Rapid development of small-molecule producing microorganisms based on metabolite sensors:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Jülich
Forschungszentrum Jülich, Zentralbibliothek
2013
|
| Series: | Schriften des Forschungszentrums Jülich
Reihe Gesundheit ; 65 |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | 138 S. Ill., graph. Darst. |
| ISBN: | 9783893368723 |
Staff View
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Record in the Search Index
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| adam_text | CONTENT
CONTENT
1.1. SUMMARY 1
1.2. ZUSAMMENFASSUNG 2
2. INTRODUCTION 4
2.1. FERMENTATIVE PRODUCTION OF SMALL-MOLECULES 4
2.1.1. INDUSTRIAL RELEVANCE OF L-LYSINE AND OTHER AMINO ACIDS 4
2.1.2. BIOSYNTHESIS AND PRODUCTION OF L-LYSINE BY CORYNEBACTERIUM
GLUTAMICUM 5
2.2. DEVELOPMENT OF SMALL-MOLECULE PRODUCING BACTERIA 8
2.2.1. DIVERSITY GENERATION 8
2.2.2. SCREENING FOR SMALL-MOLECULES 10
2.3. GENETIC TOOLS FOR GENOME ENGINEERING 12
2.3.1. HOMOLOGOUS RECOMBINATION USING NON-REPLICATIVE INTEGRATION
VECTORS 12
2.3.2. RECOMBINATION-MEDIATED GENETIC ENGINEERING (RECOMBINEERING) 12
2.4. AIMS OF THIS WORK 14
3. RESULTS 15
3.1. A HIGH-THROUGHPUT APPROACH TO IDENTIFY GENOMIC VARIANTS OF
BACTERIAL METABOLITE
PRODUCERS AT THE SINGLE-CELL LEVEL 15
3.2. RECOMBINEERING IN CORYNEBACTERIUM GLUTAMICUM COMBINED WITH OPTICAL
NANOSENSORS: A GENERAL STRATEGY FOR FAST PRODUCER STRAIN GENERATION 27
3.3. DEVELOPMENT OF A BIOSENSOR FOR THE DETECTION OF LOW INTRACELLULAR
NADPH/NADP*
RATIOS AND ITS APPLICATION FOR ENZYME EVOLUTION AND HT-SCREENING 36
4. DISCUSSION 40
4.1. NANOSENSORS FOR ANALYSING INTRACELLULAR METABOLITE CONCENTRATIONS
40
4.2. SCREENING AND ISOLATION OF NEW L-LYSINE PRODUCING STRAINS AFTER
MUTAGENESIS 42
4.3. IDENTIFICATION OF NEW MUTATIONS BY WHOLE-GENOME SEQUENCING 44
4.4. RECOMBINEERING IN CORYNEBACTERIUM GLUTAMICUM 47
4.5. RECOMBINEERING AND NANOSENSORS: RAPID DEVELOPMENT OF SMALL-MOLECULE
PRODUCING STRAINS 49
4.6. A NEW CLASS OF NANOSENSORS: DETECTION OF CYTOSOLIC NADPH/NADP
+
LEVELS 52
5. REFERENCES 54
HTTP://D-NB.INFO/1036563081
CONTENT
6. APPENDIX 62
6.1. SUPPLEMENTARY MATERIAL A HIGH-THROUGHPUT APPROACH TO IDENTIFY
GENOMIC
VARIANTS OF BACTERIAL METABOLITE PRODUCERS AT THE SINGLE-CELL LEVEL 62
6.2. SUPPLEMENTARY MATERIAL RECOMBINEERING IN CORYNEBACTERIUM
GLUTAMICUM COMBINED
WITH OPTICAL NANOSENSORS: A GENERAL STRATEGY FOR FAST PRODUCER STRAIN
GENERATION 80
6.3. WHOLE-GENOME SEQUENCING: STATISTICAL DATA FOR TEN SELECTED C.
GLUTAMICUM STRAINS 84
|
| any_adam_object | 1 |
| author | Binder, Stephan |
| author_GND | (DE-588)1037121384 |
| author_facet | Binder, Stephan |
| author_role | aut |
| author_sort | Binder, Stephan |
| author_variant | s b sb |
| building | Verbundindex |
| bvnumber | BV041297682 |
| ctrlnum | (OCoLC)862805170 (DE-599)DNB1036563081 |
| dewey-full | 660.65 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.65 |
| dewey-search | 660.65 |
| dewey-sort | 3660.65 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Thesis Book |
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| id | DE-604.BV041297682 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:53:39Z |
| institution | BVB |
| isbn | 9783893368723 |
| language | English |
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| physical | 138 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
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| publisher | Forschungszentrum Jülich, Zentralbibliothek |
| record_format | marc |
| series | Schriften des Forschungszentrums Jülich |
| series2 | Schriften des Forschungszentrums Jülich : Reihe Gesundheit |
| spelling | Binder, Stephan Verfasser (DE-588)1037121384 aut Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder Jülich Forschungszentrum Jülich, Zentralbibliothek 2013 138 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Schriften des Forschungszentrums Jülich : Reihe Gesundheit 65 Zugl.: Düsseldorf, Univ., Diss., 2012 Lysin (DE-588)4168397-3 gnd rswk-swf Metabolit (DE-588)4138579-2 gnd rswk-swf Corynebacterium glutamicum (DE-588)4309351-6 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf Genomik (DE-588)4776397-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biosensor (DE-588)4193016-2 s Metabolit (DE-588)4138579-2 s Corynebacterium glutamicum (DE-588)4309351-6 s Lysin (DE-588)4168397-3 s Genomik (DE-588)4776397-8 s DE-604 Schriften des Forschungszentrums Jülich Reihe Gesundheit ; 65 (DE-604)BV035800060 65 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026746532&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Binder, Stephan Rapid development of small-molecule producing microorganisms based on metabolite sensors Schriften des Forschungszentrums Jülich Lysin (DE-588)4168397-3 gnd Metabolit (DE-588)4138579-2 gnd Corynebacterium glutamicum (DE-588)4309351-6 gnd Biosensor (DE-588)4193016-2 gnd Genomik (DE-588)4776397-8 gnd |
| subject_GND | (DE-588)4168397-3 (DE-588)4138579-2 (DE-588)4309351-6 (DE-588)4193016-2 (DE-588)4776397-8 (DE-588)4113937-9 |
| title | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_auth | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_exact_search | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_full | Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder |
| title_fullStr | Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder |
| title_full_unstemmed | Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder |
| title_short | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_sort | rapid development of small molecule producing microorganisms based on metabolite sensors |
| topic | Lysin (DE-588)4168397-3 gnd Metabolit (DE-588)4138579-2 gnd Corynebacterium glutamicum (DE-588)4309351-6 gnd Biosensor (DE-588)4193016-2 gnd Genomik (DE-588)4776397-8 gnd |
| topic_facet | Lysin Metabolit Corynebacterium glutamicum Biosensor Genomik Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026746532&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035800060 |
| work_keys_str_mv | AT binderstephan rapiddevelopmentofsmallmoleculeproducingmicroorganismsbasedonmetabolitesensors |