Microtubules, in vitro:
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2013
|
Ausgabe: | 2. ed. |
Schriftenreihe: | Methods in cell biology
115 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | 1. Aufl. u.d.T.: Microtubules, in vitro / Leslie Wilson |
Beschreibung: | XX, 417 S., [17] Bl. Ill., graph. Darst. |
ISBN: | 9780124077577 |
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264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2013 | |
300 | |a XX, 417 S., [17] Bl. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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---|---|
adam_text | Contents
Contributors
...........................................................................................................xiii
Preface
....................................................................................................................xix
CHAPTER
1
Site-Specific Fluorescent Labeling of Tubulin
............
ι
Kamalika
Mukherjee, Susan L. Bane
Introduction
..................................................................................2
1.1
Material
........................................................................................4
1.2
Method
..........................................................................................5
Acknowledgments
.....................................................................11
References
..................................................................................11
CHAPTER
2
Measuring Microtubule Persistence Length
Using a Microtubule Gliding Assay
................................
із
Douglas
S. Martin
Introduction
................................................................................14
2.1
Theory
........................................................................................15
2.2
Methods
......................................................................................17
2.3
Discussion
..................................................................................22
Summary
....................................................................................23
Acknowledgments
.....................................................................23
References
..................................................................................23
CHAPTER
3
Structural Studies of the Doublecortin
Family of MAPs
.....................................................................27
Franck
Fourniol,
Mylène
Perdenset, Anne Houdusse,
Carolyn
Moores
Introduction
................................................................................28
3.1
Rationale
.....................................................................................30
3.2
Methods
......................................................................................32
3.3
Discussion
..................................................................................43
Acknowledgments
.....................................................................44
References
..................................................................................45
CHAPTER
4
Detection and Quantification of Microtubule
Detachment from Centrosomes and
Spindle Poles
.........................................................................49
Anutosh Ganguly, Hailing Yang, Fernando
Cabral
Introduction
................................................................................50
4.1
Choice of Cell Line
....................................................................51
VI
Contents
CHAPTER
5
5.1
5.2
CHAPTER
6
CHAPTER
7
4.2
Microtubule Detachment
m
Spindles
........................................52
4.3
Labeling Microtubules
...............................................................53
4.4
Measuring Detachment
..............................................................54
4.5
Microtubule Fragments as a Surrogate for Detachment.
...........57
4.6
Mechanism of Detachment
........................................................59
Summary
....................................................................................60
Acknowledgments
.....................................................................60
References
..................................................................................60
Regulation of Tubulin Expression by Micro-RNAs:
Implications for Drug Resistance
...................................63
Sharon
Lobért,
Mary E. Graichen
Introduction and Rationale
.........................................................64
Methods and Materials
...............................................................65
Results and Discussion
..............................................................68
Summary
....................................................................................70
References
..................................................................................73
Determining the Structure-Mechanics
Relationships of Dense Microtubule Networks
with Confocal Microscopy and Magnetic
Tweezers-Based Microrheology
......................................75
Yali Yang, Megan T. Valentine
Introduction
................................................................................76
6.1
Protocols
.....................................................................................78
Summary
....................................................................................93
Acknowledgments
.....................................................................93
References
..................................................................................94
Studying Mitochondria and Microtubule
Localization and Dynamics in Standardized
Cell Shapes
.............................................................................97
Andrea Pelikan,
James Sillibourne, Stephanie Miserey-Lenkei,
Frederique Cariier-Grynkorn, Bruno
Goud,
Phong T.
Tran
Introduction
................................................................................98
7.1
Design of the Photomask
...........................................................99
7.2
Manufacturing of Micropatterned
Coverslips.........................100
7.3
Seeding and Spreading of Cells
...............................................101
7.4
Mitochondria and Microtubule Visualization
..........................102
7.5
Materials
...................................................................................104
Conclusion
................................................................................106
Acknowledgments
...................................................................107
References
................................................................................107
Contents
VII
CHAPTER
8
8.1
CHAPTER
9
9.1
9.2
9.3
9.4
CHAPTER
10
10.1
10.2
10.3
10.4
CHAPTER
11
11.1
11.2
Going Solo: Measuring the Motions
of Microtubules with an In Vitro Assay
for TIRF Microscopy
..........................................................109
Kris Leslie, Niels Galjart
Introduction
..............................................................................110
Protocols
...................................................................................
Ill
Concluding Remarks
...............................................................123
Acknowledgments
...................................................................123
References
................................................................................123
Analysis of Microtubules in Isolated
Axoplasm from the Squid Giant Axon
..........................125
Yuyu Song, Scott T. Brady
Preparation of Axoplasm
.........................................................126
Analysis of Axoplasmic MicrotubuJe Dynamics
....................131
Biochemistry of Axoplasmic Microtubules
.............................133
Immunohistochemistry of Axoplasmic Microtubules
.............135
References
................................................................................136
Imaging GTP-Bound Tubulin: From Cellular
to In Vitro Assembled Microtubules
............................139
Hélène de
Forges,
Antoine Pilon,
Christian
Poüs,
Franck
Perez
Introduction
..............................................................................141
Imaging GTP Islands
m
Permeabilrzed Cells
.........................142
Imaging GTP Caps and GTP Islands Using
Centxosome-Based Microtubule Assembly or Endogenous
Microtubule Elongation in Permeabilized Cells
.....................145
Imaging GTP Islands
m
Microtubules Assembled
In Vitro
.....................................................................................147
Discussion and Future Prospects
.............................................150
Acknowledgments
...................................................................151
References
................................................................................152
Tubulin-Specific Chaperones: Components
of a Molecular Machine That Assembles
the
α/β
Heterodimer
.........................................................155
Guoling
Tian,
Nicholas J. Cowan
Introduction
..............................................................................156
Methods
....................................................................................159
Discussion
................................................................................167
References
................................................................................169
VIII
Contents
CHAPTER
12
Heterotrimeric
G
Proteins and Microtubules
............173
Witchuda Saengsawang, Mark M.
Rasenick
Introduction
..............................................................................174
12.1
General Protocols
.....................................................................174
12.2
Buffer Compositions
................................................................187
Concluding Comments
............................................................188
Acknowledgments
...................................................................188
References
................................................................................188
CHAPTER
13
Purification and Biophysical Analysis of
Microtubule-Severing Enzymes In Vitro
.....................191
Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross
Introduction
..............................................................................192
13.1
Methods
....................................................................................194
Discussion and Summary
........................................................211
Acknowledgments
...................................................................211
References
................................................................................211
CHAPTER
14
Measurement of In Vitro Microtubule
Polymerization by Turbidity and Fluorescence
215
Matthew Mirigian,
Kamalika
Mukherjee, Susan L. Bane,
Dan L. Sackett
14.1
Background and Theory
...........................................................216
14.2
Materials and Equipment
.........................................................223
14.3
Methods
....................................................................................225
References
.................................................................................228
CHAPTER
15
Live-Cell Imaging of Microtubules and
Microtubule-Associated Proteins
in Arabidopsis thaliana
....................................................231
Jessica Lucas
Introduction
..............................................................................232
15.1
Protocols
...................................................................................234
15.2
Matenals
...................................................................................242
Concluding Comments
............................................................243
Acknowledgment
.....................................................................243
References
................................................................................243
CHAPTER
16
Investigating Tubulin Posttranslational
Modifications with Specific Antibodies
.....................247
Maria M.
Magiéra,
Carsten Janke
Introduction
..............................................................................248
16.1
Observations
.............................................................................249
Contents
IX
CHAPTER
17
CHAPTER
18
CHAPTER
19
16.2
Matenals
and Methods
.............................................................255
16.3
Buffer Composition
...................................................................261
Concluding Remarks
...............................................................263
Acknowledgments
...................................................................263
References
................................................................................264
Purification and Assembly of Bacterial Tubulin
BtubA/B and Constructs Bearing Eukaryotic
Tubulin Sequences
............................................................269
José M.
Andreu,
María A. Oliva
Introduction
and Rationale
.......................................................270
17.1
Matenals:
Genes, Constructs, and Expressed Proteins
...........272
17.2
Methods
....................................................................................273
Conclusions
..............................................................................280
Acknowledgments
...................................................................280
References
................................................................................280
Microtubule-Associated Proteins and
Tubulin Interaction by Isothermal
Titration
Calorimetry
...........................................................................283
P.
0.
Tsvetkov, P.
Barbier, G.
Breuzard, V. Peyrot, F. Devred
Introduction
..............................................................................284
18.1
Isothermal
Titration Calonmetry.............................................286
18.2
Tubulin and MAPs Sample Preparation
..................................290
18.3
Results: Tubulm/MAPs by
ITC...............................................294
Conclusion
.................................................................................298
Acknowledgment
.....................................................................299
References
................................................................................299
Methods for Studying Microtubule Binding
Site Interactions: Zampanolide as a Covalent
Binding Agent
.......................................................................303
Jessica J. Field,
Enrique
Calvo,
Peter
T.
Northcote,
John H. Miller,
Karl-Heinz Altmann,
José
Fernando
Díaz
Introduction
..............................................................................304
19.1
Matenals
...................................................................................305
19.2
Methods and Results
................................................................307
Summary
..................................................................................324
Acknowledgments
...................................................................324
References
................................................................................324
Contents
CHAPTER
20
20.1
20.2
20.3
CHAPTER
21
21.1
21.2
21.3
CHAPTER
22
22.1
22.2
22.3
22.4
22.5
22.6
CHAPTER
23
23.1
23.2
23.3
23.4
Studying Kinetochore-Fiber
Ultrastructure
Using Correlative Light-Electron Microscopy
..........327
Daniel G. Booth,
Liam
P. Cheeseman, Ian A. Prior,
Stephen J. Royle
Introduction
..............................................................................328
Matenals
...................................................................................329
Methods
....................................................................................331
Discussion
................................................................................338
Acknowledgments
...................................................................341
References
................................................................................341
Fluorescence-Based Assays for Microtubule
Architecture
..........................................................................343
Susanne
Bechstcdt, Gary J. Brouhard
Introduction
..............................................................................344
Matenals
...................................................................................347
Methods
....................................................................................348
Discussion
................................................................................352
Acknowledgments
...................................................................352
References
................................................................................352
Structure-Function Analysis of Yeast Tubulin
.........355
Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta
Introduction
..............................................................................356
Reagents and Equipment
.........................................................358
Introducing Tubulin Mutations into Yeast
..............................360
Analysis of Tetrad Viability
....................................................363
Assessing Microtubule Stability by Drug Sensitivity
.............366
Direct Analysis of Microtubule Dynamics In Vivo
.................367
Localization of Microtubule-Associated Proteins In Vivo
......370
Acknowledgments
...................................................................372
References
................................................................................373
Using MTBindingSim as a Tool for Experimental
Planning and Interpretation
............................................375
Julia T. Philip, Aranda R. Duan, Emily
0.
Alberico,
Holly V. Goodson
Introduction
..............................................................................376
MTBmdmgSim
.........................................................................376
Expenmental Designs in MTBmdmgSim
...............................376
Binding Models in MTBmdmgSim
.........................................377
Using MTBmdmgSim Example
1:
Tau-MT Binding
............379
Contents xi
23.5
Using MTBindingSira Example
2:
Does Human
RBI Bind to the MT Seam or Lattice?
...................................381
Conclusion
................................................................................383
References
................................................................................383
CHAPTER
24
Imaging Individual Spindle Microtubule
Dynamics in Fission Yeast
..............................................385
Judite
Costa, Chuanhai
Fu,
Viktonya Syrovatkina,
Phong T.
Tran
Introduction
..............................................................................386
24.1
Methods
....................................................................................387
Conclusion
................................................................................393
Acknowledgments
...................................................................393
References
................................................................................394
Index
.......................................................................................................................395
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spelling | Microtubules, in vitro ed. by John J. Correia ... 2. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2013 XX, 417 S., [17] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 115 1. Aufl. u.d.T.: Microtubules, in vitro / Leslie Wilson Mikrotubulus (DE-588)4169864-2 gnd rswk-swf Mikrotubulus (DE-588)4169864-2 s DE-604 Correia, John Joseph. Sonstige oth Methods in cell biology 115 (DE-604)BV002534878 115 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026800261&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Microtubules, in vitro Methods in cell biology Mikrotubulus (DE-588)4169864-2 gnd |
subject_GND | (DE-588)4169864-2 |
title | Microtubules, in vitro |
title_auth | Microtubules, in vitro |
title_exact_search | Microtubules, in vitro |
title_full | Microtubules, in vitro ed. by John J. Correia ... |
title_fullStr | Microtubules, in vitro ed. by John J. Correia ... |
title_full_unstemmed | Microtubules, in vitro ed. by John J. Correia ... |
title_short | Microtubules, in vitro |
title_sort | microtubules in vitro |
topic | Mikrotubulus (DE-588)4169864-2 gnd |
topic_facet | Mikrotubulus |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026800261&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV002534878 |
work_keys_str_mv | AT correiajohnjoseph microtubulesinvitro |