The role of inflammation during zebrafish central nervous system regeneration:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 75 Bl. Ill., graph. Darst. |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
ACKNOWLEDGEMENTS 4
LIST OF PUBLICATIONS 6
1 SUMMARY 7
2 INTRODUCTION 10
2.1 TISSUE AND ORGAN REGENERATION: DEFINITION AND HISTORIC MILESTONES 10
2.2 TISSUE REGENERATION IN NON-MAMMALIAN VERTEBRATES 12
2.3 REGENERATION OF THE CENTRAL NERVOUS SYSTEM 12
2.3.1 CELL DEATH 16
2.3.2 REACTIVE PROLIFERATION 16
2.3.3 REGENERATIVE NEUROGENESIS 17
2.4 NEUROINFLAMMATION 18
2.4.1 MICROGLIA 18
2.4.2 LEUKOCYTE INVASION 19
2.4.3 MOLECULAR CUES 20
2.5 AIMS OF THIS STUDY 23
3 MATERIALS AND METHODS 24
3.1 MATERIALS 24
3.1.1 TECHNICAL EQUIPMENT 24
3.1.2 TRANSGENIC LINES 24
3.1.3 KITS 24
3.1.4 REAGENTS AND BUFFERS 25
3.1.5 PRIMARY ANTIBODIES 26
3.1.6 S ECONDARY ANTIBODIES 2 6
3.1.7 PCR PRIMERS 2 6
3.2 METHODS 27
3.2.1 FISH MAINTENANCE 27
3.2.2 STAB LESION ASSAY 27
3.2.3 BRDU LABELING 27
3.2.4 PREPARATION OF CRYO-SECTIONS 28
3.2.5 PREPARATION OF PARAFFIN SECTIONS 28
3.2.6 HAEMATOXYLIN AND EOSIN DOUBLE-STAIN 28
3.2.7 IMMUNOHISTOCHEMISTRY 28
3.2.7.1 BRDU STAINING OF SECTIONS 28
3.2.7.2 PCNA STAINING OF SECTIONS 28
HTTP://D-NB.INFO/1046653822
3.2.7.3 HUC/D STAINING OF SECTIONS 28
3.2.8
IN SITU
HYBRIDISATION AND PROBE SYNTHESIS 29
3.2.8.1 GENERATION OF NEW
IN SITU
PROBES 29
3.2.8.2 PLASMID LINEARIZATION 30
3.2.8.3 TRANSCRIPTION AND PURIFICATION OF THE
IN SITU
PROBE 30
3.2.8.4
IN SITU
HYBRIDIZATION ON CRYO-SECTIONS 30
3.2.9 TUNEL CELL DEATH ASSAY 31
3.2.10 IMMUNOSUPPRESSION ASSAY 31
3.2.11 CEREBROVENTRICULAR MICROINJECTION (CVMI) 31
3.2.12 PRANLUKAST TREATMENTS 32
3.2.13 IMAGE ACQUISITION AND PROCESSING 32
3.2.14 QUANTIFICATION AND STATISTICAL ANALYSES 32
4 RESULTS 33
4.1 TRANSCRIPTOME ANALYSIS SUGGESTS A ROLE OF INFLAMMATION DURING
CNS REGENERATION 33
4.2 CHARACTERIZATION OF THE IMMUNE RESPONSE UPON TBI 34
4.3 MIMICKING AN INFLAMMATORY RESPONSE WITHOUT TBI 35
4.4 INDUCED INFLAMMATION IS SUFFICIENT TO INCREASE THE PROLIFERATION
OF RADIAL GLIA AND THE PRODUCTION ON NEW NEURONS 38
4.5 INFLAMMATION IS NECESSARY FOR THE INITIATION OF THE REGENERATIVE
RESPONSE AFTER TBI 39
4.6 INFLAMMATION IS NECESSARY FOR CAUDAL FIN REGENERATION 42
4.7 CYSTEINYL LEUKOTRIENE SIGNALING IS NECESSARY FOR THE INITIATION
OF REACTIVE PROLIFERATION AND REGENERATIVE NEUROGENESIS 42
4.8 CYSLTL - LTC4 IS SUFFICIENT TO INDUCE REACTIVE PROLIFERATION
AND NEUROGENESIS 44
4.9 INDUCED INFLAMMATION INITIATES REGENERATION SPECIFIC MOLECULAR
PROGRAMS IN THE ADULT ZEBRAFISH BRAIN 47
5 DISCUSSION 50
5.1 THE SOURCE OF THE NEURAL PROGENITOR CELL ACTIVATION 50
5.2 TEMPORAL RESOLUTION OF THE INFLAMMATORY RESPONSE 50
5.3 REACTIVE NEUROGENESIS AFTER TBI VS INDUCED INFLAMMATION 52
5.4 IMMUNOSUPPRESSION ASSAYS 53
5.5 ACTIVATION OF THE REGENERATIVE RESPONSE BY SECRETED CUES
54
2
5.6 INFLAMMATION AND REGENERATION IN NON-MAMMALIAN VERTEBRATES 56
5.7 CONCLUSIONS AND OUTLOOK 59
6 APPENDIX 60
7 REFERENCES 62
8 ABBREVIATIONS 73
9 DECLARATION ACCORDING TO §5.5 OF THE DOCTORATE REGULATIONS 75
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| spelling | Kyritsis, Nikolaos 1985- Verfasser (DE-588)1043248889 aut The role of inflammation during zebrafish central nervous system regeneration von Nikolaos Kyritsis 2013 75 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Dresden, Techn. Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026909753&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kyritsis, Nikolaos 1985- The role of inflammation during zebrafish central nervous system regeneration |
| subject_GND | (DE-588)4113937-9 |
| title | The role of inflammation during zebrafish central nervous system regeneration |
| title_auth | The role of inflammation during zebrafish central nervous system regeneration |
| title_exact_search | The role of inflammation during zebrafish central nervous system regeneration |
| title_full | The role of inflammation during zebrafish central nervous system regeneration von Nikolaos Kyritsis |
| title_fullStr | The role of inflammation during zebrafish central nervous system regeneration von Nikolaos Kyritsis |
| title_full_unstemmed | The role of inflammation during zebrafish central nervous system regeneration von Nikolaos Kyritsis |
| title_short | The role of inflammation during zebrafish central nervous system regeneration |
| title_sort | the role of inflammation during zebrafish central nervous system regeneration |
| topic_facet | Hochschulschrift |
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