Protein downstream processing: design, development and application of high and low-resolution methods
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1129 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 555 S. Ill., graph. Darst. |
| ISBN: | 9781627039765 |
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| 245 | 1 | 0 | |a Protein downstream processing |b design, development and application of high and low-resolution methods |c Ed. by Nikolaos E.Labrou |
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Datensatz im Suchindex
| _version_ | 1804151862628188160 |
|---|---|
| adam_text | Titel: Protein downstream processing
Autor: Labrou, Nikolaos E
Jahr: 2014
Contents
Preface............................................................................................................................. v
Contributors.................................................................................................................... xi
Part I Screening and Design Purification Strategies
1 Protein Purification: An Overview.................................................................... 3
Nikolaos E. Labrou
2 Establishment of a Design Space for Biopharmaceutical Purification
Processes Using DoE....................................................................................... 11
Ignacio Amadeo, Laura Mauro, Eduardo Orti, and Guillermina Forno
3 High-Throughput Process Development: I. Process Chromatography.............. 29
Anurag S. Rathore and Rahul Bhambure
4 High-Throughput Process Development: II. Membrane Chromatography....... 39
Anurag S. Rathore and Sampath Muthukumar
5 Media Selection in Ion-Exchange Chromatography in a Single Microplate........ 45
Charlotte Cabanne and Xavier Santarelli
6 High-Throughput Screening of Dye-Ligands for Chromatography................... 53
Sunil Kumar and Narayan S. Punekar
7 Measurement of Uptake Curves and Adsorption Isotherms
by Automated Microscale Chromatography Pipette Tips.................................. 67
Sunil Chhatre and Daniel G. Bracewell
Part II Low-Resolution Protein Purification Methods
8 Recovery of Recombinant Proteins from Plants Using Aqueous
Two-Phase Partitioning Systems: An Outline................................................... 77
Zhengrong Gu
9 Aqueous Two-Phase System Strategies for the Recovery
of Proteins from Plants..................................................................................... 89
Oscar Aguilar and Marco Rito-Palomares
10 Aqueous Two-Phase-Assisted Precipitation of Proteins:
A Platform for Isolation of Process-Related Impurities
from Therapeutic Proteins................................................................................ 101
Anurag S. Rathore and Rahul Bhambure
11 Simultaneous Purification and Refolding of Proteins by Affinity
Precipitation and Macro (Affinity Ligand)-Facilitated Three Phase
Partitioning (MLFTPP)................................................................................... Ill
Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta
vii
viii
Contents
12 Co-expression and Co-purification of Antigen-Antibody
Complexes in Bacterial Cytoplasm and Periplasm.............................................. 125
Ario de Marco
13 Immunoglobulin Purification by Caprylic Acid................................................. 137
Victor Morais, Patricia Berasain, and Hugo Massaldi
Part III High-Resolution Protein Purification Methods
14 Affinity Tags in Protein Purification
and Peptide Enrichment: An Overview............................................................. 147
Ana Sofia Pina, Iris L. Batalha, and Ana Cecilia A. Roque
15 Expression and Purification of Large Active GST Fusion Enzymes.................... 169
Stefania Deceglie, Claudia Lionetti, Marina Roberti,
Palmiro Cantatore, and Paola Loguercio Polosa
16 Synthetic Ligand Affinity Chromatography Purification
of Human Serum Albumin and Related Fusion Proteins................................... 181
Sharon Williams, Phil Morton, and Dev Baines
17 ZbasiC: A Purification Tag for Selective Ion-Exchange Recovery.......................... 197
My Hedhammar, Johan Nilvebrant, and Sophia Hober
18 An Orthogonal Fusion Tag for Efficient Protein Purification............................ 205
Johan Nilvebrant, Mikael AsPrand, and Sophia Hober
19 Phage Display of Engineered Binding Proteins................................................. 211
Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel,
Serve W.M. Kengen, and John van der Oost
20 Biomimetic Affinity Ligands for Protein Purification........................................ 231
Isabel T. Sousa and M. Angela Taipa
21 Synthesis and Application of Dye-Ligand Affinity Adsorbents........................... 263
Evangelia Chronopoulou and Nikolaos E. Labrou
22 Peptide Affinity Chromatography Based on Combinatorial
Strategies for Protein Purification..................................................................... 277
Silvia Andrea Camperi, Maria Camila Martinez-Ceron,
Silvana Laura Giudicessi, Mariela Mirta Marani, Fernando Albericio,
and Osvaldo Cascone
23 Affinity Chromatography of Proteins on Monolithic Columns.......................... 303
E.G. Vlakh, G.A. Platonova, and T.B. Tennikova
24 Sample Displacement Batch Chromatography of Proteins................................. 325
Marta Kotasinska, Verena Richter, Marcel Kwiatkowski,
and Hartmut Schliiter
Part IV Assessing Protein Structural Integrity,
Purity, and Stabilization
25 Analysis of Host-Cell Proteins in Bio therapeutic Proteins
by LC/MS Approaches...................................................
Catalin E. Doneanu and Weibin Chen
341
Contents ix
26 Preparation of Monolithic Affinity Media for Nano-Liquid
Chromatography Applications.......................................................................... 351
Jens Sprofi and Andrea Sinz
27 Proteomic Analysis of Complex Protein Samples
by MALDI-TOF Mass Spectrometry................................................................ 365
Cosima Damiana Calvano, Cristina De Ceglie, and Carlo G. Zambonin
28 Modern Bioanalysis of Proteins by Electrophoretic Techniques......................... 381
Sona Krizkova, Marketa Ryvolova, Miehal Masarik, Ondrej Zitka,
Vojtech Adam, Jaromir Hubalek, Tomas Eckschlager, and Rene Kizek
29 Protein Structure Validation and Analysis with X-Ray Crystallography.............. 397
Anastassios C. Papageorgiou and Jesse Mattsson
30 Measuring Binding Constants of His-Tagged Proteins
Using Affinity Chromatography and Ni-NTATmmobilized Enzymes................ 423
Annette C. Moser, Benjamin White, and Frank A. Kovacs
31 Stabilization of Therapeutic Proteins in Aqueous Solutions
and Freeze-Dried Solids: An Overview............................................................. 435
Ken-ichi Izutsu
32 Stabilization of Protein by Freeze-Drying in the Presence
of Trehalose: A Case Study of Tubulin.............................................................. 443
Pavel Drdber, Vadym Sulimenko, Tetyana Sulimenko,
and Eduarda Drdberovd
Part V Applications/Case Studies
33 G-Protein-Coupled Receptor Expression and Purification................................. 461
Karolina Corin, Lotta T. Teller, and Sotirios Koutsopoulos
34 (Hyper)thermophilic Enzymes: Production and Purification............................. 487
Pierpaolo Falcicchio, Mark Levisson, Serve W.M. Kengen,
Sotirios Koutsopoulos, and John van der Oost
35 Affinity Chromatography for Antibody Purification.......................................... 497
Sushrut Arora, B. Vijayalakshmi Ayyar, and Richard O Kennedy
36 Screening and Purification of Recombinant Lignocellulolytic Enzymes............. 517
Evangelos Topakas and Paul Christakopoulos
37 Purification of PEGylated Proteins, with the Example
of PEGylated Lysozyme and PEGylated scFv.................................................... 527
Anna Moosmann, Egbert Miiller, and Heiner Bottinger
38 One-Step Purification of Glutamate Decarboxylase from E. coli
Using Aqueous Two-Phase System................................................................... 539
Wanying Tao, Jun Zhu, and Bo Sun
Index............................................................................................................................... 547
|
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| author2 | Labrou, Nikolaos E. |
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| discipline | Chemie / Pharmazie Biologie |
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| spelling | Protein downstream processing design, development and application of high and low-resolution methods Ed. by Nikolaos E.Labrou New York [u.a.] Humana Press 2014 XV, 555 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1129 Springer protocols Reinigung (DE-588)4049250-3 gnd rswk-swf Trennverfahren (DE-588)4078395-9 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Reinigung (DE-588)4049250-3 s DE-604 Trennverfahren (DE-588)4078395-9 s b DE-604 Labrou, Nikolaos E. edt Methods in molecular biology 1129 (DE-604)BV035362695 1129 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027081093&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein downstream processing design, development and application of high and low-resolution methods Methods in molecular biology Reinigung (DE-588)4049250-3 gnd Trennverfahren (DE-588)4078395-9 gnd Proteine (DE-588)4076388-2 gnd |
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| title | Protein downstream processing design, development and application of high and low-resolution methods |
| title_auth | Protein downstream processing design, development and application of high and low-resolution methods |
| title_exact_search | Protein downstream processing design, development and application of high and low-resolution methods |
| title_full | Protein downstream processing design, development and application of high and low-resolution methods Ed. by Nikolaos E.Labrou |
| title_fullStr | Protein downstream processing design, development and application of high and low-resolution methods Ed. by Nikolaos E.Labrou |
| title_full_unstemmed | Protein downstream processing design, development and application of high and low-resolution methods Ed. by Nikolaos E.Labrou |
| title_short | Protein downstream processing |
| title_sort | protein downstream processing design development and application of high and low resolution methods |
| title_sub | design, development and application of high and low-resolution methods |
| topic | Reinigung (DE-588)4049250-3 gnd Trennverfahren (DE-588)4078395-9 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Reinigung Trennverfahren Proteine Aufsatzsammlung |
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