Rapid development of small-molecule producing microorganisms based on metabolite sensors:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Jülich
Forschungszentrum Jülich, Zentralbibliothek
2013
|
| Schriftenreihe: | Schriften des Forschungszentrums Jülich
Reihe Gesundheit ; 65 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 138 S. Ill., graph. Darst. |
| ISBN: | 9783893368723 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV041297682 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 131001s2013 ad|| m||| 00||| eng d | ||
| 015 | |a 13N27 |2 dnb | ||
| 016 | 7 | |a 1036563081 |2 DE-101 | |
| 020 | |a 9783893368723 |c : Pb. : EUR 26.00 (DE) |9 978-3-89336-872-3 | ||
| 035 | |a (OCoLC)862805170 | ||
| 035 | |a (DE-599)DNB1036563081 | ||
| 040 | |a DE-604 |b ger |e aacr | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-12 | ||
| 082 | 0 | |a 660.65 |2 22/ger | |
| 084 | |a 660 |2 sdnb | ||
| 100 | 1 | |a Binder, Stephan |e Verfasser |0 (DE-588)1037121384 |4 aut | |
| 245 | 1 | 0 | |a Rapid development of small-molecule producing microorganisms based on metabolite sensors |c Stephan Binder |
| 264 | 1 | |a Jülich |b Forschungszentrum Jülich, Zentralbibliothek |c 2013 | |
| 300 | |a 138 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Schriften des Forschungszentrums Jülich : Reihe Gesundheit |v 65 | |
| 502 | |a Zugl.: Düsseldorf, Univ., Diss., 2012 | ||
| 650 | 0 | 7 | |a Lysin |0 (DE-588)4168397-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Metabolit |0 (DE-588)4138579-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Corynebacterium glutamicum |0 (DE-588)4309351-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biosensor |0 (DE-588)4193016-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genomik |0 (DE-588)4776397-8 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Biosensor |0 (DE-588)4193016-2 |D s |
| 689 | 0 | 1 | |a Metabolit |0 (DE-588)4138579-2 |D s |
| 689 | 0 | 2 | |a Corynebacterium glutamicum |0 (DE-588)4309351-6 |D s |
| 689 | 0 | 3 | |a Lysin |0 (DE-588)4168397-3 |D s |
| 689 | 0 | 4 | |a Genomik |0 (DE-588)4776397-8 |D s |
| 689 | 0 | |5 DE-604 | |
| 830 | 0 | |a Schriften des Forschungszentrums Jülich |v Reihe Gesundheit ; 65 |w (DE-604)BV035800060 |9 65 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026746532&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-026746532 | ||
Datensatz im Suchindex
| _version_ | 1804151365160665088 |
|---|---|
| adam_text | CONTENT
CONTENT
1.1. SUMMARY 1
1.2. ZUSAMMENFASSUNG 2
2. INTRODUCTION 4
2.1. FERMENTATIVE PRODUCTION OF SMALL-MOLECULES 4
2.1.1. INDUSTRIAL RELEVANCE OF L-LYSINE AND OTHER AMINO ACIDS 4
2.1.2. BIOSYNTHESIS AND PRODUCTION OF L-LYSINE BY CORYNEBACTERIUM
GLUTAMICUM 5
2.2. DEVELOPMENT OF SMALL-MOLECULE PRODUCING BACTERIA 8
2.2.1. DIVERSITY GENERATION 8
2.2.2. SCREENING FOR SMALL-MOLECULES 10
2.3. GENETIC TOOLS FOR GENOME ENGINEERING 12
2.3.1. HOMOLOGOUS RECOMBINATION USING NON-REPLICATIVE INTEGRATION
VECTORS 12
2.3.2. RECOMBINATION-MEDIATED GENETIC ENGINEERING (RECOMBINEERING) 12
2.4. AIMS OF THIS WORK 14
3. RESULTS 15
3.1. A HIGH-THROUGHPUT APPROACH TO IDENTIFY GENOMIC VARIANTS OF
BACTERIAL METABOLITE
PRODUCERS AT THE SINGLE-CELL LEVEL 15
3.2. RECOMBINEERING IN CORYNEBACTERIUM GLUTAMICUM COMBINED WITH OPTICAL
NANOSENSORS: A GENERAL STRATEGY FOR FAST PRODUCER STRAIN GENERATION 27
3.3. DEVELOPMENT OF A BIOSENSOR FOR THE DETECTION OF LOW INTRACELLULAR
NADPH/NADP*
RATIOS AND ITS APPLICATION FOR ENZYME EVOLUTION AND HT-SCREENING 36
4. DISCUSSION 40
4.1. NANOSENSORS FOR ANALYSING INTRACELLULAR METABOLITE CONCENTRATIONS
40
4.2. SCREENING AND ISOLATION OF NEW L-LYSINE PRODUCING STRAINS AFTER
MUTAGENESIS 42
4.3. IDENTIFICATION OF NEW MUTATIONS BY WHOLE-GENOME SEQUENCING 44
4.4. RECOMBINEERING IN CORYNEBACTERIUM GLUTAMICUM 47
4.5. RECOMBINEERING AND NANOSENSORS: RAPID DEVELOPMENT OF SMALL-MOLECULE
PRODUCING STRAINS 49
4.6. A NEW CLASS OF NANOSENSORS: DETECTION OF CYTOSOLIC NADPH/NADP
+
LEVELS 52
5. REFERENCES 54
HTTP://D-NB.INFO/1036563081
CONTENT
6. APPENDIX 62
6.1. SUPPLEMENTARY MATERIAL A HIGH-THROUGHPUT APPROACH TO IDENTIFY
GENOMIC
VARIANTS OF BACTERIAL METABOLITE PRODUCERS AT THE SINGLE-CELL LEVEL 62
6.2. SUPPLEMENTARY MATERIAL RECOMBINEERING IN CORYNEBACTERIUM
GLUTAMICUM COMBINED
WITH OPTICAL NANOSENSORS: A GENERAL STRATEGY FOR FAST PRODUCER STRAIN
GENERATION 80
6.3. WHOLE-GENOME SEQUENCING: STATISTICAL DATA FOR TEN SELECTED C.
GLUTAMICUM STRAINS 84
|
| any_adam_object | 1 |
| author | Binder, Stephan |
| author_GND | (DE-588)1037121384 |
| author_facet | Binder, Stephan |
| author_role | aut |
| author_sort | Binder, Stephan |
| author_variant | s b sb |
| building | Verbundindex |
| bvnumber | BV041297682 |
| ctrlnum | (OCoLC)862805170 (DE-599)DNB1036563081 |
| dewey-full | 660.65 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.65 |
| dewey-search | 660.65 |
| dewey-sort | 3660.65 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02092nam a2200493 cb4500</leader><controlfield tag="001">BV041297682</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">131001s2013 ad|| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">13N27</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1036563081</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783893368723</subfield><subfield code="c">: Pb. : EUR 26.00 (DE)</subfield><subfield code="9">978-3-89336-872-3</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)862805170</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1036563081</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">aacr</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">660.65</subfield><subfield code="2">22/ger</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">660</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Binder, Stephan</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1037121384</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Rapid development of small-molecule producing microorganisms based on metabolite sensors</subfield><subfield code="c">Stephan Binder</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Jülich</subfield><subfield code="b">Forschungszentrum Jülich, Zentralbibliothek</subfield><subfield code="c">2013</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">138 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Schriften des Forschungszentrums Jülich : Reihe Gesundheit</subfield><subfield code="v">65</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Zugl.: Düsseldorf, Univ., Diss., 2012</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Lysin</subfield><subfield code="0">(DE-588)4168397-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Metabolit</subfield><subfield code="0">(DE-588)4138579-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Corynebacterium glutamicum</subfield><subfield code="0">(DE-588)4309351-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biosensor</subfield><subfield code="0">(DE-588)4193016-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genomik</subfield><subfield code="0">(DE-588)4776397-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Biosensor</subfield><subfield code="0">(DE-588)4193016-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Metabolit</subfield><subfield code="0">(DE-588)4138579-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Corynebacterium glutamicum</subfield><subfield code="0">(DE-588)4309351-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Lysin</subfield><subfield code="0">(DE-588)4168397-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="4"><subfield code="a">Genomik</subfield><subfield code="0">(DE-588)4776397-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Schriften des Forschungszentrums Jülich</subfield><subfield code="v">Reihe Gesundheit ; 65</subfield><subfield code="w">(DE-604)BV035800060</subfield><subfield code="9">65</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026746532&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-026746532</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV041297682 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:53:39Z |
| institution | BVB |
| isbn | 9783893368723 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026746532 |
| oclc_num | 862805170 |
| open_access_boolean | |
| owner | DE-12 |
| owner_facet | DE-12 |
| physical | 138 S. Ill., graph. Darst. |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Forschungszentrum Jülich, Zentralbibliothek |
| record_format | marc |
| series | Schriften des Forschungszentrums Jülich |
| series2 | Schriften des Forschungszentrums Jülich : Reihe Gesundheit |
| spelling | Binder, Stephan Verfasser (DE-588)1037121384 aut Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder Jülich Forschungszentrum Jülich, Zentralbibliothek 2013 138 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Schriften des Forschungszentrums Jülich : Reihe Gesundheit 65 Zugl.: Düsseldorf, Univ., Diss., 2012 Lysin (DE-588)4168397-3 gnd rswk-swf Metabolit (DE-588)4138579-2 gnd rswk-swf Corynebacterium glutamicum (DE-588)4309351-6 gnd rswk-swf Biosensor (DE-588)4193016-2 gnd rswk-swf Genomik (DE-588)4776397-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Biosensor (DE-588)4193016-2 s Metabolit (DE-588)4138579-2 s Corynebacterium glutamicum (DE-588)4309351-6 s Lysin (DE-588)4168397-3 s Genomik (DE-588)4776397-8 s DE-604 Schriften des Forschungszentrums Jülich Reihe Gesundheit ; 65 (DE-604)BV035800060 65 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026746532&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Binder, Stephan Rapid development of small-molecule producing microorganisms based on metabolite sensors Schriften des Forschungszentrums Jülich Lysin (DE-588)4168397-3 gnd Metabolit (DE-588)4138579-2 gnd Corynebacterium glutamicum (DE-588)4309351-6 gnd Biosensor (DE-588)4193016-2 gnd Genomik (DE-588)4776397-8 gnd |
| subject_GND | (DE-588)4168397-3 (DE-588)4138579-2 (DE-588)4309351-6 (DE-588)4193016-2 (DE-588)4776397-8 (DE-588)4113937-9 |
| title | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_auth | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_exact_search | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_full | Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder |
| title_fullStr | Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder |
| title_full_unstemmed | Rapid development of small-molecule producing microorganisms based on metabolite sensors Stephan Binder |
| title_short | Rapid development of small-molecule producing microorganisms based on metabolite sensors |
| title_sort | rapid development of small molecule producing microorganisms based on metabolite sensors |
| topic | Lysin (DE-588)4168397-3 gnd Metabolit (DE-588)4138579-2 gnd Corynebacterium glutamicum (DE-588)4309351-6 gnd Biosensor (DE-588)4193016-2 gnd Genomik (DE-588)4776397-8 gnd |
| topic_facet | Lysin Metabolit Corynebacterium glutamicum Biosensor Genomik Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026746532&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035800060 |
| work_keys_str_mv | AT binderstephan rapiddevelopmentofsmallmoleculeproducingmicroorganismsbasedonmetabolitesensors |