miRNA maturation: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2014
|
| Schriftenreihe: | Methods in molecular biology
1095 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 192 S. Ill., graph. Darst. |
| ISBN: | 9781627037020 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804151746482667520 |
|---|---|
| adam_text | Titel: MiRNA maturation
Autor: Arenz, Christoph
Jahr: 2014
Contents
Preface.............................................................. v
Contributors.......................................................... ix
Part I Reviews
1 The Pathway of miRNA Maturation................................ 3
Michael Sand
2 MicroRNA Maturation and Human Disease.......................... 11
Marlen Hesse and Christoph Arenz
3 Approaches to the Modulation of miRNA Maturation................... 27
Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young
Part II Methods
4 Expression Profiling of Components of the miRNA
Maturation Machinery.......................................... 61
Michael Sand and Marina Skrygan
5 Primary MicroRNA Processing Assay Reconstituted
Using Recombinant Drosha and DGCR8............................ 73
Ian Barr and Feng Guo
6 In Vivo Processing Assay Based on a Dual-Luciferase
Reporter System to Evaluate DROSHA Enzymatic Activity............... 87
Vera Bilan, Danilo Allegra, Florian Kuchenbauer, and Daniel Mertens
7 Assaying Dicer-Mediated miRNA Maturation
by Means of Fluorescent Substrates................................. 95
Marlen Hesse, Brian P. Davies, and Christoph Arenz
8 A Fluorescence Correlation Spectroscopy-Based Enzyme Assay
for Human Dicer.............................................. 103
Eileen Magbanua and Ulrich Hahn
9 Detection of microRNA Maturation Using Unmodified
pre-microRNA and Branched Rolling Circle Amplification............... 109
Saskia Neubacher and Christoph Arenz
10 Quantitative RT-PCR Specific for Precursor
and Mature miRNAs............................................ 121
Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj
vii
viii
Contents
11 Cellular MicroRNA Sensors Based on Luciferase Reporters............... 135
Colleen M. Connelly and Alexander Deiters
12 Identification of Inhibitors of MicroRNA Function
from Small Molecule Screens...................................... 147
Colleen M. Connelly and Alexander Deiters
13 Inhibition of miRNA Maturation by Peptide Nucleic Acids............... 157
Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari,
and Alessandra Romanelli
14 Molecular Methods for Validation of the Biological Activity
of Peptide Nucleic Acids Targeting MicroRNAs....................... 165
Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti,
Roberto Corradini, and Roberto Gambari
15 Lentiviral Overexpression of miRNAs............................... 177
Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Majjhnouj
Index............................................................... 191
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| id | DE-604.BV041564452 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:59:43Z |
| institution | BVB |
| isbn | 9781627037020 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-027009989 |
| oclc_num | 868355944 |
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| physical | X, 192 S. Ill., graph. Darst. |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | miRNA maturation methods and protocols ed. by Christoph Arenz New York, NY [u.a.] Humana Press 2014 X, 192 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1095 Springer protocols miRNS (DE-588)7736808-3 gnd rswk-swf miRNS (DE-588)7736808-3 s DE-604 Arenz, Christoph 1971- (DE-588)123014549 edt Erscheint auch als Online-Ausgabe miRNA Maturation Methods in molecular biology 1095 (DE-604)BV035362695 1095 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027009989&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | miRNA maturation methods and protocols Methods in molecular biology miRNS (DE-588)7736808-3 gnd |
| subject_GND | (DE-588)7736808-3 |
| title | miRNA maturation methods and protocols |
| title_auth | miRNA maturation methods and protocols |
| title_exact_search | miRNA maturation methods and protocols |
| title_full | miRNA maturation methods and protocols ed. by Christoph Arenz |
| title_fullStr | miRNA maturation methods and protocols ed. by Christoph Arenz |
| title_full_unstemmed | miRNA maturation methods and protocols ed. by Christoph Arenz |
| title_short | miRNA maturation |
| title_sort | mirna maturation methods and protocols |
| title_sub | methods and protocols |
| topic | miRNS (DE-588)7736808-3 gnd |
| topic_facet | miRNS |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027009989&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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