Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch und Buch Verl.
2013
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Studien zum Einfluss des Probiotikums Enterococcus faecium NCIMB 10415 und des Spurenelements Zink auf Leistung und diverse verdauungsphysiologische Parameter beim Ferkel |
| Beschreibung: | XI, 138 S. graph. Darst. |
| ISBN: | 9783863874193 |
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| 245 | 1 | 0 | |a Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets |c vorgelegt von Lena Martin |
| 246 | 1 | 3 | |a Studien zum Einfluss des Probiotikums Enterococcus faecium NCIMB 10415 und des Spurenelements Zink auf Leistung und diverse verdauungsphysiologische Parameter beim Ferkel |
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| 500 | |a Nebentitel: Studien zum Einfluss des Probiotikums Enterococcus faecium NCIMB 10415 und des Spurenelements Zink auf Leistung und diverse verdauungsphysiologische Parameter beim Ferkel | ||
| 502 | |a Berlin, Freie Univ., Diss., 2013 | ||
| 650 | 7 | |a probiotics |2 cabt | |
| 650 | 7 | |a Enterococcus faecium |2 cabt | |
| 650 | 7 | |a pigs |2 cabt | |
| 650 | 7 | |a weaning |2 cabt | |
| 650 | 7 | |a zinc oxide |2 cabt | |
| 650 | 7 | |a digestive system |2 cabt | |
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| 650 | 7 | |a reverse transcriptase PCR |2 cabt | |
| 650 | 7 | |a gene expression |2 cabt | |
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENTS V
CONTENTS
LIST OF TABLES VIII
LIST OF FIGURES IX
LIST OF ABBREVIATIONS X
CHAPTER 1: GENERAL INTRODUCTION 1
CHAPTER 2: LITERATURE REVIEW 4
2.1 DEVELOPMENT OF DIGESTIVE PHYSIOLOGY IN THE GIT OF PIGLETS 4
2.2 INFLUENCE OF WEANING ON PHYSIOLOGICAL PROCESSES AT THE INTESTINAL
WALL 5
2.2.1 MORPHOLOGICAL CHANGES 5
2.2.2 EFFECTS OF WEANING ON CELL TURNOVER AND ITS RESPECTIVE GENE
EXPRESSION 6
2.2.3 EFFECTS OF WEANING ON DIGESTIVE ENZYME ACTIVITIES AND GENE
EXPRESSION 6
2.2.4 IMPACT OF THE INTESTINAL MICROBIOTA ON GUT PHYSIOLOGY 8
2.3 FEED ADDITIVES AND NUTRITIONAL STRATEGIES DURING THE WEANING PERIOD
9
2.3.1 PROBIOTICS: LITERATURE OVERVIEW 9
2.3.1.1 APPLICATION OF PROBIOTICS AIMING AT IMPROVED GUT AND OVERALL
HEALTH IN PIGS 10
2.3.1.2 EFFECTS AND POSSIBLE MODES OF ACTION OF PROBIOTICS ON THE
COMPOSITION OF INTESTINAL MICROBIOTA IN THE PIG 11
2.3.1.3 SPECIAL APPLICATION OF ENTEROCOCCUS FAECIUM NCIMB 10415 AS
PROBIOTIC STRAIN IN PIGS 13
2.3.2 ZINC: LITERATURE OVERVIEW 15
2.3.2.1 HISTORY OF ZINC IN AGRICULTURE AND LIVESTOCK PRODUCTION 15
2.3.2.2 ZINC METABOLISM: ZINC DISTRIBUTION AND FUNCTION UNDER PHYSIO
LOGICAL SUPPLY 15
2.3.2.3 ZINC ABSORPTION AND INTRACELLULAR BINDING AND DISTRIBUTION ....
16
2.3.2.4 ZINC INTERACTIONS WITH OTHER DIETARY FACTORS 18
2.3.2.5 ZINC BIOAVAILABILITY OF DIFFERENT ZINC SOURCES 18
2.3.2.6 ZINC DEFICIENCY 22
2.3.2.7 ZINC INTOXICATION 23
2.3.2.8 ZINC REQUIREMENTS AND ZINC RECOMMENDATIONS 23
2.3.2.9 SUPPLY OF VERY HIGH DIETARY LEVELS OF ZINC IN PIGLET NUTRITION .
. 24
CHAPTER 3: AIMS AND OBJECTIVES OF THE THESIS 30
CHAPTER 4: INFLUENCE OF AGE AND ENTEROCOCCUS FAECIUM NCIMB 10415 ON THE
DEVELOPMENT OF SMALL INTESTINAL DIGESTIVE PHYSIOLOGY IN PIGLETS. 33
4.1 INTRODUCTION 35
4.2 MATERIAL AND METHODS 35
4.2.1 ANIMALS AND HOUSING 35
4.2.2 DIETS 36
4.2.3 SAMPLING AND TISSUE PREPARATION 36
4.2.4 PREPARATION OF BRUSH BORDER MEMBRANES 37
4.2.5 CHEMICAL ANALYSIS 37
HTTP://D-NB.INFO/1048737993
VI CONTENTS
4.2.6 JEJUNAL MORPHOLOGY 38
4.2.7 RNA EXTRACTION AND GENE EXPRESSION 38
4.2.8 DETERMINATION OF ENZYME ACTIVITY IN BRUSH BORDER MEMBRANES 39
4.2.9 STATISTICAL ANALYSIS 39
4.3 RESULTS 40
4.3.1 ANIMAL PERFORMANCE, HEALTH, AND APPARENT ILEAL AND TOTAL TRACT
NUTRIENT
DIGESTIBILITY 40
4.3.2 JEJUNAL MORPHOLOGY AND ENTEROCYTE REPLACEMENT 40
4.3.3 BRUSH BORDER ENZYMES 41
4.4 DISCUSSION 41
4.5 CONCLUSION 43
CHAPTER 5: PERFORMANCE, ORGAN ZINC CONCENTRATION, JEJUNAL BRUSH BORDER
MEMBRANE ENZYME ACTIVITIES AND MRNA EXPRESSION IN PIGLETS FED DIFFERENT
LEVELS OF DIETARY ZINC. 53
5.1 INTRODUCTION 55
5.2 MATERIAL AND METHODS 56
5.2.1 ANIMALS AND HOUSING 56
5.2.2 DIETS 56
5.2.3 SAMPLING AND TISSUE PREPARATION 56
5.2.4 DETERMINATION OF ORGAN ZINC CONCENTRATION 58
5.2.5 PREPARATION OF BRUSH BORDER MEMBRANES AND DETERMINATION OF ENZYME
ACTIVITIES 58
5.2.6 RNA EXTRACTION AND DETERMINATION OF GENE EXPRESSION 59
5.2.7 STATISTICAL ANALYSIS 59
5.3 RESULTS 60
5.3.1 ANIMAL PERFORMANCE AND HEALTH 60
5.3.2 ZINC CONCENTRATION IN DIFFERENT ORGANS 60
5.3.3 ENZYME ACTIVITIES OF BRUSH BORDER MEMBRANE AND INTESTINAL GENE
EXPRESSION 61
5.4 DISCUSSION 63
5.5 CONCLUSION 67
CHAPTER 6: A HIGH AMOUNT OF DIETARY ZINC CHANGES THE EXPRESSION OF ZINC
TRANSPORTERS AND METALLOTHIONEIN IN JEJUNAL EPITHELIAL CELLS IN VITRO
AND IN VIVO,
BUT DOES NOT PREVENT ZINC ACCUMULATION IN JEJUNAL TISSUE OF PIGLETS. 71
6.1 INTRODUCTION 73
6.2 MATERIAL AND METHODS 74
6.2.1 ANIMALS, DIETS AND SAMPLING 74
6.2.2 CHEMICAL ANALYSES 74
6.2.3 RESPONSE OF IPEC-J2 CELLS TO VARYING ZINC CONCENTRATION 75
6.2.4 GENE EXPRESSION 75
6.2.5 MT PROTEIN ABUNDANCE 76
6.2.6 STATISTICAL ANALYSIS 76
6.3 RESULTS 77
6.4 DISCUSSION 77
CHAPTER 7: GENERAL DISCUSSION AND CONCLUSIONS 91
CONTENTS
VII
CHAPTER 8: SUMMARY/ZUSAMMENFASSUNG 106
REFERENCES 113
PUBLICATION LIST 133
OANKSAGUNG 136
ELDESSTATTLICHE ERKLARUNG 138
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| spelling | Martin, Lena Verfasser (DE-588)1048033376 aut Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets vorgelegt von Lena Martin Studien zum Einfluss des Probiotikums Enterococcus faecium NCIMB 10415 und des Spurenelements Zink auf Leistung und diverse verdauungsphysiologische Parameter beim Ferkel Berlin Mensch und Buch Verl. 2013 XI, 138 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Studien zum Einfluss des Probiotikums Enterococcus faecium NCIMB 10415 und des Spurenelements Zink auf Leistung und diverse verdauungsphysiologische Parameter beim Ferkel Berlin, Freie Univ., Diss., 2013 probiotics cabt Enterococcus faecium cabt pigs cabt weaning cabt zinc oxide cabt digestive system cabt piglets cabt jejunum cabt enzymes cabt reverse transcriptase PCR cabt gene expression cabt Futterzusatz (DE-588)4155774-8 gnd rswk-swf Zink (DE-588)4137453-8 gnd rswk-swf Dünndarm (DE-588)4013218-3 gnd rswk-swf Probiotikum (DE-588)4338112-1 gnd rswk-swf Ferkel (DE-588)4154007-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ferkel (DE-588)4154007-4 s Dünndarm (DE-588)4013218-3 s Futterzusatz (DE-588)4155774-8 s Probiotikum (DE-588)4338112-1 s Zink (DE-588)4137453-8 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000096223-4 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000096223 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027163102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Martin, Lena Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets probiotics cabt Enterococcus faecium cabt pigs cabt weaning cabt zinc oxide cabt digestive system cabt piglets cabt jejunum cabt enzymes cabt reverse transcriptase PCR cabt gene expression cabt Futterzusatz (DE-588)4155774-8 gnd Zink (DE-588)4137453-8 gnd Dünndarm (DE-588)4013218-3 gnd Probiotikum (DE-588)4338112-1 gnd Ferkel (DE-588)4154007-4 gnd |
| subject_GND | (DE-588)4155774-8 (DE-588)4137453-8 (DE-588)4013218-3 (DE-588)4338112-1 (DE-588)4154007-4 (DE-588)4113937-9 |
| title | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets |
| title_alt | Studien zum Einfluss des Probiotikums Enterococcus faecium NCIMB 10415 und des Spurenelements Zink auf Leistung und diverse verdauungsphysiologische Parameter beim Ferkel |
| title_auth | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets |
| title_exact_search | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets |
| title_full | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets vorgelegt von Lena Martin |
| title_fullStr | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets vorgelegt von Lena Martin |
| title_full_unstemmed | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets vorgelegt von Lena Martin |
| title_short | Studies on the influence of the probiotic Enterococcus faecium NCIMB 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets |
| title_sort | studies on the influence of the probiotic enterococcus faecium ncimb 10415 and the trace element zinc on performance and digestive physiological parameters in the small intestine of piglets |
| topic | probiotics cabt Enterococcus faecium cabt pigs cabt weaning cabt zinc oxide cabt digestive system cabt piglets cabt jejunum cabt enzymes cabt reverse transcriptase PCR cabt gene expression cabt Futterzusatz (DE-588)4155774-8 gnd Zink (DE-588)4137453-8 gnd Dünndarm (DE-588)4013218-3 gnd Probiotikum (DE-588)4338112-1 gnd Ferkel (DE-588)4154007-4 gnd |
| topic_facet | probiotics Enterococcus faecium pigs weaning zinc oxide digestive system piglets jejunum enzymes reverse transcriptase PCR gene expression Futterzusatz Zink Dünndarm Probiotikum Ferkel Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000096223 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027163102&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT martinlena studiesontheinfluenceoftheprobioticenterococcusfaeciumncimb10415andthetraceelementzinconperformanceanddigestivephysiologicalparametersinthesmallintestineofpiglets AT martinlena studienzumeinflussdesprobiotikumsenterococcusfaeciumncimb10415unddesspurenelementszinkaufleistungunddiverseverdauungsphysiologischeparameterbeimferkel |