Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Jülich
Forschungszentrum Jülich, Zentralbibliothek
2013
|
| Series: | Schriften des Forschungszentrums Jülich
Reihe Gesundheit ; 66 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Zsfassung in dt. Sprache |
| Physical Description: | 127 S. Ill., graph. Darst. |
| ISBN: | 9783893369003 |
Staff View
MARC
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| 245 | 1 | 0 | |a Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |c Solvej Siedler |
| 264 | 1 | |a Jülich |b Forschungszentrum Jülich, Zentralbibliothek |c 2013 | |
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Record in the Search Index
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| adam_text | CONTENT
CONTENT
1. SUMMARY 1
1.1 ZUSAMMENFASSUNG 2
2. INTRODUCTION 4
2.1 WHOLE-CELL BIOTRANSFORMATION 4
2.1.1 COFACTOR REGENERATION WITH SINGLE ENZYMES 4
2.1.2 COFACTOR REGENERATION VIA SUGAR METABOLISM 6
2.1.3 METABOLIC ENGINEERING OF THE PENTOSE PHOSPHATE PATHWAY 7
2.2 THE (R)-SPECIFIC ALCOHOL DEHYDROGENASE FROM LACTOBACILLUS BREVIS 8
2.3 BIOSENSORS 9
2.3.1 THE SOXRS SYSTEM 10
2.4 AIM OF THIS WORK 11
3. RESULTS 13
3.1 INCREASED NADPH AVAILABILITY IN ESCHERICHIA COLI 15
3.2 ENGINEERING YIELD AND RATE IN REDUCTIVE BIOTRANSFORMATION 25
3.3 CYCLIZATION OF THE PENTOSE PHOSPHATE PATHWAY IN C. GLUTAMICUM 35
3.4 CHARACTERIZATION OF E. COLI BL21(DE3) AND ITS PFKA DELETION MUTANT
46
3.5 BIOSENSOR FOR THE DETECTION OF LOW INTRACELLULAR NADPH/NADP
+
RATIOS 77
4. DISCUSSION 101
4.1 WHOLE-CELL BIOTRANSFORMATION 101
4.1.1 LINEAR FLUX THROUGH THE PPP IN ESCHERICHIA COLI 101
4.1.2 PARTIAL CYCLIZATION OF THE PPP IN ESCHERICHIA COLI 103
4.1.3 PARTIAL CYCLIZATION OF THE PPP IN CORYNEBACTERIUM GLUTAMICUM 104
4.1.4 CYCLIZATION OF THE PPP IN CORYNEBACTERIUM GLUTAMICUM 106
4.1.5 THE ROLE OF TRANSHYDROGENASE IN THE APFKA MUTANT OF E. COLI 107
4.2 NADPH/NADP
+
REDOX SENSOR 108
4.3 CONCLUSION AND PERSPECTIVES 111
REFERENCES 112
APPENDIX 120
SUPPLEMENTAL MATERIAL INCREASED NADPH AVAILABILITY IN ESCHERICHIA COLI
120
SUPPLEMENTAL MATERIAL CHARACTERIZATION OF . COLI BL21(DE3) AND ITS PFKA
DELETION MUTANT 123
HTTP://D-NB.INFO/1043821325
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| spelling | Siedler, Solvej Verfasser (DE-588)1042513929 aut Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler Jülich Forschungszentrum Jülich, Zentralbibliothek 2013 127 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Schriften des Forschungszentrums Jülich : Reihe Gesundheit 66 Zsfassung in dt. Sprache Zugl.: Düsseldorf, Univ., Diss., 2012 NADPH (DE-588)4171093-9 gnd rswk-swf Biotransformation (DE-588)4006908-4 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biosensor (DE-588)4193016-2 s NADPH (DE-588)4171093-9 s Biotransformation (DE-588)4006908-4 s DE-604 Schriften des Forschungszentrums Jülich Reihe Gesundheit ; 66 (DE-604)BV035800060 66 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027057064&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Siedler, Solvej Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Schriften des Forschungszentrums Jülich NADPH (DE-588)4171093-9 gnd Biotransformation (DE-588)4006908-4 gnd Biosensor (DE-588)4193016-2 gnd |
| subject_GND | (DE-588)4171093-9 (DE-588)4006908-4 (DE-588)4193016-2 (DE-588)4113937-9 |
| title | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_auth | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_exact_search | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_full | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler |
| title_fullStr | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler |
| title_full_unstemmed | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler |
| title_short | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_sort | increasing the nadph supply for whole cell biotransformation and development of a novel biosensor |
| topic | NADPH (DE-588)4171093-9 gnd Biotransformation (DE-588)4006908-4 gnd Biosensor (DE-588)4193016-2 gnd |
| topic_facet | NADPH Biotransformation Biosensor Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027057064&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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