In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Köster
2013
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Schriften zur Internationalen Agrarentwicklung
66 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 184 S. Ill., graph. Darst., Kt. |
| ISBN: | 9783895748318 |
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Datensatz im Suchindex
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|---|---|
| adam_text | Titel: In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivar
Autor: Ibraheem, Yamen
Jahr: 2013
Table of contents
Zusammenfassung.........................................................................................................................................I
Abstract...............................................................................................____.................................................... III
Abbreviations
1. Introduction...............................................................................................................................................1
2. Literature review.......................................................................................................................................5
2.1. Botanical characteristics, growing conditions and economical importance of Date Palm...................S
2.2. Propagation methods..........................................................................................................................12
2.2.1. Traditional methods....................................................................................................................12
2.2.2. Propagation of date palm using tissue culture techniques..........................................................13
2.2.2.1. Preparation and disinfection of seeds and shoot tips.....................................................14
2.2.2.2. Organogenesis of date palm..........................................................................................16
2.2.2.3. Somatic embryogenesis of date palm............................................................................17
2.2.2.4. Callus induction from various expiants.........................................................................18
2.2.2.5. Somatic embryo induction............................................................................................22
2.2.2.6. Somatic embryo conversion..........................................................................................23
2.2.2.7. A comparative study between solid and liquid cultures relative to callus growth and
somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool........................24
2.2.2.8. Elongation.....................................................................................................................27
2.2.2.9. Rooting..........................................................................................................................28
2.2.3. Acclimatization..........................................................................................................................29
2.3 Salt stress............................................................................................................................................30
2.3.1. Using tissue culture techniques for selecting tolerant cultivais..................................................31
2.3.2. The effect of high salt concentration on somatic embryogenesis...............................................34
3. Aims and objectives................................................................................................................................. 35
3.1. Research aims....................................................................................................................................35
3.2. Specific objectives.............................................................................................................................35
3.3. Hypothesis.........................................................................................................................................36
4. Material and Methods.....«...............................................«---------------------------------------------------------38
IV
4.1. Plant materials....................................................................................................................................38
4.2. Preparation and disinfection...............................................................................................................42
4.2.1. Seeds and zygotic embryos........................................................................................................42
4.2.2. Shoot tips....................................................................................................................................44
4.3. Nutrient medium................................................................................................................................45
4.4. Culture conditions..............................................................................................................................45
4.5. Callus induction.................................................................................................................................47
4.6. Somatic embiyo formation.................................................................................................................48
4.7. A comparative study between solid and liquid cultures relative to callus growth and somatic
embiyo formation in date palm cv. Zaghlool............................................................................................49
4.7.1. The effect of culture system and medium composition on callus growth..................................49
4.7.2. The effect of culture system and medium composition on somatic embryo formation..............51
4.8. Conversion of somatic embiyos, elongation and multiplication.......................................................52
4.8.1. Somatic embryo conversion.......................................................................................................52
4.8.2. A comparative study between somatic embryo conversion and germination of zygotic
embryos and seeds of cv. Zaghlool......................................................................................................53
4.8.3. Indirect shoot regeneration, elongation and multiplication........................................................53
4.9. Rooting...............................................................................................................................................54
4.9.1. Effect of auxin type and concentration.......................................................................................54
4.9.2. Effect of solidifying agent..........................................................................................................54
4.9.3. Effect ofNAA concentration.....................................................................................................54
4.9.4. Effect of PEG on in vitro rooting...............................................................................................55
4.10. Data analysis....................................................................................................................................55
4.11. Acclimatization................................................................................................................................56
4.11.1. The effect of plant height on plantlet survival after transfer to ex vitro conditions.................57
4.11.2. The effect of rooting medium on plantlet survival after transfer to ex vitro conditions...........57
4.11.3. The effect of acclimatization substrate and rooting medium on plantlet survival....................58
4. II .4. The effect of polyethylene glycol on plantlet survival.............................................................58
4.12. In vitro studies of salt stress on date palm.......................................................................................59
4.12.1. The effect of sodium chloride-stress on date palm somatic embryogenesis.............................59
4.12.2. Screening of some date palm cultivars to salt stress in vitro....................................................60
5. Results and discussion ..........................................................................................................................62
V
5.1. Preparation and disinfection...............................................................................................................63
5.1.1. Seeds and zygotic embryos........................................................................................................63
5.1.2. Shoot tips....................................................................................................................................66
5.2. Callus induction.................................................................................................................................67
5.2.1. Zygotic embiyos.........................................................................................................................67
5.2.2. Shoot tips....................................................................................................................................70
5.2.3. Seedling segments......................................................................................................................73
5.2.4. Discussion..................................................................................................................................77
5.2.4.1. Effect of expiant source and type..................................................................................77
5.2.4.2. Effect of auxin...............................................................................................................79
5.2.4.3. Effect of genotype.........................................................................................................80
5.3. Somatic embryo formation.................................................................................................................81
5.3.1. Start material: zygotic embryos..................................................................................................81
5.3.2. Start material: shoot tips.............................................................................................................83
5.3.3. Start material: leaf segments......................................................................................................87
5.3.4. Discussion..................................................................................................................................87
5.3.4.1. Effect of expiants..........................................................................................................87
5.3.4.2. Effect of auxin and cytokinin........................................................................................88
5.4. A comparative study between solid and liquid cultures relative to callus growth and somatic
embryo formation in date palm cv. Zaghlool............................................................................................90
5.4.1. The effect of culture system and medium composition on callus growth..................................90
5.4.2. Somatic embryo formation.........................................................................................................94
5.4.2.1. During the course of liquid medium.............................................................................94
5.4.2.2. After transfer the calli to somatic embryo formation medium......................................96
5.4.3. The effect of culture system and medium composition on somatic embryo formation............100
5.4.3.1. Callus induced from shoot tips....................................................................................100
5.4.3.2. Callus induced from leaflet segments.........................................................................104
5.5. Conversion of somatic embryos» elongation and multiplication......................................................107
5.5.1. Conversion of somatic embiyos...............................................................................................107
5.5.2. A comparative study between somatic embryo conversion and germination of zygotic
embryos and seeds of cv. Zaghlool....................................................................................................112
5.5.3. Elongation of shoots and multiplication...................................................................................115
VI
5.5.3.1. Indirect shoot regeneration..........................................................................................115
5.5.3.2. Elongation and multiplication.....................................................................................117
5.6. Rooting.............................................................................................................................................119
5.6.1. Effect of auxin type and concentration.....................................................................................119
5.6.2. Effect of solidifying agent........................................................................................................121
5.6.3. Effect of NAA concentration...................................................................................................123
5.6.4 Effect of PEG on rooting...........................................................................................................124
5.7. Acclimatization................................................................................................................................126
5.7.1. The effect of the plant height on the acclimatization...............................................................126
5.7.2. The effect of rooting medium on plantlet survival ex vitro......................................................126
5.7.3. The effect of acclimatization substrate and rooting medium on acclimatization.....................126
5.7.4. The effect of polyethylene glycol on plantlet survival rate ex vitro.........................................130
5.7.5. Plantlet development after acclimatization.............................................................................132
5.8. In vitro studies of salt stress on date palm.......................................................................................134
5.8.1. The effect of sodium chloride-stress on date palm cv. Zaghlool somatic embryogenesis........134
5.8.1.1. The effect ofNaCl concentration on callus growth.....................................................134
5.8.1.2. The effect ofNaCl concentration on somatic embryo formation................................138
5.8.1.3. The effect of NaCl on conversion of date palm somatic embiyos..............................139
5.8.2. Screening of some date palm cultivars to salt stress in vitro....................................................141
6. Conclusions..__________.......__________........________.........._______......__________.......__________......__________......____________.....______149
7. References....._________.........._______............____.............____......__________.......__________......------------.....___________....--------151
Index of figures_________.............____............____.......__________......___________......__________......_____________....__________......-------180
List of tables....__________......_________......._________.......__________......___________.....___________......___________......__________......_______183
VII
|
| any_adam_object | 1 |
| author | Ibraheem, Yamen 1980- |
| author_GND | (DE-588)1042584168 |
| author_facet | Ibraheem, Yamen 1980- |
| author_role | aut |
| author_sort | Ibraheem, Yamen 1980- |
| author_variant | y i yi |
| building | Verbundindex |
| bvnumber | BV041342363 |
| ctrlnum | (OCoLC)915260469 (DE-599)DNB1038072387 |
| dewey-full | 634.623 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 634 - Orchards, fruits, forestry |
| dewey-raw | 634.623 |
| dewey-search | 634.623 |
| dewey-sort | 3634.623 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | Ibraheem, Yamen 1980- Verfasser (DE-588)1042584168 aut In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars von Yamen Ibraheem 1. Aufl. Berlin Köster 2013 XI, 184 S. Ill., graph. Darst., Kt. txt rdacontent n rdamedia nc rdacarrier Schriften zur Internationalen Agrarentwicklung 66 Zugl.: Berlin, Humboldt-Univ., Diss., 2013 Dattelpalme (DE-588)4142068-8 gnd rswk-swf Vegetative Vermehrung (DE-588)4137214-1 gnd rswk-swf Syrien (DE-588)4058794-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Syrien (DE-588)4058794-0 g Dattelpalme (DE-588)4142068-8 s Vegetative Vermehrung (DE-588)4137214-1 s DE-604 Schriften zur Internationalen Agrarentwicklung 66 (DE-604)BV008281241 66 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026791109&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ibraheem, Yamen 1980- In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars Schriften zur Internationalen Agrarentwicklung Dattelpalme (DE-588)4142068-8 gnd Vegetative Vermehrung (DE-588)4137214-1 gnd |
| subject_GND | (DE-588)4142068-8 (DE-588)4137214-1 (DE-588)4058794-0 (DE-588)4113937-9 |
| title | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars |
| title_auth | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars |
| title_exact_search | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars |
| title_full | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars von Yamen Ibraheem |
| title_fullStr | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars von Yamen Ibraheem |
| title_full_unstemmed | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars von Yamen Ibraheem |
| title_short | In vitro regeneration systems for economically important date palm (Phoenix dactylifera L.) cultivars |
| title_sort | in vitro regeneration systems for economically important date palm phoenix dactylifera l cultivars |
| topic | Dattelpalme (DE-588)4142068-8 gnd Vegetative Vermehrung (DE-588)4137214-1 gnd |
| topic_facet | Dattelpalme Vegetative Vermehrung Syrien Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026791109&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV008281241 |
| work_keys_str_mv | AT ibraheemyamen invitroregenerationsystemsforeconomicallyimportantdatepalmphoenixdactyliferalcultivars |