Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Garching
Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München e.V.
2013
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Selected topics of semiconductor physics and technology
170 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 5, 129 S. Ill., graph. Darst. |
| ISBN: | 9783941650701 |
Internformat
MARC
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| 245 | 1 | 0 | |a Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor |c Makiko Maruyama |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Garching |b Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München e.V. |c 2013 | |
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Datensatz im Suchindex
| _version_ | 1804151582165565440 |
|---|---|
| adam_text | CONTENTS
1 INTRODUCTION 1
2 FUNDAMENTALS 3
2.1 BIOSENSING TECHNIQUE 3
2.2 DNA AND PROTEINS 7
2.3 CHAINED MOLECULES AT THE METAL-SOLUTION INTERFACES 9
3 BINDING KINETICS OF SOLUTE TARGETS TO SURFACE BIOSENSORS IS
3.1 INTRODUCTION 15
3.2 INFLUENCE OF ANALYTE FLOW, DIFFUSION VS. CONVECTION 17
3.3 INFLUENCE OF THE TARGET-PROBE REACTION RATE: DIFFUSION VS. REACTION
20
3.4 THEORETICAL AND EXPERIMENTAL CONSIDERATIONS 25
3.5 CONCLUSIONS 30
4 DNA SENSING WITH ELECTRICALLY SWITCHABLE PNA INTERFACES 31
4.1 INTRODUCTION 31
4.2 SEQUENCE SELECTION 34
4.3 CHARACTERIZATION OF DNA-DNA/DNA-PNA BINDING IN SOLUTION 35
4.4 DNA/PNA LAYERS ON THE SWITCHSENSE SURFACE 42
4.5 HYBRIDIZATION UNDER STRINGENT CONDITIONS 52
4.6 DNA HYBRIDIZATION ON THE THE SPR BIOSENSOR 62
4.7 CONCLUSIONS 65
5 DETECTION AND SIZE ANALYSIS OF P53-DNA COMPLEX 67
5.1 INTRODUCTION 67
5.2 EXPERIMENTAL DESIGN 69
5.3 OBSERVATION OF P53-DNA INTERACTION ON THE SW/FCASENSE SENSOR 72
5.4 BINDING KINETICS OFP53 TO DNA 76
5.5 SIZING OF P53W/-DNA COMPLEX BY TIME-RESOLVED MEASUREMENTS 79
5.6 DNA CONFORMATION CHANGE AT VARIOUS P53WF CONCENTRATIONS 84
5.7 DISCUSSION 86
5.8 THE SPR BIOSENSOR MEASUREMENT 89
5.9 CONCLUSIONS 96
6 CONCLUSIONS 97
5
HTTP://D-NB.INFO/1044738979
7 MATERIAL AND METHODS 99
7.1 DNA SEQUENCES 99
7.2 PROTEIN 101
7.3 ANALYSIS IN SOLUTION-PHASE 102
7.4 EXPERIMENTAL COMPONENTS FOR THE JWIFC/ISENSE MEASUREMENT 103
7.5 SURFACE PREPARATION FOR THE JW/JC/ISENSE SENSOR 105
7.6 THIOLATED DNA IMMOBILIZATION IN THE SPR SENSOR 106
7.7 SURFACE REGENERATION IN THE SPR SENSOR 107
7.8 GEL ELECTROPHORESIS ANALYSIS FOR P53-DNA INTERACTION 107
A 109
A.L P53 GENE EXON 109
A.2 INFLUENCE OFDMF ON DNA-DNA HYBRIDIZATION 110
A.3 FLUORESCENCE CHANGE DUE TO P53 BINDING 110
A.4 ISOELECTRIC FOCUSING FOR P53 110
A.5 DNA HEIGHT CALCULATED BY THE MODEL AND MEASUREMENT 112
A.6 FITTING BY BIACORE SOFTWARE 113
REFERENCES 114
PUBLICATIONS 127
ACKNOWLEDGMENT 128
|
| any_adam_object | 1 |
| author | Maruyama, Makiko |
| author_facet | Maruyama, Makiko |
| author_role | aut |
| author_sort | Maruyama, Makiko |
| author_variant | m m mm |
| building | Verbundindex |
| bvnumber | BV041455501 |
| classification_tum | PHY 855d CHE 828d |
| ctrlnum | (OCoLC)867150213 (DE-599)BVBBV041455501 |
| dewey-full | 572.36 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.36 |
| dewey-search | 572.36 |
| dewey-sort | 3572.36 |
| dewey-tens | 570 - Biology |
| discipline | Physik Biologie Chemie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| id | DE-604.BV041455501 |
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| indexdate | 2024-07-10T00:57:06Z |
| institution | BVB |
| isbn | 9783941650701 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026901972 |
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| physical | 5, 129 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München e.V. |
| record_format | marc |
| series | Selected topics of semiconductor physics and technology |
| series2 | Selected topics of semiconductor physics and technology |
| spelling | Maruyama, Makiko Verfasser aut Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor Makiko Maruyama 1. Aufl. Garching Verein zur Förderung des Walter Schottky Inst. der Techn. Univ. München e.V. 2013 5, 129 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Selected topics of semiconductor physics and technology 170 Zugl.: München, Techn. Univ., Diss., 2013 Biosensor (DE-588)4193016-2 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Protein p53 (DE-588)4176550-3 gnd rswk-swf Bewegung (DE-588)4006311-2 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Protein p53 (DE-588)4176550-3 s Biochemische Analyse (DE-588)4255721-5 s Genanalyse (DE-588)4200230-8 s Biosensor (DE-588)4193016-2 s Bewegung (DE-588)4006311-2 s DE-604 Selected topics of semiconductor physics and technology 170 (DE-604)BV011499438 170 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026901972&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Maruyama, Makiko Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor Selected topics of semiconductor physics and technology Biosensor (DE-588)4193016-2 gnd Biochemische Analyse (DE-588)4255721-5 gnd Protein p53 (DE-588)4176550-3 gnd Bewegung (DE-588)4006311-2 gnd Genanalyse (DE-588)4200230-8 gnd |
| subject_GND | (DE-588)4193016-2 (DE-588)4255721-5 (DE-588)4176550-3 (DE-588)4006311-2 (DE-588)4200230-8 (DE-588)4113937-9 |
| title | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor |
| title_auth | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor |
| title_exact_search | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor |
| title_full | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor Makiko Maruyama |
| title_fullStr | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor Makiko Maruyama |
| title_full_unstemmed | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor Makiko Maruyama |
| title_short | Detection and analysis of the p53 gene and p53 protein with the switchSENSE biosensor |
| title_sort | detection and analysis of the p53 gene and p53 protein with the switchsense biosensor |
| topic | Biosensor (DE-588)4193016-2 gnd Biochemische Analyse (DE-588)4255721-5 gnd Protein p53 (DE-588)4176550-3 gnd Bewegung (DE-588)4006311-2 gnd Genanalyse (DE-588)4200230-8 gnd |
| topic_facet | Biosensor Biochemische Analyse Protein p53 Bewegung Genanalyse Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026901972&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV011499438 |
| work_keys_str_mv | AT maruyamamakiko detectionandanalysisofthep53geneandp53proteinwiththeswitchsensebiosensor |