Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Volltext http://d-nb.info/1038152062/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-159343 Inhaltsverzeichnis |
| Beschreibung: | 143 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |c Jie Pan. Betreuer |
| 264 | 1 | |c 2013 | |
| 300 | |a 143 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804150747581906944 |
|---|---|
| adam_text | CONTENTS
1 ABSTRACT 7
2 ZUS
AMMENFASSUNG 10
3 INTRODUCTION 13
3.1 PRECLINICAL MOUSE MODEL SYSTEMS FOR THE TRANSLATIONAL RESEARCH OF
HUMAN CANCER 13
3.2 ESTABLISHED CEA424-SV40 T ANTIGEN TRANSGENIC MOUSE MODEL 15
3.3 NEUROENDOCRINE TUMOR MODEL SYSTEM FOR BETTER UNDERSTANDING OF
DISEASE BIOLOGY AND PRECLINICAL
TESTING 18
3.4 EMERGING MOLECULARLY TARGETED THERAPY FOR NEUROENDOCRINE TUMORS 20
3.5 TUMOR INITIATING CELLS OF NEUROENDOCRINE TUMORS IN THE
GASTROINTESTINAL
SYSTEM 22
4 AIM OF THE WORK 27
4.1 IS THE GASTRIC TUMOR
DEVELOPED IN CEA424-SV40 T AG TRANSGENIC MOUSE A NEUROENDOCRINE TUMOR?
27
4.2 CAN THE TUMOR CELL LINES DERIVED FROM THE CEA424-SV40 T AG GASTRIC
TUMORS BE USED FOR SCREENING
NOVEL DRUGS? ARE THESE
SELECTED NOVEL THERAPIES APPLICABLE IN VIVO? HOW IS THE EFFICIENCY AND
SAFETY OF THESE
*TREATMENT? 28
4.3 ARE THERE ANY CANDIDATES FOR THE TUMOR ORIGINATING CELLS OF THE
GASTRIC TUMORS DEVELOPED IN
CEA424-SV40 T AG TRANSGENIC MOUSE? 28
5 MATERIALS AND METHODS 29
5.1 CELL CULTURE 29
5.2 ANIMAL EXPERIMENTS 34
5.3 RNA ISOLATION AND REVERSE TRANSCRIPTION PCR 36
5.4 IMMUNOHISTOCHEMISTRY AND IMMUNOFLUORESCENCE 41
5.5 ELISA 46
4
HTTP://D-NB.INFO/104366954X
5.6 WESTERN BLOT 47
5.7 STATISTICAL ANALYSIS 51
6 RESULTS 52
6.1 PHENOTYPICAL CHARACTERIZATION OF CEA424-SV40
TAG GASTRIC TUMORS 52
6.2 TESTING OF MOLECULARLY TARGETED THERAPIES ON CEA424-SV40 TAG
TRANSGENIC MOUSE MODEL
SYSTEM.
66
6.3 POSSIBLE ESCAPE MECHANISMS OF TUMOR CELLS FROM RADOO1 TREATMENT 83
6.4 RESEARCH ON THE TUMOR ORIGINATING
CELLS 91
7 DISCUSSION 106
7.1 IN DEPTH CHARACTERIZATION OF THE CEA424-SV40 T AG TRANSGENIC MOUSE
SHOWED A CLEAR NEUROENDOCRINE
PHENOTYPE WHICH MADE IT A CLINICALLY RELEVANT MODEL FOR GASTRIC
NEUROENDOCRINE TUMORS 106
7.2 POSSIBLE CONNECTION BETWEEN SV40 TAG TRANSFORMING ABILITY AND THE
NEUROENDOCRINE FEATURES OF THE
MOUSE TUMOR MODEL 108
7.3 TESTING OF MOLECULARLY TARGETED THERAPY ON CEA424-SV40 TAG
TRANSGENIC MOUSE MODEL OFFERED
PROMISING CANDIDATES FOR
THE TREATMENT OF NEUROENDOCRINE TUMORS 110
7.4- POSSIBLE ESCAPE MECHANISM FOR THE TUMOR CELLS UNDER RADOO
1 TREATMENT 112
7.5 POSSIBLE CANDIDATES FOR THE TUMOR
ORIGINATING CELLS IN CEA424-SV40 TAG TRANSGENIC MOUSE 114
8 PERSPECTIVES 118
9 CONCLUSIONS 119
10 EQUIPMENT AND CONSUMABLES 120
10.1 GENERAL MATERIALS 120
10.2 CELL CULTURE 120
10.3 ANIMAL EXPERIMENTS 121
10.4 RNA ISOLATION AND REVERSE TRANSCRIPTION PCR 122
5
10.5 IMMUNOHISTOCHEMISTRY AND IMMUNOFLUORESCENCE 123
10.6 WESTERN BLOT 123
11 ABBREVIATIONS 125
12 REFERENCES 128
13 ACKNOWLEDGEMENTS 142
6
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| language | English |
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| spelling | Pan, Jie Verfasser aut Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors Jie Pan. Betreuer 2013 143 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Ludwig-Maximilians-Universität, Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-159343 http://edoc.ub.uni-muenchen.de/15934/ Verlag kostenfrei Volltext http://d-nb.info/1038152062/34 Langzeitarchivierung Nationalbibliothek https://nbn-resolving.org/urn:nbn:de:bvb:19-159343 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026251382&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pan, Jie Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |
| subject_GND | (DE-588)4113937-9 |
| title | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |
| title_auth | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |
| title_exact_search | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |
| title_full | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors Jie Pan. Betreuer |
| title_fullStr | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors Jie Pan. Betreuer |
| title_full_unstemmed | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors Jie Pan. Betreuer |
| title_short | Molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |
| title_sort | molecularly targeted therapy on a new preclinical mouse model for gastric neuroendocrine tumors |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/15934/ http://d-nb.info/1038152062/34 https://nbn-resolving.org/urn:nbn:de:bvb:19-159343 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026251382&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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