Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | 113 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 |c [presented by: Qing Chen] |
| 264 | 1 | |c 2014 | |
| 300 | |a 113 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 500 | |a Zsfassung in dt. Sprache | ||
| 502 | |a Heidelberg, Univ., Diss., 2014 | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-027080386 | ||
Datensatz im Suchindex
| _version_ | 1804151861439102976 |
|---|---|
| adam_text | TABLE OF CONTENTS
ABBREVIATIONS 5
LIST OF FIGURES 7
LIST OF TABLES 9
1. SUMMARY 10
ZUSAMMENFASSUNG 11
2. INTRODUCTION 13
2.1
OVERVIEW OF WNT
SIGNALING 13
2.2 WNTS
AND FRIZZLED RECEPTORS 16
2.3 CANONICAL
AND NON-CANONICAL WNT SIGNALING 16
2.4 REGULATION OF WNT/P-CATENIN SIGNALING 18
2.5 THE
WNT CO-RECEPTOR LRP5/6: PHYSIOLOGICAL RELEVANCE 19
2.6
STRUCTURAL FEATURES OF LRP5/6 20
2.7 LRP6 REGULATION ON WNT SIGNALING 23
2.8 LRP6
AND WNT SIGNALING DOWNSTREAM OF RECEPTOR ACTIVATION 30
2.9
SRC
FAMILY KINASE IN DEVELOPMENT AND DISEASE 32
2.10 SRC
AND WNT/P-CATENIN SIGNALING 35
3. AIM
OF THE
STUDY 38
4. RESULTS 40
4.1.
LRP6 HARBORS EVOLUTIONARILY CONSERVED TYROSINE RESIDUES 40
4.2 A CDNA BASED EXPRESSION
SCREEN IDENTIFIED SRC
AS AN LRP6
TYROSINE KINASE
40
4.3
SRC
FAMILY KINASES PHOSPHORYLATE LRP6 IN A KINASE DEPENDENT MANNER 42
4.4
TYROSINE PHOSPHORYLATION INHIBITS LRP6 ACTIVITY AND WNT SIGNALING 44
4.5
SRC
INHIBITS LRP6 SIGNALING IN VIVO 48
4.6
SRC
INTERACTS WITH LRP6 50
4.7
SRC PHOSPHORYLATES MULTIPLE LRP6TYROSINE RESIDUES 51
4.8
WNT
SIGNALING INDUCES LRP6
TYROSINE PHOSPHORYLATION 57
4.9 LRP6
PPPSP MOTIFS
PRIMING TYROSINE PHOSPHORYLATION 59
4.10
SRC REDUCES LRP6 PLASMA MEMBRANE ABUNDANCE 63
4.11
SRC PROMOTES LRP6 TURN OVER 66
5. DISCUSSION 69
3
HTTP://D-NB.INFO/1047469294
5.1
LRP6 TYROSINE PHOSPHORYLATION 70
5.2 TYROSINE PHOSPHORYLATION INHIBITS LRP6 ACTIVITY 73
5.3 REGULATION OF SRC
MEDIATED LRP6 PHOSPHORYLATION 75
5.4
SRC REDUCES LRP6 MEMBRANE ABUNDANCE 76
5.5
SRC
AND LRP6 HALF-LIFE 77
5.6 MULTIPLE
CROSS TALKS BETWEEN SRC
AND WNT SIGNALING 77
5.7 IN VIVO
SIGNIFICANCE OF SRC MEDIATED LRP6 PHOSPHORYLATION 78
6. MATERIALS
AND METHODS 80
6.1
CELL LINES
AND CULTURE CONDITIONS 80
6.2 TRANSFECTIONS 81
6.3 LUCIFERASE ASSAY AND WESTERN BLOT 84
6.4
GENOME-WIDE CDNA EXPRESSION
SCREENING FOR LRP6 MODIFIERS 86
6.5 IMMUNOPRECIPITATION 86
6.6 IN VITRO
KINASE ASSAY 87
6.6 MEMBRANE FRACTIONATION 87
6.7 CELL
SURFACE PROTEIN BIOTINYLATION 87
6.8
CYCLOHEXIMIDE(CHX)-CHASE ASSAY
FOR LRP6 HALF-LIFE
STUDY 88
6.9 INDIRECT IMMUNOFLUORESCENCE STAINING AND MICROSCOPY 89
6.10 GENERAL MOLECULAR BIOLOGY METHODS 90
6.11
SITE-DIRECTED MUTAGENESIS 90
6.12 REAL-TIME PCR 93
6.13 ZEBRAFISH MANIPULATING, MRNA INJECTION AND IN SITU HYBRIDIZATION 93
6.14 EQUIPMENT 96
6.15 ANTIBODIES 97
6.16 OTHER MATERIALS AND REAGENTS 98
ACKNOWLEDGEMENTS 99
PUBLICATIONS: 101
REFERENCE: 102
4
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| any_adam_object | 1 |
| author | Chen, Qing |
| author_GND | (DE-588)1047085828 |
| author_facet | Chen, Qing |
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| dewey-full | 571.74 |
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| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.74 |
| dewey-search | 571.74 |
| dewey-sort | 3571.74 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T01:01:33Z |
| institution | BVB |
| language | English German |
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| spelling | Chen, Qing Verfasser (DE-588)1047085828 aut Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 [presented by: Qing Chen] 2014 113 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache Heidelberg, Univ., Diss., 2014 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027080386&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chen, Qing Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 |
| subject_GND | (DE-588)4113937-9 |
| title | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 |
| title_auth | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 |
| title_exact_search | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 |
| title_full | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 [presented by: Qing Chen] |
| title_fullStr | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 [presented by: Qing Chen] |
| title_full_unstemmed | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 [presented by: Qing Chen] |
| title_short | Tyrosine phosphorylation and regulation of Wnt co-receptor LRP6 |
| title_sort | tyrosine phosphorylation and regulation of wnt co receptor lrp6 |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027080386&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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