DNA cloning and assembly methods:
Gespeichert in:
Weitere Verfasser: | |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
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Schriftenreihe: | Methods in molecular biology
1116 |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XI, 303 S. Ill. |
ISBN: | 9781627037631 |
Internformat
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Datensatz im Suchindex
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adam_text | Titel: DNA cloning and assembly methods
Autor: Valla, Svein
Jahr: 2014
Contents
Preface............................................................................................................................. v
Contributors.................................................................................................................... ix
1 BioBrick Assembly Standards and Techniques
and Associated Software Tools.......................................................................... 1
Gunvor R0kke, Eirin Korvald, Jarle Pahr, Ove 0yâs, and Rahmt Laie
2 Plasmid Construction by SLIC or Sequence
and Ligation-Independent Cloning.................................................................. 25
Ryan E. Hill and Julian J. Eaton-Rye
3 Quick and Clean Cloning................................................................................. 37
Frank Thieme and Sylvestre Marillonnet
4 Hierarchical Ligation-Independent Assembly of PCR Fragments...................... 49
Jonathan L. Schmid-Burgk, Zhen Xie, and Taakov Benenson
5 USER-Derived Cloning Methods and Their Primer Design.............................. 59
Bo Salomonsen, Ujfe H. Mortensen, and Barbara A. Halkier
6 Application of the Restriction-Free (RF) Cloning
for Multicomponents Assembly........................................................................ 73
Yoav Peleg and Tamar Unger
7 A Single-Tube Assembly of DNA Using the
Transfer-PCR (TPCR) Platform....................................................................... 89
Ariel Erijman, Julia M. Shifinan, and Yoav Peleg
8 Circular Polymerase Extension Cloning............................................................ 103
Jiayuan Qjtan and Jingdong Tian
9 Golden Gate Cloning....................................................................................... 119
Carola Engler and Sylvestre Marillonnet
10 Design and Construction of Multigenic Constructs
for Plant Biotechnology Using the GoldenBraid Cloning Strategy.................... 133
Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell,
and Diego Orzaez
11 FX Cloning: A Simple and Robust High-Throughput Cloning
Method for Protein Expression......................................................................... 153
Eric R. Geertsma
12 Minimum GC-Rich Sequences for Overlap Extension PCR
and Primer Annealing....................................................................................... 165
Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida,
and Rinji Akada
vii
viii
Contents
13 Simple Cloning and DNA Assembly in Escherichia coli
by Prolonged Overlap Extension PCR.............................................................. 183
Chun Tou and T.-H. Percival Zhang
14 Combinatorial Assembly of Clone Libraries Using
Site-Specific Recombination............................................................................. 193
Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko,
Lauren V. Procter, and Dominic Esposito
15 Application of In-Fusion™ Cloning for the Parallel Construction
of E. coli Expression Vectors............................................................................. 209
Louise E. Bird, Heather Rada,John Flanagan, Jonathan M. Diprose,
Robert J.C. Gilbert, and Raymond J. Owens
16 Seamless Ligation Cloning Extract (SLiCE) Cloning Method........................... 235
Yongwei Zhang, Uwe Werling, and Winfried Edelmann
17 ) 5 DNA Assembly Design Automation.............................................................. 245
Nathan J. Hillson
18 FastPCR Software for PCR, In Silico PCR, and Oligonucleotide
Assembly and Analysis...................................................................................... 271
Ruslan Kalendar, David Lee, and Alan H. Schulman
Index............................................................................................................................... 303
|
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isbn | 9781627037631 |
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spelling | DNA cloning and assembly methods Ed. by Svein Valla... New York, NY [u.a.] Humana Press 2014 XI, 303 S. Ill. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1116 Springer protocols Valla, Svein edt Methods in molecular biology 1116 (DE-604)BV035362695 1116 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027010784&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | DNA cloning and assembly methods Methods in molecular biology |
title | DNA cloning and assembly methods |
title_auth | DNA cloning and assembly methods |
title_exact_search | DNA cloning and assembly methods |
title_full | DNA cloning and assembly methods Ed. by Svein Valla... |
title_fullStr | DNA cloning and assembly methods Ed. by Svein Valla... |
title_full_unstemmed | DNA cloning and assembly methods Ed. by Svein Valla... |
title_short | DNA cloning and assembly methods |
title_sort | dna cloning and assembly methods |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027010784&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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