Metabolite transporters in the chloroplast envelope:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-161859 Inhaltsverzeichnis |
| Beschreibung: | VIII, 88 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Jeshen, Ingrid Karin |d 1979- |e Verfasser |0 (DE-588)1043384863 |4 aut | |
| 245 | 1 | 0 | |a Metabolite transporters in the chloroplast envelope |c von Ingrid Karin Jeshen |
| 264 | 1 | |c 2012 | |
| 300 | |a VIII, 88 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2012 | ||
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| 856 | 4 | 1 | |u http://edoc.ub.uni-muenchen.de/16185/ |x Verlag |z kostenfrei |3 Volltext |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-026828616 | ||
Datensatz im Suchindex
| _version_ | 1804151476192280576 |
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| adam_text | TABLE OF CONTENTS
TABLE OF CONTENTS
SUMMARY I
ZUSAMMENFASSUNG II
TABLE OF CONTENTS IV
ABBREVIATIONS VII
I. INTRODUCTION 1
1 OEP24 3
2 0EP21 4
3 OTHER OEPS 4
4 AIM OF THE WORK 6
II. MATERIALS 7
1 CHEMICALS 7
2 ENZYMES 7
3 OLIGONUCLEOTIDES 7
4 VECTORS AND CONSTRUCTS 9
5 MOLECULAR WEIGHT MARKERS AND DNA STANDARDS 10
6 ANTISERA 10
7 STRAINS 10
8 PLANT MATERIAL 10
III. METHODS 12
1 PLANT METHODS 12
1.1 GROWTH OF ARABIDOPSIS THALIANA 12
1.2 CROSS FERTILIZATION OF ARABIDOPSIS THALIANA 12
1.3 STABLE TRANSFORMATION OF ARABIDOPSIS THALIANA 13
1.4 PREPARATION AND TRANSIENT TRANSFECTION OF ARABIDOPSIS THALIANA
PROTOPLASTS 13
2 MICROBIOLOGY METHODS 13
2.1 MEDIA AND GROWTH 13
2.2 BACTERIA TRANSFORMATION 14
3 MOLECULAR BIOLOGY METHODS 14
3.1 POLYMERASE CHAIN REACTION (PCR) 14
3.2 CLONING STRATEGIES 14
3.3 ISOLATION OF DNA PLASMIDS FROM ESCHERICHIA COLI 15
3.4 PREPARATION OF GENOMIC DNA FROM ARABIDOPSIS THALIANA 15
IV
HTTP://D-NB.INFO/1044675446
TABLE OF CONTENTS
3.5 DETERMINATION OF DNA AND RNA CONCENTRATIONS 16
3.6 CHARACTERISATION OF PLANT T-DNA INSERTION LINES 16
3.7 CHARACTERISATION OF PLANT TILLING LINES 18
3.8 DNA SEQUENCING 18
3.9 RNA EXTRACTION AND REAL TIME RT-PCR
;
18
4 BIOCHEMICAL METHODS 19
4.1 DETERMINATION OF PROTEIN CONCENTRATION 19
4.2 PROTEIN EXTRACTION FROM ARABIDOPSIS THALIANA 19
4.3 SDS-POLYACRYLAMIDE-GEL ELECTROPHORESIS (SDS-PAGE) 19
4.4 STAINING OF ACRYLAMIDE GELS 20
4.5 IMMUNODETECTION 20
4.6 GENERATION OF ANTISERA 21
4.7 PURIFICATION OF PSNOEP40 ANTISERUM 22
4.8 ISOELECTRIC FOCUSING (IEF) 23
4.9 PROTEIN IDENTIFICATION BY MASS SPECTROMETRY (MS) 23
4.10 HYDROPHOBICITY TEST 24
4.11 PROTEOLYSIS OF INNER ENVELOPE VESICLES FROM PISUM SATIVUM 24
4.12 PEGYLATION ASSAY 24
5 CELL BIOLOGY METHODS 24
5.1 PREPARATION OF INNER AND OUTER ENVELOPE VESICLES FROM PISUM SATIVUM
24
5.2 ISOLATION AND FRACTIONATION OF ARABIDOPSIS THALIANA CHLOROPLASTS 25
5.3 ISOLATION OF ARABIDOPSIS THALIANA CHLOROPLASTS 25
5.4 PREPARATION OF MICROSOMAL FRACTION FROM PISUM SATIVUM 25
6 METABOLITE ANALYSIS 26
7 MICROSCOPY 26
8 COMPUTATIONAL METHODS 27
IV. RESULTS 28
1 OEP24 IN ARABIDOPSIS 28
1.1 CHARACTERISATION OF OEP24.1 MUTANTS IN ARABIDOPSIS 28
1.2 CHARACTERISATION OF A MUTANT LINE FOR OEP24.2... 33
2 OEP21 IN ARABIDOPSIS 34
2.1 CHARACTERISATION OF OEP21 SINGLE MUTANTS IN ARABIDOPSIS 34
2.2 OEP21 DOUBLE MUTANTS 37
2.3 COMPLEMENTATION OF THE OEP21 DOUBLE MUTANT 38
2.4 STROMAL PROTEINS OF THE OEP21 DOUBLE MUTANT 38
2.5 METABOLITE ANALYSIS OF THE OEP21 DOUBLE MUTANT 39
V
TABLE OF CONTENTS
3 NEW MEMBRANE INTRINSIC PROTEINS IN THE CHLOROPLAST ENVELOPE ... 41
3.1 SUBCELLULAR LOCALISATION OF THE NEW ENVELOPE PROTEINS 45
3.2 MOLECULAR CHARACTERISATION OF PSNIEP57 48
3.3 MUTATION OFNOEP23 ANDNC)EP40 IN ARABIDOPSIS 52
3.4 IN PLANTA FUNCTION OF NIEP57 54
V. DISCUSSION 66
1 OEP24 66
2 0EP21 68
3 NEW CHLOROPLAST ENVELOPE PROTEINS 71
3.1 NOEP23 71
3.2 NOEP40 72
3.3 NIEP57 73
VI. OUTLOOK 76
VII. REFERENCE LIST 77
EIDESSTATTLICHE ERKLARUNG 87
ERKLARUNG 87
DANKSAGUNG 88
VI
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:55:25Z |
| institution | BVB |
| language | English |
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| physical | VIII, 88 Bl. Ill., graph. Darst. |
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| publishDateSearch | 2012 |
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| spelling | Jeshen, Ingrid Karin 1979- Verfasser (DE-588)1043384863 aut Metabolite transporters in the chloroplast envelope von Ingrid Karin Jeshen 2012 VIII, 88 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-161859 http://edoc.ub.uni-muenchen.de/16185/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-161859 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026828616&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Jeshen, Ingrid Karin 1979- Metabolite transporters in the chloroplast envelope |
| subject_GND | (DE-588)4113937-9 |
| title | Metabolite transporters in the chloroplast envelope |
| title_auth | Metabolite transporters in the chloroplast envelope |
| title_exact_search | Metabolite transporters in the chloroplast envelope |
| title_full | Metabolite transporters in the chloroplast envelope von Ingrid Karin Jeshen |
| title_fullStr | Metabolite transporters in the chloroplast envelope von Ingrid Karin Jeshen |
| title_full_unstemmed | Metabolite transporters in the chloroplast envelope von Ingrid Karin Jeshen |
| title_short | Metabolite transporters in the chloroplast envelope |
| title_sort | metabolite transporters in the chloroplast envelope |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/16185/ https://nbn-resolving.org/urn:nbn:de:bvb:19-161859 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026828616&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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