 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2013 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 856 | 4 | 1 | |u http://edoc.ub.uni-muenchen.de/16703/ |x Verlag |z kostenfrei |3 Volltext |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-027168085 | ||
Datensatz im Suchindex
| _version_ | 1804151999212552192 |
|---|---|
| adam_text | CONTENT
1. INTRODUCTION 10
1.0 OPENING REMARK ........ 10
1.1 THE CELL CYCLE - BASIC PRINCIPLES AND REGULATION .... 10
1.2 HIE NUCLEOLUS IS EMBEDDED IN THE CELL CYCLE..... 13
1.2.1 THE NUCLEOLUS
PROVIDES A STRUCTURAL FRAME
FORRIBOSOME BIOGENESIS 13
1.2.2 THE NUCLEOLAR STRUCTURE
IS MODULATED BY THE
CELL CYCLE ... 14
1.2.3 THE NUCLEOLUS
INFLUENCES CELL CYCLE PROGRESSION
.... 15
1.3 RIBOSOME BIOGENESIS AT THE MOLECULAR LEVEL..... 16
1.3.1 RNAPI TRANSCRIPTION ....... 16
1.3.2 RRNA PROCESSING AND MATURATION ..... 17
1.3.3 THE MULTIFUNCTIONAL NUCLEOLUS
...... 20
1.3.4 SURVEILLANCE OF RIBOSOME BIOGENESIS ..... 20
1.4 RNAPII TRANSCRIPTION ....... 21
13 SNORNA BIOGENESIS AND PRE-MRNA SPLICING ARE COUPLED EVENTS . 24
1.6 RIBOSOME BIOGENESIS IN CONTEXT OF CANCER ..... 24
1.6.1 DEREGULATION OF RNAP I TRANSCRIPTION
IN CANCER
.... 27
1.6.2 DEREGULATION OF RRNA PROCESSING AND PROTEIN SYNTHESIS
IN CANCER 27
1.7 THE NUCLEOLUS AS STRESS SENSOR ...... 28
1.8 THE CDK-INHIBITOR FLAVOPIRIDOL IMPAIRS RIBOSOME BIOGENESIS 29
1.9 SCOPE OF THIS THESIS (AUFGABENSTELLUNG) ..... 30
2. RESULTS 32
2.1 47S RRNA PROCESSING REQUIRES CDK9...... 32
2.1.1 THE NUCLEOLAR ISOFORM
OF CDK9 HAS NO IMPACT
ON RRNA PROCESSING 35
2.1.2 CDK9-MEDIATED RRNA PROCESSING IS CONSERVED IN YEAST
... 35
2.2 DEFECTIVE 47S RRNA PROCESSING FEEDS BACK ON RNAP I TRANSCRIPTION 36
2.3 CDK9 IS REQUIRED FOR CORRECT 3 ETS PROCESSING .... 38
2.4 PROCESSING OF 47S RRNA IS DEPENDENT ON RNAP II TRANSCRIPTION 40
HTTP://D-NB.INFO/1050187636
2.4.1 INHIBITION OF TRANSLATION DOES NOT INDUCE
DEFECTIVE 3 ETS PROCESSING
2.4.2 DICERL KNOCKOUT DOES NOT
INHIBIT 47S RRNA PROCESSING
.
2.4.3 DOWNREGULATION OF SNORNAS AFTER CDK9
INACTIVATION
2.5 CDK9 INACTIVATION TRIGGERS A NUCLEOLAR STRESS PHENOTYPE
2.6 APPLICATION OF 4-THIOURIDINE IN RRNA LABELLING ....
2.6.1 45U INHIBITS RRNA
SYNTHESIS ......
2.6.2 4SLL TRIGGERS NUCLEOLAR
STRESS ......
3. DISCUSSION
3.1 KINASES AT THE INTERFACE BETWEEN RNAPII TRANSCRIPTION AND RRNA
PROCESSING
3.2 ADAPTION OF RNAP I TRANSCRIPTION TO 47S RRNA PROCESSING
3.3 SNORNAS ARE RATE LIMITING FOR 47S RRNA PROCESSING
3.4 A MODEL FOR THE REGULATION OF RIBOSOME BIOGENESIS BY CDK9
3.5 RNAP IICTD IS REQUIRED FOR SNORNA BIOGENESIS ....
3.6 DIFFERENTIAL REGULATION OF RRNA SYNTHESIS BY CTKL AND BURL / 2
3.7 DEFECTIVE RRNA PROCESSING TRIGGERS NUCLEOLAR STRESS
3.8 DEFECTS IN RIBOSOME BIOGENESIS ARE THE ORIGIN OF MULTIPLE DISEASES.
3.9 OUTLOOK .........
4. MATERIALS
4.1 REAGENTS .........
4.2 CONSUMABLES AND KITS .......
4.3 EQUIPMENT .........
4.4 SOFTWARE .........
4.5 BUFFERS AND SOLUTIONS
........
4.6 MEDIA
..........
4.7 CELL LINES .........
4.8 YEAST STRAINS
.........
4.9 OLIGONUCLEOTIDES ........
4.10 PROBES..........
4.11 PRIMERS .........
4.12 ANTIBODIES .........
5. METHODS
41
43
43
46
47
48
51
52
52
54
55
57
60
62
64
65
68
70
70
71
72
73
73
75
76
76
76
77
78
78
80
5.1 CELL CULTURE
5.2 CELL MANIPULATION
80
81
82
83
85
87
89
110
110
111
112
112
113
YEAST CULTURE .
DNA ANALYTICS
RNA ANALYTICS
PROTEIN ANALYTICS
BIBLIOGRAPHY
APPENDIX
A CURRICULUM VITAE
B PUBLICATIONS
C ORAL PRESENTATIONS AND WORKSHOPS .
D POSTER PRESENTATIONS .
E ACKNOWLEDGEMENTS .
|
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| indexdate | 2024-07-10T01:03:44Z |
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| language | English |
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| physical | 113 S. Ill., graph. Darst. |
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| spelling | Burger, Kaspar Verfasser aut CDK9 links RNA polymerase II transcription to processing of ribosomal RNA Kaspar Burger 2012 113 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-167037 http://edoc.ub.uni-muenchen.de/16703/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-167037 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027168085&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Burger, Kaspar CDK9 links RNA polymerase II transcription to processing of ribosomal RNA |
| subject_GND | (DE-588)4113937-9 |
| title | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA |
| title_auth | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA |
| title_exact_search | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA |
| title_full | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA Kaspar Burger |
| title_fullStr | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA Kaspar Burger |
| title_full_unstemmed | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA Kaspar Burger |
| title_short | CDK9 links RNA polymerase II transcription to processing of ribosomal RNA |
| title_sort | cdk9 links rna polymerase ii transcription to processing of ribosomal rna |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/16703/ https://nbn-resolving.org/urn:nbn:de:bvb:19-167037 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027168085&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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