Functional analysis of DNA and chromatin:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1094 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 365 S. Ill., graph. Darst. |
| ISBN: | 9781627037051 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804151780110499840 |
|---|---|
| adam_text | Titel: Functional analysis of DNA and chromatin
Autor: Stockert, Juan C
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... xiii
1 Predictive Binding Geometry of Ligands to DNA Minor Groove:
Isohelicity and Hydrogen-Bonding Pattern........................... 1
Juan C. Stockert
2 Using Microchip Gel Electrophoresis to Probe DNA-Drug
Binding Interactions............................................ 13
Nan Shi and Victor M. Ugaz
3 Identifying Different Types of Chromatin Using Giemsa Staining.......... 25
Juan C. Stockert, Alfonso Blázquez-Castro, and Richard W. Horobin
4 Analysis of DNA Damage and Repair by Comet Fluorescence
In Situ Hybridization (Comet-FISH)............................... 39
Michael Glei and Wiebke Schlörmann
5 Alkaline Nuclear Dispersion Assays for the Determination
of DNA Damage at the Single Cell Level............................. 49
Piero Sestili and Carmela Fimognari
6 Polarization Microscopy of Extended Chromatin Fibers................. 71
Maria Luiza S. Mello and Benedicto de Campos Vidal
7 DNA Labeling In Vivo: Quantification of Epidermal Stem Cell
Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image
Processing Software............................................ 79
Elisa Carrasco, María I. Calvo, and Jesús Espada
8 A Historical Overview of Bromo-Substituted DNA
and Sister Chromatid Differentiation................................ 89
Roberto Mezzanotte and Mariella Nieddu
9 Image Analysis of Chromatin Remodelling........................... 99
Benedicto de Campos Vidal, Marina B. Eelisbino, and Maria Luiza S. Mello
10 FISH Methods in Cytogenetic Studies.............................. 109
Miguel Pita, Juan Orellana, Paloma Martínez-Rodríguez,
Ángel Martínez-Ramírez, Begoña Ferndndez-Calvín, and José L. Bella
11 Ultrastructural and Immunofluorescent Methods for the Study
of the XY Body as a Biomarker.................................... 137
Roberta B. Sciurano and Alberto J. Solari
¡X
x Contents
12 Atomic Force Microscopy for Analyzing Metaphase Chromosomes:
Comparison of AFM Images with Fluorescence Labeling Images
of Banding Patterns............................................ 151
Osamu Hoshi and Tatsuo Ushiki
13 Selective Detection of Phagocytic Phase of Apoptosis
in Fixed Tissue Sections......................................... 159
Vladimir V. Didenko
14 Selective Transport of Canonized Fluorescent Topoisomerase into Nuclei
of Live Cells for DNA Damage Studies.............................. 167
Candace L. Minchew and Vladimir V. Didenko
15 Visualization and Interpretation of Eukaryotic DNA Replication
Intermediates In Vivo by Electron Microscopy........................ 177
Kai J. Keelsen, Arnab Ray Chaudhuri, Cindy Follonier, Raquel Herrador,
and Massimo Lopes
16 Combined Bidimensional Electrophoresis and Electron Microscopy
to Study Specific Plasmid DNA Replication Intermediates in Human Cells . . . 209
Cindy Follonier and Massimo Lopes
17 Standard DNA Methylation Analysis in Mouse Epidermis:
Bisulfite Sequencing, Methylation-Specific PCR,
and 5-Methyl-Cytosine (5mC) Immunological Detection................ 221
Jesús Espada, Elisa Carrasco, and María I. Calvo
18 Methyl-Combing: Single-Molecule Analysis of DNA Methylation
on Stretched DNA Fibers........................................ 233
Attila Németh
19 Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art....... 243
Colm E. Nestor, James P. Reddington, Mikael Benson,
and Richard R. Meehan
20 Hydroxymethylated DNA Immunoprecipitation (hmeDIP)............... 259
Colm E. Nestor and Richard R Meehan
21 Microscale Thermophoresis for the Assessment
of Nuclear Protein-Binding Affinities............................... 269
Wei Zhang, Stefan Duhr, Philipp Baaske, and Ernest Laue
22 Analysis of Histone Posttranslational Modifications
from Nucleolus-Associated Chromatin by Mass Spectrometry............. 277
Stefan Dillinger, Ana Villar Garea, Rainer Deutsmann,
and Attila Németh
23 Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography
Methods for Histone Isolation and Fractionation...................... 295
Pedro Rodriguez-Collazo, Sanford Leuba, Eduardo Karahanian, and
Jordanka Zlatanova
24 Chromatin Immunoprecipitation.................................. 309
Javier Rodriguez-Ubreva and Esteban Ballestar
25 Analysis of Chromatin Composition of Repetitive Sequences:
The ChlP-Chop Assay.......................................... 319
Raffaella Santoro
Contents xi
26 Purification of Specific Chromatin Domains
from Single-Copy Gene Loci in Saccharomyces cerevisiae................. 329
Stephan Hamperl, Christopher R. Brown, Jorge Perez-Fernandez,
Katharina Huber, Manuel Wittner, Virginia Babl, Ulrike Stöckl,
Hinrich Boeger, Herbert Tschochner, Philipp Milkereit,
and Joachim Griesenbeck
27 Deep Sequencing of Small Chromatin-Associated RNA: Isolation
and Library Preparation......................................... 343
Sarah Daniela Diermeier, Thomas Schubert, and Gemot Längst
28 Deep Sequencing of Small Chromatin-Associated RNA:
Bioinformatic Analysis........................................... 355
Sarah Daniela Diermeier and Gemot Längst
Index............................................................... 361
|
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| illustrated | Illustrated |
| indexdate | 2024-07-10T01:00:15Z |
| institution | BVB |
| isbn | 9781627037051 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027033043 |
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| physical | XV, 365 S. Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Functional analysis of DNA and chromatin ed. by Juan C. Stockert ... New York [u.a.] Humana Press 2014 XV, 365 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1094 Springer protocols Genanalyse (DE-588)4200230-8 gnd rswk-swf Chromosomenanalyse (DE-588)4147988-9 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf Analyse (DE-588)4122795-5 gnd rswk-swf DNS (DE-588)4070512-2 s Analyse (DE-588)4122795-5 s DE-604 Genanalyse (DE-588)4200230-8 s Chromosomenanalyse (DE-588)4147988-9 s Stockert, Juan C. Sonstige oth Erscheint auch als Online-Ausgabe Functional Analysis of DNA and Chromatin Methods in molecular biology 1094 (DE-604)BV035362695 1094 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027033043&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Functional analysis of DNA and chromatin Methods in molecular biology Genanalyse (DE-588)4200230-8 gnd Chromosomenanalyse (DE-588)4147988-9 gnd DNS (DE-588)4070512-2 gnd Analyse (DE-588)4122795-5 gnd |
| subject_GND | (DE-588)4200230-8 (DE-588)4147988-9 (DE-588)4070512-2 (DE-588)4122795-5 |
| title | Functional analysis of DNA and chromatin |
| title_auth | Functional analysis of DNA and chromatin |
| title_exact_search | Functional analysis of DNA and chromatin |
| title_full | Functional analysis of DNA and chromatin ed. by Juan C. Stockert ... |
| title_fullStr | Functional analysis of DNA and chromatin ed. by Juan C. Stockert ... |
| title_full_unstemmed | Functional analysis of DNA and chromatin ed. by Juan C. Stockert ... |
| title_short | Functional analysis of DNA and chromatin |
| title_sort | functional analysis of dna and chromatin |
| topic | Genanalyse (DE-588)4200230-8 gnd Chromosomenanalyse (DE-588)4147988-9 gnd DNS (DE-588)4070512-2 gnd Analyse (DE-588)4122795-5 gnd |
| topic_facet | Genanalyse Chromosomenanalyse DNS Analyse |
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