Tracking chaperone-mediated folding using force spectroscopy:
Gespeichert in:
1. Verfasser: | |
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Format: | Abschlussarbeit Buch |
Sprache: | English |
Veröffentlicht: |
2013
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Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XXII, 149 S. Ill., graph. Darst. |
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Datensatz im Suchindex
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adam_text | CONTENTS
LIST OF ABBREVIATIONS XI
LIST OF SYMBOLS XIII
LIST OF FIGURES XVII
LIST OF TABLES XXI
1 GENERAL INTRODUCTION 1
1.1 PROTEIN FOLDING 2
1.1.1 FOLDING MECHANISMS 3
1.1.2 THE EFFECTS OF MACROMOLECULAR CROWDING 5
1.1.3 CONSEQUENCES OF MISFOLDING AND AGGREGATION 6
1.2 MOLECULAR CHAPERONES 7
1.2.1 THE HSP70 SYSTEM 8
1.2.2 THE CHAPERONE DNAK 8
1.2.3 THE CO-CHAPERONE DNAJ 9
1.2.4 THE CO-CHAPERONE GRPE 11
1.2.5 THE HSP70 CHAPERONE CYCLE 11
1.2.6 TECHNIQUES USED TO STUDY PROTEIN FOLDING 14
1.3 THE ATOMIC FORCE MICROSCOPE 14
1.3.1 CANTILEVERS 16
1.3.2 SPRING CONSTANT CALIBRATION 17
1.3.3 USES OF AFM 18
HTTP://D-NB.INFO/1046140892
VIII
1.4 FORCE SPECTROSCOPY 20
1.4.1 CONSTANT FORCE PULLING 22
1.4.2 CONSTANT SPEED PULLING 23
1.4.3 DYNAMIC FORCE SPECTROSCOPY 25
1.4.4 FORCE-DISTANCE CURVES 26
1.5 POLYMER EXTENSION MODELS 28
1.5.1 THE GAUSSIAN CHAIN 28
1.5.2 THE FREELY JOINTED CHAIN 29
1.5.3 THE WORMLIKE CHAIN 30
1.6 IMMUNOGLOBULIN 27 OF THE HUMAN MUSCLE PROTEIN TITIN 30
1.7 BIBLIOGRAPHY 33
2 DESIGNING TOOLS FOR AUTOMATED DATA ACQUISITION WITH THE
ATOMIC FORCE MICROSCOPE 45
2.1 EXPERIMENTAL SECTION 48
2.1.1 MAKING SUPPORTS FOR SMFS 48
2.1.2 MAKING OF THE O-RING 50
2.1.3 FUNCTIONALIZING GOLD-COATED BL-RC150VB WITH NI-NTA
GROUPS 50
2.1.4 EXPRESSING PROTEINS FOR SMFS 52
2.1.5 FORCE SPECTROSCOPY EXPERIMENTS 54
2.1.6 ANALYSIS 56
2.2 RESULTS 58
2.3 DISCUSSION 62
2.4 CONCLUSIONS 63
2.5 BIBLIOGRAPHY 63
3 DYNAMIC FORCE SPECTROSCOPY OF 127 DOMAINS 65
3.1 EXPERIMENTAL SECTION 67
3.1.1 EXPERIMENTAL SETUP 67
3.1.2 DYNAMIC FORCE SPECTROSCOPY EXPERIMENTS 67
IX
3.1.3 DATA ANALYSIS 68
3.2 RESULTS 70
3.3 DISCUSSION 75
3.4 CONCLUSIONS 76
3.5 BIBLIOGRAPHY 77
4 DIRECT ASSAY OF PROTEIN UNFOLDING KINETICS USING FORCE-
HISTORY ANALYSIS 81
4.1 EXPERIMENTAL SECTION 84
4.2 RESULTS 87
4.3 DISCUSSION 95
4.4 CONCLUSIONS 96
4.5 BIBLIOGRAPHY 97
5 REFOLDING AND MISFOLDING OF ENGINEERED HOMOMERIC REPEATS
OF TITIN S 127 DOMAIN 101
5.1 EXPERIMENTAL SECTION 102
5.1.1 EXPERIMENTAL SETUP AND REFOLDING EXPERIMENTS 102
5.1.2 DATA ANALYSIS 102
5.2 RESULTS 107
5.3 DISCUSSION 110
5.4 CONCLUSIONS 111
5.5 BIBLIOGRAPHY 112
6 STUDYING THE EFFECT OF THE HSP70 CHAPERONE SYSTEM ON
PROTEIN FOLDING USING SINGLE-MOLECULE FORCE SPECTROSCOPY 115
6.1 EXPERIMENTAL SECTION 117
6.1.1 UNFOLDING OF MBP 117
6.1.2 REFOLDING EXPERIMENTS 117
6.2 RESULTS 118
6.3 DISCUSSION 134
6.4 CONCLUSIONS 139
X
6.5 BIBLIOGRAPHY 140
7 OUTLOOK 145
7.1 BIBLIOGRAPHY 146
ACKNOWLEDGEMENTS 147
ERKLARUNG 149
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any_adam_object | 1 |
author | Nunes, João Manuel das Eiras 1982- |
author_GND | (DE-588)1045331503 |
author_facet | Nunes, João Manuel das Eiras 1982- |
author_role | aut |
author_sort | Nunes, João Manuel das Eiras 1982- |
author_variant | j m d e n jmde jmden |
building | Verbundindex |
bvnumber | BV041488165 |
classification_rvk | WD 5100 |
ctrlnum | (OCoLC)878159274 (DE-599)BSZ398952884 |
dewey-full | 572.633 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572.633 |
dewey-search | 572.633 |
dewey-sort | 3572.633 |
dewey-tens | 570 - Biology |
discipline | Biologie |
format | Thesis Book |
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indexdate | 2024-07-10T00:57:57Z |
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spelling | Nunes, João Manuel das Eiras 1982- Verfasser (DE-588)1045331503 aut Tracking chaperone-mediated folding using force spectroscopy von João Manuel das Eiras Nunes 2013 XXII, 149 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Dresden, Techn. Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026934022&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Nunes, João Manuel das Eiras 1982- Tracking chaperone-mediated folding using force spectroscopy |
subject_GND | (DE-588)4113937-9 |
title | Tracking chaperone-mediated folding using force spectroscopy |
title_auth | Tracking chaperone-mediated folding using force spectroscopy |
title_exact_search | Tracking chaperone-mediated folding using force spectroscopy |
title_full | Tracking chaperone-mediated folding using force spectroscopy von João Manuel das Eiras Nunes |
title_fullStr | Tracking chaperone-mediated folding using force spectroscopy von João Manuel das Eiras Nunes |
title_full_unstemmed | Tracking chaperone-mediated folding using force spectroscopy von João Manuel das Eiras Nunes |
title_short | Tracking chaperone-mediated folding using force spectroscopy |
title_sort | tracking chaperone mediated folding using force spectroscopy |
topic_facet | Hochschulschrift |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026934022&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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