Receptor-receptor interactions:
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2013
|
Ausgabe: | 1. ed. |
Schriftenreihe: | Methods in cell biology
117 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XXI, 493 S., [9] Bl. Ill., graph. Darst. |
ISBN: | 9780124081437 |
Internformat
MARC
LEADER | 00000nam a2200000 cb4500 | ||
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250 | |a 1. ed. | ||
264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2013 | |
300 | |a XXI, 493 S., [9] Bl. |b Ill., graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
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Datensatz im Suchindex
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---|---|
adam_text | Contents
Contributors
...........................................................................................................xiii
Preface
....................................................................................................................xxi
CHAPTER
1
Spatial Intensity Distribution Analysis (SplDA):
A New Tool for Receptor Tyrosine Kinase
Activation and Transactivation Quantification
.............
ι
Annie Barbeau,
Jody
L.
Swift,
Antoine G.
Godin,
Yves De Koninck, Paul W.
Wiseman,
Jean-Martin Beaulieu
Introduction
..................................................................................2
1.1
Theory of
Spatial
Intensity
Distribution
Analysis.......................
4
1.2
SplDA:
Examples of
Application
to RTK
..................................4
1.3
Procedure for
SplDA
...................................................................6
1.4
Discussion
..................................................................................17
Acknowledgments
.....................................................................18
References..................................................................................
18
CHAPTER
2
Dimerization
of Nuclear Receptors
................................21
Pierre Germain, William Bourguet
Introduction
................................................................................22
2.1
Methods
......................................................................................24
Acknowledgments
.....................................................................39
References
..................................................................................39
CHAPTER
3
Network Analysis to Uncover the Structural
Communication in GPCRs
..................................................43
Francesca
Fanelli, Angelo
Felline,
Francesco Raimondi
Introduction
................................................................................44
3.1
Materials
.....................................................................................45
3.2
Methods
......................................................................................45
3.3
Discussion
..................................................................................50
Summary
....................................................................................56
Acknowledgments
.....................................................................59
References
..................................................................................59
CHAPTER
4
Simulating
G
Protein-Coupled Receptors
in Native-Like Membranes: From Monomers
to
Oligomers
...........................................................................63
Ramon
Guixà-González,
Juan Manuel Ramirez-Anguita,
Agnieszka A. Kaczor,
Jana
Seleni
Introduction
................................................................................64
4.1
Membranes
.................................................................................65
4.2
GPCR Monomers in Membranes
...............................................72
4.3
GPCR Dimers and Oligomers in Membranes
...........................80
References
..................................................................................83
CHAPTER
5
Structure-Based Molecular Modeling
Approaches to GPCR Oligomerization
...........................91
Agnieszka A. Kaczor,
Jana
Seleni,
Antti
Poso
Introduction
................................................................................92
5.1
Protein-Protein Docking
............................................................93
5.2 MD
Simulation
...........................................................................96
5.3
Normal Mode Analysis
..............................................................99
5.4
Electrostatics Studies
...............................................................100
Acknowledgments
...................................................................101
References
................................................................................102
CHAPTER
б
Biochemical and Imaging Methods to Study
Receptor Membrane Organization and
Association with
Lipid
Rafts
...........................................
ios
Bruno M. Castro,
Juan A. Torreno-Piña,
Thomas S. van
Zanten,
Maria F. Gracia-Parajo
Introduction and Rationale
.......................................................106
6.1
Methods to Determine Protein Association with
Lipid
Rafts
................................................................................107
6.2
Optical Techniques to Study Receptor Membrane
Organization and Association with
Lipid
Rafts
......................110
Concluding Remarks
...............................................................118
Acknowledgments
...................................................................119
References
................................................................................119
CHAPTER
7
Serotonin Type
4
Receptor Dimers
..............................
ш
Sylvie
Claeysen,
Romain
Donneger,
Patrizia Giannoni,
Florence Gaven,
Lucie
P. Pellissier
Introduction
..............................................................................124
7.1
Materials
...................................................................................125
7.2
Methods
....................................................................................127
7.3
Discussion
................................................................................136
Summary
..................................................................................137
Acknowledgments
...................................................................137
References
................................................................................138
CHAPTER
8
Bioluminescence Resonance Energy Transfer
Methods to Study
G
Protein-Coupled
Receptor-Receptor Tyrosine Kinase
Heteroreceptor
Complexes
..............................................
i4i
Dasiel
0.
Borroto-Escuela, Marc Flajolet,
Luigi
F.
Agnati,
Paul Greengard,
Kjell
Fuxe
Introduction
..............................................................................142
8.1
The Method Principle
..............................................................143
8.2
Setting Up a BRET Assay
.......................................................146
8.3
Methods and Detailed Protocols
..............................................148
8.4
Discussion and Note on Critical Parameters
...........................159
Acknowledgments
...................................................................161
References
................................................................................162
CHAPTER
9
A Simple Method to Detect Allostery
in GPCR Dimers
...................................................................165
Eugénie Goupil, Stéphane
A. Laporte, Terence
E.
Hébert
Introduction
and Rationale
.......................................................166
9.1
Materials
...................................................................................169
9.2
Methods
....................................................................................170
9.3
Discussion
................................................................................174
Summary
..................................................................................176
Acknowledgments...................................................................
176
References
................................................................................176
CHAPTER
10
Fluorescence Correlation Spectroscopy
and Photon-Counting Histogram Analysis
of Receptor-Receptor Interactions
..............................
m
Katharine Herrick-Davis, Joseph E. Mazurkiewicz
Introduction
..............................................................................182
10.1
Materials
...................................................................................184
10.2
Methods
....................................................................................185
10.3
Discussion
................................................................................194
Summary
..................................................................................195
Acknowledgments
...................................................................195
References
................................................................................195
CHAPTER
11
Monitoring Receptor Oligomerization
by Line-Scan Fluorescence Cross-Correlation
SpeCtrOSCOpy
.......................................................................197
Mark
A. Hink,
Marten Postma
Introduction
..............................................................................198
11.1
Materials
...................................................................................199
11.2
Methods
....................................................................................202
11.3
Discussion
................................................................................209
Acknowledgments
...................................................................210
References
................................................................................210
CHAPTER
12
Biochemical Assay of
G
Protein-Coupled Receptor
Oligomerization: Adenosine ^ and Thromboxane
A2 Receptors Form the Novel Functional
Hetero-oligomer
..................................................................213
Natsumi Mizuno, Tokiko Suzuki, Yu Kishimoto,
Noriyasu Hirasawa
Introduction
..............................................................................214
12.1
Detection of GPCR Oligomerization
.......................................216
12.2
Measurement of the Signaling Pathway
..................................220
12.3
Materials and Methods
.............................................................223
Conclusion
................................................................................224
References
................................................................................224
CHAPTER
13
Oligomerization of Sweet and Bitter Taste
Receptors
..............................................................................229
Christina
Kuhn,
Wolfgang Meyerhof
Introduction and Rationale
.......................................................230
13.1
Materials
...................................................................................232
13.2
Methods
....................................................................................233
13.3
Discussion
................................................................................240
Summary
..................................................................................241
Acknowledgments
...................................................................241
References
................................................................................241
CHAPTER
14
Analysis of Receptor-Receptor Interaction
by Combined Application of FRET and
MicrOSCOpy
...........................................................................243
Sonai
Prasad,
Andre
Zeug,
Evgeni Ponimaskin
Introduction and Rationale
.......................................................244
14.1
Theory
......................................................................................245
14.2
Materials
/
Experimental Setups
..............................................249
14.3
Methods (Protocols and Procedure)
.........................................253
14.4
Results and Discussion
............................................................263
Acknowledgments
...................................................................264
References
................................................................................264
CHAPTER
15
Site-Specific Labeling of Genetically Encoded
Azido Groups for Multicolor, Single-Molecule
Fluorescence Imaging of GPCRs
...................................267
He
Tian,
Thomas P. Sakmar, Thomas Huber
15.1
Purpose
.....................................................................................270
15.2
Theory
......................................................................................270
15.3
Protocol
1 :
Genetically Encoded Azido Groups for
Bioorthogonal
Conjugation
......................................................273
15.4
Protocol
2:
Labeling with Fluorescent Probes Using
Cyclooctynes
............................................................................276
15.5
Protocol
3:
Preparation of Biotinylated Antibodies
................282
15.6
Protocol
4:
Preparation of Detergent-Solubilized Lipids
........285
15.7
Protocol
5:
Single-Molecule Immunoprecipitation on
Glass-Bottom
Microplates
.......................................................287
15.8
Protocol
6:
Automated Multicolor, Single-Molecule
TIRF Microscopy
.....................................................................293
Acknowledgments
...................................................................302
References
................................................................................302
CHAPTER
16
Analysis of EGF Receptor Oligomerization
by Homo-FRET
......................................................................
305
Cecilia
de Heus,
Nivard Kagie, Raimond Heukers,
Paul M.P. van Bergen en
Henegouwen,
Hans C.
Gerritsen
Introduction
..............................................................................306
16.1
Theory Homo-FRET Quantification
........................................309
16.2
Materials
...................................................................................313
16.3
Methods
....................................................................................316
16.4
Discussion
................................................................................317
Acknowledgments
...................................................................319
References
................................................................................320
CHAPTER
17
Detection of
G
Protein-Coupled
Receptor (GPCR) Dimerization by
Coimmunoprecipitation
....................................................
323
Kamila Skieterska,
Jolién
Duchou,
Beatrice
Lintermans,
Kathleen Van Craenenbroeck
Introduction
..............................................................................324
17.1
Materials
...................................................................................326
17.2
Methods
....................................................................................327
17.3
Discussion
................................................................................338
Acknowledgments
...................................................................339
References
................................................................................339
CHAPTER
18
Lipid-Dependent GPCR Dimerization
............................341
Alan
D.
Goddard, Patricia
M.
Dijkman,
Roslin J. Adamson,
Anthony Watts
Introduction..............................................................................
342
18.1
Materials
and Method
..............................................................343
18.2
FRET Measurements and Analysis
.........................................351
18.3
Interpreting Results
..................................................................354
Summary
..................................................................................355
References
................................................................................355
CHAPTER
19
Monitoring Peripheral Protein Oligomerization
on Biological Membranes
...............................................359
Robert V. Stahelin
Introduction
..............................................................................360
19.1
Materials and Methods
.............................................................362
19.2
Considerations
..........................................................................365
Summary and Conclusion
........................................................366
Acknowledgments
...................................................................369
References
................................................................................369
CHAPTER
20
Single-Molecule Imaging of Receptor-Receptor
Interactions
...........................................................................373
Kenichi G.N. Suzuki, Rinshi S.
Kasai,
Takahiro K. Fujiwara,
Akihiro Kusumi
Introduction
..............................................................................374
20.1
Receptor Expression and Fluorescent Labeling
......................376
20.2
Single-Molecule Imaging
.........................................................380
20.3
Data Analysis
...........................................................................382
Acknowledgments
...................................................................387
References
................................................................................387
CHAPTER
21
Visualization of TCR Nanoclusters via
Immunogold Labeling, Freeze-Etching,
and Surface Replication
..................................................391
Gina
J.
Fiala,
María Teresa Rejas,
Wolfgang W.
Schamel,
Hisse M.
van
Santen
Introduction
..............................................................................393
21.1
Materials
...................................................................................394
21.2
Equipment
................................................................................395
21.3
Methods
....................................................................................395
21.4
Analysis
....................................................................................405
21.5
Considerations
..........................................................................405
Acknowledgments
...................................................................408
References
................................................................................408
CHAPTER
22
Identification of Multimolecular Complexes
and
Supercomplexes
in Compartment-Selective
Membrane Microdomains
................................................411
Panagiotis Mitsopoulos,
Joaquín Madreñas
Introduction and Rationale
.......................................................413
22.1
Detergent-Insoluble Glycosphingolipid (DIG)
Microdomain
Isolation
.............................................................415
22.2
Plasma Membrane Isolation
.....................................................418
22.3
Cardiolipin-Enriched Mitochondrial Membrane
Microdomain
Isolation
.............................................................421
22.4
Mitochondrial Supercomplex Identification
by Blue-Native Gel Electrophoresis
........................................423
22.5
Discussion
................................................................................428
Summary
..................................................................................429
Acknowledgments
...................................................................429
References
................................................................................430
CHAPTER
23
G
Protein-Coupled Receptor Transactivation:
From Molecules to Mice
..................................................433
Kim
С
Jonas,
Adolfo
Rivero-Müller,
lipo
T.
Huhtaniemi,
Aylin
С.
Hanyaloglu
Introduction
..............................................................................434
23.1
Materials
...................................................................................435
23.2
Methods
....................................................................................436
23.3
Discussion
................................................................................447
Summary
..................................................................................448
Acknowledgments
...................................................................448
References
................................................................................448
CHAPTER
24
Crystallization of
G
Protein-Coupled Receptors
......451
David
Salom,
Pius S. Padayatti,
Krzysztof Palczewski
Introduction
..............................................................................452
24.1
Crystallization of Bovine Rhodopsin
......................................453
24.2
Crystallization of
ß2-AR.........................................................460
24.3
Discussion
................................................................................465
Acknowledgments
...................................................................465
References
................................................................................466
Index
.......................................................................................................................469
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genre_facet | Aufsatzsammlung |
id | DE-604.BV041457348 |
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indexdate | 2024-07-10T00:57:09Z |
institution | BVB |
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physical | XXI, 493 S., [9] Bl. Ill., graph. Darst. |
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spelling | Receptor-receptor interactions ed. by P. Michael Conn 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2013 XXI, 493 S., [9] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 117 Rezeptor (DE-588)4049722-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Rezeptor (DE-588)4049722-7 s Wechselwirkung (DE-588)4064937-4 s Methode (DE-588)4038971-6 s b DE-604 Conn, Patrick Michael 1949-2016 Sonstige (DE-588)136758541 oth Methods in cell biology 117 (DE-604)BV002534878 117 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026903774&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Receptor-receptor interactions Methods in cell biology Rezeptor (DE-588)4049722-7 gnd Methode (DE-588)4038971-6 gnd Wechselwirkung (DE-588)4064937-4 gnd |
subject_GND | (DE-588)4049722-7 (DE-588)4038971-6 (DE-588)4064937-4 (DE-588)4143413-4 |
title | Receptor-receptor interactions |
title_auth | Receptor-receptor interactions |
title_exact_search | Receptor-receptor interactions |
title_full | Receptor-receptor interactions ed. by P. Michael Conn |
title_fullStr | Receptor-receptor interactions ed. by P. Michael Conn |
title_full_unstemmed | Receptor-receptor interactions ed. by P. Michael Conn |
title_short | Receptor-receptor interactions |
title_sort | receptor receptor interactions |
topic | Rezeptor (DE-588)4049722-7 gnd Methode (DE-588)4038971-6 gnd Wechselwirkung (DE-588)4064937-4 gnd |
topic_facet | Rezeptor Methode Wechselwirkung Aufsatzsammlung |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026903774&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV002534878 |
work_keys_str_mv | AT connpatrickmichael receptorreceptorinteractions |