Endosome signaling: B
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2014
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
535 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXI, 480 S., [7] Bl. - Ill., graph. Darst. |
| ISBN: | 9780123979254 |
Internformat
MARC
| LEADER | 00000nam a2200000 cc4500 | ||
|---|---|---|---|
| 001 | BV041656045 | ||
| 003 | DE-604 | ||
| 005 | 20150226 | ||
| 007 | t | ||
| 008 | 140218s2014 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123979254 |9 978-0-12-397925-4 | ||
| 035 | |a (OCoLC)873507872 | ||
| 035 | |a (DE-599)BVBBV041656045 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-19 | ||
| 245 | 1 | 0 | |a Endosome signaling |n B |c ed. by P. Michael Conn |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2014 | |
| 300 | |b XXI, 480 S., [7] Bl. - Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 535 | |
| 490 | 0 | |a Methods in enzymology |v ... | |
| 700 | 1 | |a Conn, Patrick Michael |d 1949-2016 |e Sonstige |0 (DE-588)136758541 |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV041655920 |g 2 |
| 830 | 0 | |a Methods in enzymology |v 535 |w (DE-604)BV000000938 |9 535 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg - ADAM Catalogue Enrichment |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027096541&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-027096541 | ||
Datensatz im Suchindex
| _version_ | 1804151887136555008 |
|---|---|
| adam_text | CONTENTS
Contributors
xiii
Preface
xxi
Section I
SIGNALING
1.
Assessment of Insulin Proteolysis in Rat Liver Endosomes:
Its Relationship to Intracellular Insulin Signaling
3
François Authier
and Bernard Desbuquois
1.
Introduction
4
2.
Assays for Insulin Degradation
5
3.
Assay for Endosomal Proteolysis of Insulin
7
4.
Sites of Cleavage and Degradation Products of Insulin within Endosomes
12
5.
Endosomai
Insulinases
14
6.
Effects of Insulin and Slowly Processed Insulin Analogs on Insulin Signaling
in Liver Endosomes
15
7.
Involvement of
IR
Endocytosis in Positive Regulation of Insulin
Signaling
20
References
21
2.
A Bimolecular Fluorescent Complementation Screen Reveals
Complex Roles of Endosomes in Ras-Mediated Signaling
25
Ze-Yi Zheng and Eric
С
Chang
1.
Introduction
26
2.
Detect Ras-effector Interactions by BiFC
30
3.
Screen for Ras-binding Proteins by BiFC
33
4.
Concluding Remarks
36
Acknowledgments
37
References
37
3. TGFß in
Endosomal Signaling
39
Sarah McLean and Gianni M.
Di Guglielmo
1.
Introduction
40
2.
Endocytosis
40
3.
The Role of the Early
Endosóme
in
TGFß
Signal Transduction
45
vi
Contents
4.
Readouts of
TGFß
Signal Transduction
50
Acknowledgments
52
References
52
4.
Annexins and Endosomal Signaling
55
Francese
Tebar, Mariona Gelabert-Baldrich,
Mónira
Hoque,
Rose Cairns,
Carles
Rentero,
Albert Pol, Thomas
Grewał,
and Carlos Enrich
1.
Introduction
56
2.
Isolation of Endocytic Compartments
59
3.
Targeting Raf-1 Signaling to Early Endosomes
63
4.
Monitoring Endosomal Signaling by Fluorescence Resonance
Energy Transfer Microscopy
68
5.
Targeting Annexins to Endosomes and Other Cellular Compartments
69
6.
Summary
70
Acknowledgments
70
References
71
5.
Analysis, Regulation, and Roles of Endosomal Phosphoinositides
75
Tania Maffucci
and Marco Falasca
1.
Introduction
76
2.
Endosomal Pis
76
3.
Analysis of Ptdlns3P Levels
78
4.
Monitoring Ptdlns3P Intracellular Localization
82
5.
Endosomal Ptdlns3P
85
6.
Phosphatidylinositol 3,5-Bisphosphate
87
Acknowledgments
88
References
88
6.
Mild Fixation and Permeabilization Protocol for Preserving
Structures of Endosomes, Focal Adhesions, and Actin
Filaments During Immunofluorescence Analysis
93
Julia M. Scheffler, Natalia
Schiefermeier,
and
Lukas
A. Huber
1.
introduction
94
2.
Molecular Tools
95
3.
Saponin Treatment Enhances the Preservation of Peripheral Endosomes
After Fixation
96
4.
Mild Fixation Allows Preserving the mCherry and GFP Localized
to Focal Adhesions and Late Endosomes
98
5.
Summary
101
Contents
vii
Acknowledgment
101
References
101
7.
Characterizing and Measuring Endocytosis of Lipid-Binding
Effectors in Mammalian Cells
103
Helen R. Clark, Tristan A. Hayes, and Shiv D. Kale
1.
Introduction
104
2.
Culturing and Maintenance of Mammalian Cell Lines
105
3.
Transfection of Mammalian Cells
106
4.
Protein Purification and Preparation
108
5.
Treatment of Celts
110
6.
Tracking Endocytosis by Confocai Microscopy
110
7.
Quantification of Ceil Entry by Fluorescent Microtiter Plate Reader
113
8.
Quantification of Cell Entry by Flow Cytometry
115
9.
Summary
Π
7
Acknowledgments
118
References
118
8.
Measuring the Role for Met Endosomal Signaling
in Tumorigenesis
121
Rachel Barrow,
Carine Joffre,
Ludovic Ménard,
and
Stéphanie Kermorgant
1.
Introduction
122
2.
Analyzing Met Trafficking with Fluorescence and Confocai Microscopy
123
3.
Analyzing Met
Internalizaron
Using Flow Cytometry
127
4.
Analyzing Met
Internalizaron,
Recycling, and Degradation with
Surface Met Biotinylation
130
5.
Analyzing Met Endosomal Signaling In Vitro and In Vivo
134
6.
Summary
138
References
139
9.
Intracellular Toll-Like Receptor Recruitment and
Cleavage in Endosomal/Lysosomal
Organelies
141
Mira Tohmé
and
Bénédicte Manoury
1.
Introduction
142
2.
Purification of Endosomes and Lysosomes
142
3.
Proteases Assays
145
4.
Intracellular TLR Processing
145
References
146
viii Contents
10.
Assessment of the Toll-Like Receptor
3
Pathway in
Endosomal Signaling
149
Misako Matsumoto, Kenji
Funami, Megumi
Tatematsu, Masahiro
Azuma,
and Tsukasa Seya
1.
Introduction
150
2.
Analyses of TLR3 Expression and Localization
151
3.
Assay for TLFG-Mediated Signaling
154
4.
Assay for DC-Mediated NK Activation
158
5.
Assay for DC-Mediated CTL Activation
160
6.
Summary
162
Acknowledgments
162
References
162
11.
Labeling of Platelet-Derived Growth Factor by Reversible
Biotinylation to Visualize Its Endocytosis by Microscopy
167
Łukasz
Sadowski,
Kamil Jastrzębski, Elżbieta
Purta,
Carina
Hellberg,
and Marta
Miaczynska
1.
Introduction
168
2.
PDGF Labeling with Sulfo-A/ Hydroxysuccinimide-SS-Biotin
169
3.
Determination of the Extent of PDGF Biotinylation with Mass Spectrometry
171
4.
Determination of a Biological Activity of bt-PDGF
172
5.
Stimulation of Cells with bt-PDGF and Removal of Extracellular Biotins
173
6.
Validation and Detection of bt-PDGF in Microscopical Assays
174
7.
Conclusion
175
Acknowledgments 1
76
References
176
12.
Endosomal Signaling and Oncogenesis
179
Nikolai Engedal and
lan G.
Mills
1.
Introduction
180
2.
Receptors
181
3.
Adaptor Proteins and Oncogenesis
188
References
195
13.
ROS-Containing Endosomal Compartments: Implications
for Signaling
201
A. Paige Davis
Volk
and Jessica
G. Moreland
1.
Introduction
202
2.
Overview of Endocytosis
203
Contents
¡χ
3.
Sources
of
Endosomal
ROS
204
4.
Measurement of
Endosomal
ROS
205
5.
Fluorescent Probes
209
6.
Chemiluminescent Probes
216
7.
Emerging
ROS
Probes
220
8.
Summary
221
Acknowledgment
221
References
222
14.
SARA and RNF11 at the Crossroads of EGFR Signaling
and Trafficking
225
Eleftherios
Kostaras,
Nina Marie Pedersen,
Harald Stenmark,
Theodore Fotsis, and Carol Murphy
1.
Introduction
226
2.
RNF1
1
¡ntraceilular Compartmentalization
226
3.
Biochemical Analysis of Interacting Proteins
231
4.
Tracking EGF Ligand-Receptor Complex Dynamics
238
5.
Summary
244
Acknowledgments
245
References
246
15.
P18/LAMTOR1: A Late Endosome/Lysosome-Specific
Anchor Protein for the mTORCI/MAPK Signaling Pathway
249
Shigeyuki
Nada,
Shunsuke Mori, Yusuke Takahashi, and Masato Okada
1.
Introduction
250
2.
Identification of pi
8 250
3.
Intracellular Localization of p1
8 253
4.
Identification of pie-Interacting Proteins
254
5.
Functional Analysis of p1
8 256
6.
Summary and Perspective
262
Acknowledgments
262
References
263
16.
Vascular Endothelial Growth Factor
А
-Stimulated Signaling from
Endosomes in Primary Endothelial Cells
265
Gareth W. Fearnley,
Gina
A. Smith, Adam F. Odeil, Antony M. Latham,
Stephen B. Wheatcroft, Michael A. Harrison, Darren C. Tomlinson,
and Sreenivasan Ponnambaiam
1.
Overview
266
X
Contents
2. Endothelial
Cell Characterization
268
3.
Reverse Genetics
273
4.
VEGFR Trafficking, Signaling, and Proteolysis
276
5.
Endothelial Cell Responses
280
6.
Tubulogenesis
285
7.
Summary
288
Acknowledgments
290
References
290
17.
Assessment of
Internalizaron
and Endosomal Signaling:
Studies with Insulin and EGF
293
Barry I. Posner and John J. M. Bergeron
1.
Introduction
294
2.
Endosomal Signaling by Insulin
294
3.
Endosomal Signaling by Epidermal Growth Factor
302
4.
Summary
305
References
305
18.
Quantitative Proteomic Analysis of Compartmentalized
Signaling Networks
309
Maria
Hernández-Valladares,
Veronica
Aran,
and Ian A. Prior
1.
Introduction
310
2.
Compartmentalized
Ras
Constructs
310
3.
Stable Cell Line Generation
311
4.
Proteomic Analysis of Compartment-Specific Signaling
313
5.
Summary
322
Acknowledgments
322
References
323
19.
Separation of Magnetically Isolated TNF Receptosomes
from Mitochondria
327
Vladimir Tchikov,
Jürgen Fritsch,
and Stefan
Schütze
1.
Introduction
328
2.
Physical Background
332
3.
Reagents and Cells
334
4.
Protocol
1 :
Magnetic Separation
335
5.
Protocol
2:
Separation by lodixanol Density Barrier Centrifugation
340
6.
Results
341
7.
Conclusions
345
Contents
xi
Acknowledgments 346
References
346
20.
Deubiquìtinases
and Their Emerging Roles in
ß-Arrestin-Mediated
Signaling
351
Sudha K. Shenoy
1.
Introduction
352
2.
Interaction of
ß-Arrestin2
with the Deubiquitinase USP33
354
3.
Assay of USP33 Activity in Vitro
357
4.
Effects of LJSP33 Overexpression
361
5.
Effects of USP33 Knockdown
365
6.
Summary
369
Acknowledgments
369
References
369
21.TSLP Expression Induced via Toll-Like Receptor
Pathways in Human Keratinocytes
371
Toshiro Takai, Xue Chen, Yang Xie, Anh Tuan
Vu, Tuan Anh Le,
Hirokazu Kinoshita, Junko Kawasaki, Seiji
Kamijo, Mutsuko Hara,
Hiroko Ushio, Tadashi
Baba,
Keiichi Hiramatsu, Shigaku Ikeda,
Hideoki Ogawa, and
Ko Okumura
1.
Introduction
372
2.
Reagents for Stimulation or Treatment of Human KCs
373
3.
Cell Culture and Stimulation of Human KCs
375
4.
Analytical Methods
377
5.
TLR-Mediated Pathways of TSLP Induction in Human KCs
380
6.
Long TSLP Transcript Responsible to Expression of the TSLP Protein
383
7.
Summary
384
Acknowledgments
385
References
385
22.
Endosomal Signaling by Protease-Activated Receptors
389
Neil Grimsey, Huilan Lin, and JoAnn Trejo
1.
Introduction
390
2.
Imaging of p38,
ERKI
/2,
and PAR1 on Endosomes
391
3. ß-Arrestin
Recruitment to Endosomes
394
4.
Rab5 Q79L Expansion of Early Endosomes
397
5.
Immunopredpitation of
PARI
Signaling Complexes
397
6.
Summary
399
xii Contents
Acknowledgments
400
References
400
23.
Investigating Signaling Consequences of GPCR Trafficking
in the Endocytic Pathway
403
Roshanak Irannejad, Sarah J. Kotowski, and Mark
von Zastrow
1.
Introduction
404
2.
Luminescence-Based Assay of Acute cAMP Regulation in Cell Populations
405
3.
FRET Imaging of Acute cAMP Regulation in Individual Cells
408
4.
Experimental Manipulation of GPCR Endocytic Trafficking
412
5.
Summary
416
Acknowledgments
417
References
417
24.
MICAL-Likei in Endosomal Signaling
419
Ahmed Zahraoui
1.
Introduction
420
2.
Methods
424
3.
Conclusion
435
Acknowledgments
435
References
435
Author Index
439
Subject Index
467
|
| any_adam_object | 1 |
| author_GND | (DE-588)136758541 |
| building | Verbundindex |
| bvnumber | BV041656045 |
| ctrlnum | (OCoLC)873507872 (DE-599)BVBBV041656045 |
| edition | 1. ed. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01282nam a2200325 cc4500</leader><controlfield tag="001">BV041656045</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20150226 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">140218s2014 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123979254</subfield><subfield code="9">978-0-12-397925-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)873507872</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV041656045</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-19</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Endosome signaling</subfield><subfield code="n">B</subfield><subfield code="c">ed. by P. Michael Conn</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Acad. Press</subfield><subfield code="c">2014</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="b">XXI, 480 S., [7] Bl. - Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">535</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">...</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Conn, Patrick Michael</subfield><subfield code="d">1949-2016</subfield><subfield code="e">Sonstige</subfield><subfield code="0">(DE-588)136758541</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="w">(DE-604)BV041655920</subfield><subfield code="g">2</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in enzymology</subfield><subfield code="v">535</subfield><subfield code="w">(DE-604)BV000000938</subfield><subfield code="9">535</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg - ADAM Catalogue Enrichment</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027096541&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-027096541</subfield></datafield></record></collection> |
| id | DE-604.BV041656045 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:01:57Z |
| institution | BVB |
| isbn | 9780123979254 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027096541 |
| oclc_num | 873507872 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-19 DE-BY-UBM |
| owner_facet | DE-355 DE-BY-UBR DE-19 DE-BY-UBM |
| physical | XXI, 480 S., [7] Bl. - Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Endosome signaling B ed. by P. Michael Conn 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2014 XXI, 480 S., [7] Bl. - Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 535 Methods in enzymology ... Conn, Patrick Michael 1949-2016 Sonstige (DE-588)136758541 oth (DE-604)BV041655920 2 Methods in enzymology 535 (DE-604)BV000000938 535 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027096541&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Endosome signaling Methods in enzymology |
| title | Endosome signaling |
| title_auth | Endosome signaling |
| title_exact_search | Endosome signaling |
| title_full | Endosome signaling B ed. by P. Michael Conn |
| title_fullStr | Endosome signaling B ed. by P. Michael Conn |
| title_full_unstemmed | Endosome signaling B ed. by P. Michael Conn |
| title_short | Endosome signaling |
| title_sort | endosome signaling |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027096541&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV041655920 (DE-604)BV000000938 |
| work_keys_str_mv | AT connpatrickmichael endosomesignalingb |