Gigaseal Formation in Patch Clamping: With Applications of Nanotechnology
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
2014
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| Schriftenreihe: | SpringerBriefs in Applied Sciences and Technology
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| Schlagworte: | |
| Online-Zugang: | BTU01 FHA01 FHI01 FHN01 FHR01 FKE01 FRO01 FWS01 FWS02 UBY01 Volltext Inhaltsverzeichnis Abstract |
| Beschreibung: | This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation |
| Beschreibung: | 1 Online-Ressource (XI, 94 p.) 81 illus., 65 illus. in color |
| ISBN: | 9783642391286 |
| DOI: | 10.1007/978-3-642-39128-6 |
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| 505 | 0 | |a Chapter 1 Introduction -- Chapter 2 Development of patch clamping -- Chapter 3 Gigaseal formation -- Chapter 4 Effect of roughness on gigaseal formation -- Chapter 5 Effect of hydrophilicIty on gigaseal formation -- Chapter 6 Effect of tip size on gigaseal formation -- Chapter 7 Study of glass micropipettes from tip formation to characterization -- References | |
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Datensatz im Suchindex
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| adam_text | GIGASEAL FORMATION IN PATCH CLAMPING
/ MALBOUBI, MAJID
: 2014
TABLE OF CONTENTS / INHALTSVERZEICHNIS
CHAPTER 1 INTRODUCTION
CHAPTER 2 DEVELOPMENT OF PATCH CLAMPING
CHAPTER 3 GIGASEAL FORMATION
CHAPTER 4 EFFECT OF ROUGHNESS ON GIGASEAL FORMATION
CHAPTER 5 EFFECT OF HYDROPHILICITY ON GIGASEAL FORMATION
CHAPTER 6 EFFECT OF TIP SIZE ON GIGASEAL FORMATION
CHAPTER 7 STUDY OF GLASS MICROPIPETTES FROM TIP FORMATION TO
CHARACTERIZATION
REFERENCES
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
GIGASEAL FORMATION IN PATCH CLAMPING
/ MALBOUBI, MAJID
: 2014
ABSTRACT / INHALTSTEXT
THIS BOOK PRESENTS AN INVESTIGATION OF GIGASEAL FORMATION USING
MICRO/NANOTECHNOLOGY. THE AIMS OF THE BOOK ARE TWOFOLD. FIRST, IT
EXPLAINS THE MECHANISMS OF GIGASEAL FORMATION USING THE LATEST
DISCOVERIES. SECOND, IT PROVIDES PRACTICAL TECHNIQUES FOR FREQUENT
FORMATION OF HIGH RESISTANCE SEALS. THE FORMATION OF A HIGH-RESISTANCE
ELECTRICAL SEAL, ALSO KNOWN AS A GIGASEAL, BETWEEN A CELL MEMBRANE AND A
GLASS MICROPIPETTE TIP IS ESSENTIAL IN PATCH-CLAMP EXPERIMENTS. EVEN
THOUGH FOUR DECADES HAVE PASSED SINCE THE INTRODUCTION OF THE
PATCH-CLAMPING TECHNIQUE BY NEHER AND SAKMANN, GIGASEAL FORMATION
REMAINS AN OBSTACLE IN DEVELOPING THE HIGH-THROUGHPUT ION CHANNEL
SCREENING SYSTEMS REQUIRED BY THE PHARMACEUTICAL INDUSTRY. HERE THE
AUTHORS SHARE THEIR LATEST METHODS FOR ACHIEVING GIGASEAL FORMATION AND
DESCRIBE TECHNIQUES THAT ARE HIGHLY DESIRABLE AT BOTH RESEARCH AND
INDUSTRIAL LEVELS. NANOTECHNOLOGY HAS BEEN FOUND TO BE A POWERFUL TOOL
FOR STUDYING AND MODIFYING GLASS MICROPIPETTES AND IN TACKLING THE
PROBLEM OF GIGASEAL FORMATION
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
|
| any_adam_object | 1 |
| author | Malboubi, Majid |
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| bvnumber | BV041471037 |
| collection | ZDB-2-ENG |
| contents | Chapter 1 Introduction -- Chapter 2 Development of patch clamping -- Chapter 3 Gigaseal formation -- Chapter 4 Effect of roughness on gigaseal formation -- Chapter 5 Effect of hydrophilicIty on gigaseal formation -- Chapter 6 Effect of tip size on gigaseal formation -- Chapter 7 Study of glass micropipettes from tip formation to characterization -- References |
| ctrlnum | (OCoLC)867568441 (DE-599)BVBBV041471037 |
| dewey-full | 620.5 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 620 - Engineering and allied operations |
| dewey-raw | 620.5 |
| dewey-search | 620.5 |
| dewey-sort | 3620.5 |
| dewey-tens | 620 - Engineering and allied operations |
| doi_str_mv | 10.1007/978-3-642-39128-6 |
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| illustrated | Illustrated |
| indexdate | 2024-08-01T10:55:48Z |
| institution | BVB |
| isbn | 9783642391286 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026917179 |
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| physical | 1 Online-Ressource (XI, 94 p.) 81 illus., 65 illus. in color |
| psigel | ZDB-2-ENG |
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| record_format | marc |
| series2 | SpringerBriefs in Applied Sciences and Technology |
| spellingShingle | Malboubi, Majid Gigaseal Formation in Patch Clamping With Applications of Nanotechnology Chapter 1 Introduction -- Chapter 2 Development of patch clamping -- Chapter 3 Gigaseal formation -- Chapter 4 Effect of roughness on gigaseal formation -- Chapter 5 Effect of hydrophilicIty on gigaseal formation -- Chapter 6 Effect of tip size on gigaseal formation -- Chapter 7 Study of glass micropipettes from tip formation to characterization -- References Engineering Biomedical engineering Nanotechnology and Microengineering Biomedical Engineering Ingenieurwissenschaften Patch-Clamp-Methode (DE-588)4279564-3 gnd |
| subject_GND | (DE-588)4279564-3 |
| title | Gigaseal Formation in Patch Clamping With Applications of Nanotechnology |
| title_auth | Gigaseal Formation in Patch Clamping With Applications of Nanotechnology |
| title_exact_search | Gigaseal Formation in Patch Clamping With Applications of Nanotechnology |
| title_full | Gigaseal Formation in Patch Clamping With Applications of Nanotechnology by Majid Malboubi, Kyle Jiang |
| title_fullStr | Gigaseal Formation in Patch Clamping With Applications of Nanotechnology by Majid Malboubi, Kyle Jiang |
| title_full_unstemmed | Gigaseal Formation in Patch Clamping With Applications of Nanotechnology by Majid Malboubi, Kyle Jiang |
| title_short | Gigaseal Formation in Patch Clamping |
| title_sort | gigaseal formation in patch clamping with applications of nanotechnology |
| title_sub | With Applications of Nanotechnology |
| topic | Engineering Biomedical engineering Nanotechnology and Microengineering Biomedical Engineering Ingenieurwissenschaften Patch-Clamp-Methode (DE-588)4279564-3 gnd |
| topic_facet | Engineering Biomedical engineering Nanotechnology and Microengineering Biomedical Engineering Ingenieurwissenschaften Patch-Clamp-Methode |
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