Characterization of WUSCHEL enhancers in Arabidopsis thaliana:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 105 Bl. Ill., graph. Darst. 30 cm |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
TABLE OF CONTENTS 4
ABSTRACT 6
INTRODUCTION 7
EMBRYONIC INITIATION OF THE SHOOT MERISTEM 7
REGULATORY NETWORKS IN THE POST-EMBRYONIC SAM 12
AIMS OF THE STUDY 18
RESULTS 19
PART I: A MUTAGENESIS
SCREEN FOR ENHANCERS
OF THE WEAK WUSCHEL ALLELE
WUS-7
19
1.1
STRATEGY FOR THE IDENTIFICATION OF SECOND-SITE MODIFIERS OF THE WUS-7
PHENOTYPE 19
1.2 F197 IS A
NEW ALLELE OF WUS 21
1.2.1
IDENTIFICATION OF F197 AS A NEW WUS ALLELE. 21
1.3 THREE ENHANCER CANDIDATES ARE NOVEL ALLELES OF REVOLUTA 22
1.3.1
C396 CO-SEGREGATES A SINGLE MUTANT PHENOTYPE 22
1.3.2 P100 CO-SEGREGATES
A SINGLE MUTANT PHENOTYPE 24
1.3.3 C97 WAS ISOLATED
AS AN ENHANCER HOMOZYGOUS IN THE M2 26
1.3.4 BULK MAPPING
OF C396, P100 AND C97 SHOWS LINKAGE TO CHROMOSOME 5 28
1.3.5 GENETIC COMPLEMENTATION OF C396, P100 AND C97 TO REV-6 30
1.3.6 C396, P100 AND C97 ARE NEW ALLELES OF REV 32
1.4 OTHER ALLELES ISOLATED IN THE SCREEN 33
1.4.1
ENHANCER M94 33
1.4.2 ENHANCER C55 36
1.4.3 ENHANCER T67 41
1.4.4 ENHANCER T22 43
1.4.5 ENHANCER K42 44
1.5 SUMMARY OF THE ENHANCER SCREEN 46
PART II:
FUNCTIONAL ANALYSIS OF REV IN THE WUS PATHWAY 47
11.1 WUS
IS NOT DIRECTLY REGULATED BY REV 47
11.1.1 WUS
TRANSCRIPTION IS NOT SIGNIFICANTLY CHANGED IN REV MUTANTS 47
11.1.2 WUS AND CLV3 EXPRESSION
ARE UNCHANGED IN REV-LOD 48
11.1.3 THE REV-LOD
WUS-1 FLOWER PHENOTYPE IS NOT CAUSED BY AG MISEXPRESSION 50
11.2 WUS AND REV CONVERGE ON COMMON TARGET GENES 52
11.2.1 CLV3 AND
ARR7 ARE COMMON TARGET GENES OF REV AND WUS 52
11.2.2 DOUBLE OVEREXPRESSION OF WUS
AND REV INDUCES EXTRA STEM CELLS 53
11.3 WUS MOVEMENT IS NOT ALTERED IN A REV-6 BACKGROUND 55
PART III
FLUORESCENT MARKER EXRESSION IN
THE EMBRYONIC
SHOOT MERISTEM 58
111.1 WUS AND CLV3 58
111.2 CLV3, UFO AND ZLL 60
DISCUSSION 63
I THE SECOND-SITE MODIFIER SCREEN AS A
RESEARCH TOOL TO FIND WUS INTERACTION PARTNERS 63
1.1
STRATEGY OF THE ENHANCER SCREEN 63
1.2 A NEW ALLELE OF WUS ISOLATED IN THE SCREEN VALIDATES THE SCREENING
APPROACH 64
1.3 THREE ALLELES
OF THE HD-ZIP III TRANSCRIPTION FACTOR REV WERE ISOLATED IN THE SCREEN
64
II REV AS A WUS ENHANCER 66
LL.L REV ENHANCES WUS-7 BUT NOT WUS-1 SEEDLINGS 66
11.3 REV AND WUS SIGNALING CONVERGES
ON ARR7 AND CLV3 67
11.3 REV MILDLY ENHANCES THE
CAPABILITY FOR WUS SIGNALING 70
11.4 MODEL FOR THE SUGGESTED INTERACTION BETWEEN REV AND WUS 71
HTTP://D-NB.INFO/1044568194
ILL FLUORESCENT
MARKER EXPRESSION IN THE EMBRYONIC SHOOT MERISTEM 71
111.1 SEPARATION OF THE
ORGANIZING CENTER AND STEM CELL DOMAINS 71
111.2 THE USE OF MULTIPLE MARKER LINES TO STUDY ARABIDOPSIS
EMBRYOGENESIS 72
MATERIALS AND METHODS 74
MATERIALS 74
METHODS 76
ABBREVIATIONS 85
REFERENCES 87
APPENDICES 94
APPENDIX 1: PHENOTYPE NOMECLATURE 94
APPENDIX 2: PLASMIDS
GENERATED FOR THIS STUDY 95
APPENDIX 3: PLASMIDS
FROM OTHER SOURCES USED IN THIS
STUDY 95
APPENDIX 4. TRANSGENIC LINES ANALYZED
IN THIS STUDY 96
APPENDIX 5: OLIGONUCLEOTIDES GENERATED FOR THIS STUDY 97
APPENDIX 6: OLIGONUCLEOTIDE SEQUENCES
FOR BULK MAPPING 99
APPENDIX 7: OLIGONUCLEOTIDE
SEQUENCES USED FOR QPCR ; 101
APPENDIX 8: MATERIALS USED FOR EMSA EXPERIMENTS 102
APPENDIX 9: OLIGONUCLEOTIDES
USED FOR CHIP EXPERIMENTS 104
ACKNOWLEDGEMENTS 105
5
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| any_adam_object | 1 |
| author | Haperen, Johanna Maria Adriana van |
| author_GND | (DE-588)1043006508 |
| author_facet | Haperen, Johanna Maria Adriana van |
| author_role | aut |
| author_sort | Haperen, Johanna Maria Adriana van |
| author_variant | j m a v h jmav jmavh |
| building | Verbundindex |
| bvnumber | BV041564768 |
| ctrlnum | (OCoLC)865732814 (DE-599)DNB1044568194 |
| dewey-full | 575.482364 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 575 - Specific parts of & systems in plants |
| dewey-raw | 575.482364 |
| dewey-search | 575.482364 |
| dewey-sort | 3575.482364 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| spelling | Haperen, Johanna Maria Adriana van Verfasser (DE-588)1043006508 aut Characterization of WUSCHEL enhancers in Arabidopsis thaliana vorgelegt von Johanna Maria Adriana van Haperen 2013 105 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027010300&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Haperen, Johanna Maria Adriana van Characterization of WUSCHEL enhancers in Arabidopsis thaliana |
| subject_GND | (DE-588)4113937-9 |
| title | Characterization of WUSCHEL enhancers in Arabidopsis thaliana |
| title_auth | Characterization of WUSCHEL enhancers in Arabidopsis thaliana |
| title_exact_search | Characterization of WUSCHEL enhancers in Arabidopsis thaliana |
| title_full | Characterization of WUSCHEL enhancers in Arabidopsis thaliana vorgelegt von Johanna Maria Adriana van Haperen |
| title_fullStr | Characterization of WUSCHEL enhancers in Arabidopsis thaliana vorgelegt von Johanna Maria Adriana van Haperen |
| title_full_unstemmed | Characterization of WUSCHEL enhancers in Arabidopsis thaliana vorgelegt von Johanna Maria Adriana van Haperen |
| title_short | Characterization of WUSCHEL enhancers in Arabidopsis thaliana |
| title_sort | characterization of wuschel enhancers in arabidopsis thaliana |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027010300&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT haperenjohannamariaadrianavan characterizationofwuschelenhancersinarabidopsisthaliana |