Urea transport in sheep rumen epithelium in vitro: Modulation by luminal Ammonia and pH
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Untersuchungen über den Transport von Harnstoff des ovinen Pansenepithels |
| Beschreibung: | 114 Bl. graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a Urea transport in sheep rumen epithelium in vitro |b Modulation by luminal Ammonia and pH |c vorgelegt von Zhongyan Lu |
| 246 | 1 | 3 | |a Untersuchungen über den Transport von Harnstoff des ovinen Pansenepithels |
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Datensatz im Suchindex
| _version_ | 1804151738331037696 |
|---|---|
| adam_text | CONTENTS
CONTENTS
GENERAL INTRODUCTION 1
1.1 INTRODUCTION 2
1.2 REVIEW OF LITERATURE 5
1.2.1 RUMINANTS 5
1.2.2 RUMEN 6
1.2.3 RUMEN ENVIRONMENT 7
1.2.4 RUMEN FERMENTATION: CARBOHYDRATES 10
1.2.5 RUMEN NITROGEN METABOLISM 12
1.2.6 UREA AND UREA RECYCLING IN RUMINANTS 15
1.2.7 SCFA PRODUCTION AND ABSORPTION IN THE RUMEN 20
1.2.8 SCFA, C0
2
, AND RUMINAL UREA TRANSPORT 21
1.2.9 AMMONIA*... 23
1.2.10 RUMINAL AMMONIA TRANSPORT AND POTASSIUM CHANNEL 25
1.2.11 RUMINAL AMMONIA ABSORPTION AND MODULATION BY ELECTRICAL
GRADIENTS 28
1.2.12 MODULATION OF AMMONIA TRANSPORT BY CO2 AND SCFA 30
1.2.13 UREA TRANSPORTER.... 32
1.2.14 REGULATION OF UT ACTIVITIES 37
MATERIAL AND METHODS 39
2.1 EXPERIMENTAL ANIMALS AND FEEDING 40
2.2 RUMEN EPITHELIUM ISOLATION AND INCUBATION 40
2.3 USSING CHAMBER TECHNIQUE 41
2.3.1 ELECTRICAL MEASUREMENTS 42
2.3.2 OPEN CIRCUIT CONDITIONS 43
2.3.4 SHORT-CIRCUIT CONDITIONS.. 43
2.3.5 CALCULATION OF ION FLUX RATES 44
2.3.6 MEASUREMENT OF FLUXES 45
2.3.7 MODIFICATIONS OF USSING CHAMBER TECHNIQUE 46
. 2.4 INHIBITORS 47
2.5 FULL SEQUENCE OF UT-B 49
2.6 STATISTICS 50
RESULTS 54
3.1 UREA FLUX MEASUREMENTS IN USSING CHAMBER 55
3.1.1 EFFECT OF AMMONIA ON UREA FLUX AT PH 7.4 AND 6.4 55
3.1.2 EFFECT OF METHYLAMMONIUM CHLORIDE 59
3.1.3 EFFECT OF TRANSEPITHELIAL POTENTIAL DIFFERENCE, PD
T
, ON UREA
TRANSPORT RATES 60
3.1.4 EFFECT OF LUMINAL K CONCENTRATION ON UREA TRANSPORT 61
3.1.5 MODULATION OF UREA TRANSPORT BY SHORT CHAIN FATTY ACIDS (SCFA)62
3.1.6 UREA TRANSPORT AND TISSUE CONDUCTANCE 64
3.1.7 MODULATION OF UREA TRANSPORT BY LUMINAL PH 66
3.1.8 EFFECT OF CHANNEL BLOCKERS 70
HTTP://D-NB.INFO/1048020908
CONTENTS
3.2 SEQUENCING OF UREA TRANSPORT B IN SHEEP 72
3.3 INTERACTION BETWEEN AMMONIA AND SCFA 74
3.4 MODULATION OF NA TRANSPORT 75
DISCUSSION AND CONCLUSION 79
4.1 EFFECT OF AMMONIA ON UREA TRANSPORT: NH
3
OR NH
4+
.. 81
4.2 DOSE-RESPONSE CURVE OF NH
4+
85
4.3 SHORT CHAIN FATTY ACIDS AND CARBON DIOXIDE 86
4.4 EFFECT OF LUMINAL PH 88
4.5 THE PATHWAY OF UREA ACROSS THE RUMEN EPITHELIUM 89
4.6 CDNA SEQUENCE OF RUMINAL UT-B IN SHEEP 89
4.7 UPTAKE OF NH
4+
AND INTRACELLULAR PH 90
4.8 EFFECTS OF PROTONS AND AMMONIA ON UREA TRANSPORT: ACID SENSING OR
CHANNEL GATING? 91
4.9 UREA RECYCLING AND POLLUTION OF THE ENVIRONMENT 94
4.10 CONCLUSION 95
SUMMARY 97
REFERENCES 103
ACKNOWLEDGMENTS 113
|
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| author | Lu, Zhongyan 1980- |
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| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
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| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| spelling | Lu, Zhongyan 1980- Verfasser (DE-588)104629766X aut Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH vorgelegt von Zhongyan Lu Untersuchungen über den Transport von Harnstoff des ovinen Pansenepithels 2013 114 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Untersuchungen über den Transport von Harnstoff des ovinen Pansenepithels Berlin, Freie Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000095878-5 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000095878 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027001283&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lu, Zhongyan 1980- Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH |
| subject_GND | (DE-588)4113937-9 |
| title | Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH |
| title_alt | Untersuchungen über den Transport von Harnstoff des ovinen Pansenepithels |
| title_auth | Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH |
| title_exact_search | Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH |
| title_full | Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH vorgelegt von Zhongyan Lu |
| title_fullStr | Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH vorgelegt von Zhongyan Lu |
| title_full_unstemmed | Urea transport in sheep rumen epithelium in vitro Modulation by luminal Ammonia and pH vorgelegt von Zhongyan Lu |
| title_short | Urea transport in sheep rumen epithelium in vitro |
| title_sort | urea transport in sheep rumen epithelium in vitro modulation by luminal ammonia and ph |
| title_sub | Modulation by luminal Ammonia and pH |
| topic_facet | Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000095878 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=027001283&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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