Homing endonucleases: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1123 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | X, 284 S. Ill., graph. Darst. |
| ISBN: | 1627039678 9781627039673 |
Internformat
MARC
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| 264 | 1 | |a New York [u.a.] |b Humana Press |c 2014 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-027160087 | ||
Datensatz im Suchindex
| _version_ | 1804151986387419136 |
|---|---|
| adam_text | Contents
Preface
.......................................................... v
Contributors
.......................................................... ix
1
Homing Endonucleases: From Genetic Anomalies
to Programmable Genomic Clippers
................................ 1
Marlene
Belfort
and Richard P. Bonocora
2
Bioinformatic Identification of Homing Endonucleases
and Their Target Sites
........................................... 27
Eyal Privman
3
PCR-Based Bioprospecting for Homing Endonucleases
in Fungal Mitochondrial rRNA Genes
............................... 37
Mohamed
Hafez, Tuhin Kumar Guha, Chen Shen,
Jyothi Sethuraman, and
Georg Hausner
4
Mapping Homing Endonuclease Cleavage Sites Using
In Vitro Generated Protein
....................................... 55
Richard P.
Bonocora and
Marlene
Belfort
5
Mapping Free-Standing Homing Endonuclease Promoters
Using 5 RLM-RACE
........................................... 69
Ewan A. Gibb
6
PCR Analysis of
Chloroplast
Double-Strand Break (DSB) Repair
Products Induced by I-Crell in Chlamydomonas and Arabidopsis
.......... 77
Taegun Kwon,
Obed
W. Odom,
Weihua Qiu, and David L·
Herrin
7
A Two-Plasmid Bacterial Selection System for Characterization
and Engineering of Homing Endonucleases
.......................... 87
Шца
Sun and Huimin Zhao
8
Rapid Screening of Endonuclease Target Site Preference
Using a Modified Bacterial Two-Plasmid Selection
..................... 97
Jason M.
Wolfi,
Benjamin P. Kleinstiver, and David R. Edgell
9
A Yeast-Based Recombination Assay for Homing Endonuclease Activity
..... 105
Jean-Charles Epinat
10
Rapid Determination of Homing Endonuclease
DNA
Binding Specificity Profile
........................................ 127
Lei Zhao and Barry L. Stoddard
11
Quantifying the Information Content of Homing Endonuclease
Target Sites by Single Base Pair Profiling
............................. 135
Joshua I. Friedman, Hut Li, and Raymond J. Monnatjr.
viii Contents
12
Homing Endonuclease Target Site Specificity Defined
by Sequential Enrichment and Next-Generation Sequencing
of Highly Complex Target Site Libraries
............................. 151
Hui
Li and Raymond J. Monnatjr.
13
Homing Endonuclease Target Determination Using SELEX
Adapted for Yeast Surface Display
.................................. 165
KyUJacoby and Andrew M.
Scharenberg
14
Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG
Homing Endonucleases from Homologous I-Onul-Family Enzymes
........ 191
Sarah K. Baxter, Andrew M.
Scharenberg,
and Abigail R. Lambert
15
Bioinformatics Identification of Coevolving Residues
................... 223
Russell J.
Dickson
and Gregory B.
Gloor
16
Identification and Analysis of Genomic Homing
Endonuclease Target Sites
........................................ 245
Stefan Pellenz and Raymond J. Monnatjr.
17
Redesigning the Specificity of Protein-DNA Interactions with
Rosetta
...... 265
Summer Thyme and David Baker
Index
............................................................... 283
Methods in Molecular
Biology
1123
John
M,
Walker,
Seríes
Editor
David
R. Edgelí Editor
Homing Endonucleases
Methods and Protocols
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with
a comprehensive resource to identify and characterize homing endonucleases from genomic
sequence» to deduce the biological basis of binding and cleavage specificity, as well as to provide
protocols to redesign endonuclease target specificity for genome-editing applications. Engineer¬
ing of designer homing endonucleases has set the stage for genome editing of complex eukary-
otic genomes with a broad range of potential applications including targeted gene knockouts in
model organisms and gene therapy in humans, making this book a valuable resource for future
research. Written in the highly successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoid¬
ing known pitfalls.
Authoritative and practical, Homing Endonucieases: Methods ana
Proto
coh serves as
â
key refer¬
ence for all kbs studying site-specific
DNA
endonucleases.
|
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| dewey-full | 572.8 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8 |
| dewey-search | 572.8 |
| dewey-sort | 3572.8 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| indexdate | 2024-07-10T01:03:32Z |
| institution | BVB |
| isbn | 1627039678 9781627039673 |
| language | English |
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| physical | X, 284 S. Ill., graph. Darst. |
| publishDate | 2014 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Homing endonucleases methods and protocols ed. by David R. Edgell New York [u.a.] Humana Press 2014 X, 284 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1123 Springer protocols Endonucleasen (DE-588)4152179-1 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Endonucleasen (DE-588)4152179-1 s Labortechnik (DE-588)4123602-6 s DE-604 Edgell, David R. Sonstige oth Erscheint auch als Online-Ausgabe 978-1-62703-968-0 Methods in molecular biology 1123 (DE-604)BV035362695 1123 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027160087&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027160087&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Homing endonucleases methods and protocols Methods in molecular biology Endonucleasen (DE-588)4152179-1 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4152179-1 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Homing endonucleases methods and protocols |
| title_auth | Homing endonucleases methods and protocols |
| title_exact_search | Homing endonucleases methods and protocols |
| title_full | Homing endonucleases methods and protocols ed. by David R. Edgell |
| title_fullStr | Homing endonucleases methods and protocols ed. by David R. Edgell |
| title_full_unstemmed | Homing endonucleases methods and protocols ed. by David R. Edgell |
| title_short | Homing endonucleases |
| title_sort | homing endonucleases methods and protocols |
| title_sub | methods and protocols |
| topic | Endonucleasen (DE-588)4152179-1 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Endonucleasen Labortechnik Aufsatzsammlung |
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