DNA electrophoresis: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2013
|
| Schriftenreihe: | Methods in molecular biology
1054 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 299 S. Ill., graph. Darst. |
| ISBN: | 9781627035644 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804151779951116288 |
|---|---|
| adam_text | Titel: DNA electrophoresis
Autor: Makovets, Svetlana
Jahr: 2013
Contents
Preface.............................................................. v
Contributors.......................................................... ix
1 DNA Electrophoresis: Historical and Theoretical Perspectives............. 1
Gar et h A. Roberts and David T.F. Dryden
2 Basic DNA Electrophoresis in Molecular Cloning: A Comprehensive
Guide for Beginners............................................ 11
Svetlana Makovets
3 Analysis of Branched DNA Replication and Recombination Intermediates
from Prokaryotic Cells by Two-Dimensional (2D) Native-Native Agarose
Gel Electrophoresis............................................. 45
Nicholas P. Robinson
4 Analysis of DNA Structures from Eukaryotic Cells by Two-Dimensional
Native-Native DNA Agarose Gel Electrophoresis...................... 63
Andreas S. Ivessa
5 Directionality of Replication Fork Movement Determined
by Two-Dimensional Native-Native DNA Agarose Gel Electrophoresis...... 83
Andreas S. Ivessa
6 Native/Denaturing Two-Dimensional DNA Electrophoresis
and Its Application to the Analysis of Recombination Intermediates........ 105
Jessica P. Lao, Shangming Tang, and Neil Hunter
7 Plasmid DNA Topology Assayed by Two-Dimensional Agarose
Gel Electrophoresis............................................. 121
Jorge B. Schvartzman, Marta-Luisa Martinez-Robles,
Pablo Hernandez, and Dora B. Krimer
8 A Neutral Glyoxal Gel Electrophoresis Method for the Detection
and Semi-quantitation of DNA Single-Strand Breaks.................... 133
Brian Pachkowski and Jun Nakamura
9 Denaturing Gradient Gel Electrophoresis (DGGE)..................... 145
Fiona Strathdee and Andrew Free
10 Polyacrylamide Temperature Gradient Gel Electrophoresis............... 159
Viktor Viglasky
11 Separation of DNA Oligonucleotides Using Denaturing Urea PAGE........ 173
Fiona Flett and Heidrun Interthal
12 Pulsed-Field Gel Electrophoresis of Bacterial Chromosomes.............. 187
Julia S.P. Mawer and David R.F. Leach
vii
viii
Contents
13 Resolution of Budding Yeast Chromosomes Using Pulsed-Field
Gel Electrophoresis............................................. 195
Aziz El Hage and Jonathan Houseley
14 Analysis of DNA Damage via Single-Cell Electrophoresis................ 209
Diana Anderson and Julian Laubenthal
15 Fluorescence In Situ Hybridization on Electrophoresed Cells
to Detect Sequence Specific DNA Damage........................... 219
Julian Laubenthal and Diana Anderson
16 Analysis of DNA-Protein Interactions Using PAGE: Band-Shift Assays...... 237
Lynn Powell
17 Assaying Cooperativity of Protein-DNA Interactions Using Agarose
Gel Electrophoresis............................................. 253
Tanya L. Williams and Daniel L. Levy
18 DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool.......... 267
Fenfei Leng
19 Using PCR Coupled to PAGE for Detection and Semiquantitative
Evaluation of Telomerase Activity.................................. 283
Laura Gardano
Index............................................................... 295
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| spelling | DNA electrophoresis methods and protocols ed. by Svetlana Makovets New York [u.a.] Humana Press 2013 X, 299 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1054 Springer protocols Makovets, Svetlana edt Methods in molecular biology 1054 (DE-604)BV035362695 1054 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027032935&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA electrophoresis methods and protocols Methods in molecular biology |
| title | DNA electrophoresis methods and protocols |
| title_auth | DNA electrophoresis methods and protocols |
| title_exact_search | DNA electrophoresis methods and protocols |
| title_full | DNA electrophoresis methods and protocols ed. by Svetlana Makovets |
| title_fullStr | DNA electrophoresis methods and protocols ed. by Svetlana Makovets |
| title_full_unstemmed | DNA electrophoresis methods and protocols ed. by Svetlana Makovets |
| title_short | DNA electrophoresis |
| title_sort | dna electrophoresis methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027032935&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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