Bioluminescent imaging: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1098 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 276 S. Ill., graph. Darst. |
| ISBN: | 9781627037174 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804151769115131904 |
|---|---|
| adam_text | Titel: Bioluminescent imaging
Autor: Badr, Christian E
Jahr: 2014
Contents
Preface.......................................................... v
Contributors.......................................................... ix
1 Bioluminescence Imaging: Basics and Practical Limitations............... 1
Christian E. Badr
Part I In Vitro Bioassays
2 Extraction and Quantification of Adenosine Triphosphate
in Mammalian Tissues and Cells................................... 21
Junji Chida and Hiroshi Kido
3 Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive
Bioluminescent Reporter......................................... 33
Ludovic Tricoire and Bertrand Lambolez
4 Gaussia Luciferase-Based Mycoplasma Detection Assay
in Mammalian Cell Culture....................................... 47
M. Hannah Degeling, M. Sarah S. Bovenberg, Marie Tannous,
and Bakhos A. Tannous
Part II Imaging Molecular and Cellular Events
5 Split Gaussia Luciferase for Imaging Ligand-Receptor Binding............ 59
Kathryn E. Luker and Gary D. Luker
6 Video-Rate Bioluminescence Imaging of Protein Secretion
from a Living Cell.............................................. 71
Takahiro Suzuki and Satoshi Inouye
7 Bioluminescence Reporter Gene-Based Detection of MicroRNAs.......... 85
Hae Young Ko, Young Sik Lee, and Soonhag Kim
8 Monitoring of Transcriptional Dynamics of HIF and NFkB Activities....... 97
Miguel A.S. Cavadas and Alex Cheong
9 Real-Time Bioluminescent Tracking of Cellular Population Dynamics....... 107
Dan Close, TingtingXu, Steven Ripp, and Gary Sayler
10 Fabrication of Bioluminescent Capsules and Live-Cell Imaging............ 117
Sung Bae Kim
Part III Imaging of Disease (Pathogenesis)
11 The Bioluminescent Imaging of Spontaneously Occurring Tumors
in Immunocompetent ODD-Luciferase Bearing Transgenic Mice........... 129
Scott J. Goldman and Shengkan Jin
vii
viii
Contents
12 Blood-Based Assay with Secreted Gaussia Luciferase
to Monitor Tumor Metastasis..................................... 145
Hiroshi Tamashita, Dan T. Nguyen, and Euiheon Chung
13 Bioluminescence Imaging of Fungal Biofilm Development in Live Animals ... 153
Greetje Vande Velde, Soda KucharikovA, Patrick Van Dijck,
and Uwe Himmelreich
14 Bioluminescent Imaging of Bacteria During Mouse Infection............. 169
Jonathan M. Warawa and Matthew B. Lawrenz
Part IV Therapeutic Imaging
15 Cell-Based Bioluminescence Screening Assays......................... 185
Romain J. Amante and Christian E. Badr
16 Bioluminescence-Based Monitoring of Virus Vector-Mediated
Gene Transfer in Mice........................................... 197
Casey A. Maguire
17 Simultaneous In Vivo Monitoring of Regulatory and Effector
T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase,
and Secreted Alkaline Phosphatase................................. 211
Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar,
BakhosA. Tannous, and Jamil Azzi
Part V Emerging Technologies
18 Multiplex Functional Bioluminescent Reporters Using Gaussia
Luciferase Fused to Epitope Tags in an Immunobinding Assay............ 231
Sjoerd van Rijn, Thomas Würdinger, and Jonas Nilsson
19 Noninvasive In Vivo Monitoring of Extracellular Vesicles................ 249
Charles P. Lai, BakhosA. Tannous, andXandra O. Breakefield
20 In Vitro and In Vivo Demonstrations of Fluorescence
by Unbound Excitation from Luminescence (FUEL)................... 259
Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson,
Samantha Blazquez, Kelly L. Rogers, Régis Tournebize, and Spencer Shorte
Index............................................................... 271
|
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| id | DE-604.BV041580757 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T01:00:05Z |
| institution | BVB |
| isbn | 9781627037174 |
| language | English |
| lccn | 2013950898 |
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| physical | XI, 276 S. Ill., graph. Darst. |
| publishDate | 2014 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Bioluminescent imaging methods and protocols ed. by Christian E. Badr New York [u.a.] Humana Press 2014 XI, 276 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1098 Springer protocols Badr, Christian E. (DE-588)1049206029 edt Methods in molecular biology 1098 (DE-604)BV035362695 1098 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027026018&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bioluminescent imaging methods and protocols Methods in molecular biology |
| title | Bioluminescent imaging methods and protocols |
| title_auth | Bioluminescent imaging methods and protocols |
| title_exact_search | Bioluminescent imaging methods and protocols |
| title_full | Bioluminescent imaging methods and protocols ed. by Christian E. Badr |
| title_fullStr | Bioluminescent imaging methods and protocols ed. by Christian E. Badr |
| title_full_unstemmed | Bioluminescent imaging methods and protocols ed. by Christian E. Badr |
| title_short | Bioluminescent imaging |
| title_sort | bioluminescent imaging methods and protocols |
| title_sub | methods and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027026018&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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