Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Jülich
Forschungszentrum Jülich, Zentralbibliothek
2013
|
| Schriftenreihe: | Schriften des Forschungszentrums Jülich
Reihe Gesundheit ; 66 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | 127 S. Ill., graph. Darst. |
| ISBN: | 9783893369003 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV041615936 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 140131s2013 ad|| m||| 00||| eng d | ||
| 020 | |a 9783893369003 |9 978-3-89336-900-3 | ||
| 035 | |a (OCoLC)864653400 | ||
| 035 | |a (DE-599)GBV77673296X | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-12 | ||
| 082 | 0 | |a 500 | |
| 100 | 1 | |a Siedler, Solvej |e Verfasser |0 (DE-588)1042513929 |4 aut | |
| 245 | 1 | 0 | |a Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |c Solvej Siedler |
| 264 | 1 | |a Jülich |b Forschungszentrum Jülich, Zentralbibliothek |c 2013 | |
| 300 | |a 127 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Schriften des Forschungszentrums Jülich : Reihe Gesundheit |v 66 | |
| 500 | |a Zsfassung in dt. Sprache | ||
| 502 | |a Zugl.: Düsseldorf, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a NADPH |0 (DE-588)4171093-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biotransformation |0 (DE-588)4006908-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biosensor |0 (DE-588)4193016-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Biosensor |0 (DE-588)4193016-2 |D s |
| 689 | 0 | 1 | |a NADPH |0 (DE-588)4171093-9 |D s |
| 689 | 0 | 2 | |a Biotransformation |0 (DE-588)4006908-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 830 | 0 | |a Schriften des Forschungszentrums Jülich |v Reihe Gesundheit ; 66 |w (DE-604)BV035800060 |9 66 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027057064&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-027057064 | ||
Datensatz im Suchindex
| _version_ | 1804151823622209536 |
|---|---|
| adam_text | CONTENT
CONTENT
1. SUMMARY 1
1.1 ZUSAMMENFASSUNG 2
2. INTRODUCTION 4
2.1 WHOLE-CELL BIOTRANSFORMATION 4
2.1.1 COFACTOR REGENERATION WITH SINGLE ENZYMES 4
2.1.2 COFACTOR REGENERATION VIA SUGAR METABOLISM 6
2.1.3 METABOLIC ENGINEERING OF THE PENTOSE PHOSPHATE PATHWAY 7
2.2 THE (R)-SPECIFIC ALCOHOL DEHYDROGENASE FROM LACTOBACILLUS BREVIS 8
2.3 BIOSENSORS 9
2.3.1 THE SOXRS SYSTEM 10
2.4 AIM OF THIS WORK 11
3. RESULTS 13
3.1 INCREASED NADPH AVAILABILITY IN ESCHERICHIA COLI 15
3.2 ENGINEERING YIELD AND RATE IN REDUCTIVE BIOTRANSFORMATION 25
3.3 CYCLIZATION OF THE PENTOSE PHOSPHATE PATHWAY IN C. GLUTAMICUM 35
3.4 CHARACTERIZATION OF E. COLI BL21(DE3) AND ITS PFKA DELETION MUTANT
46
3.5 BIOSENSOR FOR THE DETECTION OF LOW INTRACELLULAR NADPH/NADP
+
RATIOS 77
4. DISCUSSION 101
4.1 WHOLE-CELL BIOTRANSFORMATION 101
4.1.1 LINEAR FLUX THROUGH THE PPP IN ESCHERICHIA COLI 101
4.1.2 PARTIAL CYCLIZATION OF THE PPP IN ESCHERICHIA COLI 103
4.1.3 PARTIAL CYCLIZATION OF THE PPP IN CORYNEBACTERIUM GLUTAMICUM 104
4.1.4 CYCLIZATION OF THE PPP IN CORYNEBACTERIUM GLUTAMICUM 106
4.1.5 THE ROLE OF TRANSHYDROGENASE IN THE APFKA MUTANT OF E. COLI 107
4.2 NADPH/NADP
+
REDOX SENSOR 108
4.3 CONCLUSION AND PERSPECTIVES 111
REFERENCES 112
APPENDIX 120
SUPPLEMENTAL MATERIAL INCREASED NADPH AVAILABILITY IN ESCHERICHIA COLI
120
SUPPLEMENTAL MATERIAL CHARACTERIZATION OF . COLI BL21(DE3) AND ITS PFKA
DELETION MUTANT 123
HTTP://D-NB.INFO/1043821325
|
| any_adam_object | 1 |
| author | Siedler, Solvej |
| author_GND | (DE-588)1042513929 |
| author_facet | Siedler, Solvej |
| author_role | aut |
| author_sort | Siedler, Solvej |
| author_variant | s s ss |
| building | Verbundindex |
| bvnumber | BV041615936 |
| ctrlnum | (OCoLC)864653400 (DE-599)GBV77673296X |
| dewey-full | 500 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 500 - Natural sciences and mathematics |
| dewey-raw | 500 |
| dewey-search | 500 |
| dewey-sort | 3500 |
| dewey-tens | 500 - Natural sciences and mathematics |
| discipline | Allgemeine Naturwissenschaft |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01782nam a2200421 cb4500</leader><controlfield tag="001">BV041615936</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">140131s2013 ad|| m||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783893369003</subfield><subfield code="9">978-3-89336-900-3</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)864653400</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBV77673296X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">500</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Siedler, Solvej</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1042513929</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor</subfield><subfield code="c">Solvej Siedler</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Jülich</subfield><subfield code="b">Forschungszentrum Jülich, Zentralbibliothek</subfield><subfield code="c">2013</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">127 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Schriften des Forschungszentrums Jülich : Reihe Gesundheit</subfield><subfield code="v">66</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Zsfassung in dt. Sprache</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Zugl.: Düsseldorf, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">NADPH</subfield><subfield code="0">(DE-588)4171093-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biotransformation</subfield><subfield code="0">(DE-588)4006908-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biosensor</subfield><subfield code="0">(DE-588)4193016-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Biosensor</subfield><subfield code="0">(DE-588)4193016-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">NADPH</subfield><subfield code="0">(DE-588)4171093-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Biotransformation</subfield><subfield code="0">(DE-588)4006908-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Schriften des Forschungszentrums Jülich</subfield><subfield code="v">Reihe Gesundheit ; 66</subfield><subfield code="w">(DE-604)BV035800060</subfield><subfield code="9">66</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027057064&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-027057064</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV041615936 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:00:57Z |
| institution | BVB |
| isbn | 9783893369003 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027057064 |
| oclc_num | 864653400 |
| open_access_boolean | |
| owner | DE-12 |
| owner_facet | DE-12 |
| physical | 127 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Forschungszentrum Jülich, Zentralbibliothek |
| record_format | marc |
| series | Schriften des Forschungszentrums Jülich |
| series2 | Schriften des Forschungszentrums Jülich : Reihe Gesundheit |
| spelling | Siedler, Solvej Verfasser (DE-588)1042513929 aut Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler Jülich Forschungszentrum Jülich, Zentralbibliothek 2013 127 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Schriften des Forschungszentrums Jülich : Reihe Gesundheit 66 Zsfassung in dt. Sprache Zugl.: Düsseldorf, Univ., Diss., 2012 NADPH (DE-588)4171093-9 gnd rswk-swf Biotransformation (DE-588)4006908-4 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biosensor (DE-588)4193016-2 s NADPH (DE-588)4171093-9 s Biotransformation (DE-588)4006908-4 s DE-604 Schriften des Forschungszentrums Jülich Reihe Gesundheit ; 66 (DE-604)BV035800060 66 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027057064&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Siedler, Solvej Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Schriften des Forschungszentrums Jülich NADPH (DE-588)4171093-9 gnd Biotransformation (DE-588)4006908-4 gnd Biosensor (DE-588)4193016-2 gnd |
| subject_GND | (DE-588)4171093-9 (DE-588)4006908-4 (DE-588)4193016-2 (DE-588)4113937-9 |
| title | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_auth | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_exact_search | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_full | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler |
| title_fullStr | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler |
| title_full_unstemmed | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor Solvej Siedler |
| title_short | Increasing the NADPH supply for whole-cell biotransformation and development of a novel biosensor |
| title_sort | increasing the nadph supply for whole cell biotransformation and development of a novel biosensor |
| topic | NADPH (DE-588)4171093-9 gnd Biotransformation (DE-588)4006908-4 gnd Biosensor (DE-588)4193016-2 gnd |
| topic_facet | NADPH Biotransformation Biosensor Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027057064&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035800060 |
| work_keys_str_mv | AT siedlersolvej increasingthenadphsupplyforwholecellbiotransformationanddevelopmentofanovelbiosensor |