RhoA connects SLP-65 to Pten activation in B-cells:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 114 Bl. Ill., graph. Darst. 30 cm |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENTS
CONTENTS
1 SUMMARY 7
2 INTRODUCTION 9
2.1 IMMUNITY 9
2.2 THE B-LYMPHOCYTE ANTIGEN RECEPTOR COMPLEX 10
2.3 THE B-CELL RECEPTOR DIVERSITY 12
2.4 B-CELL DEVELOPMENT 14
2.4.1 FROM STEM CELLS TO EARLY B-CELL PROGENITORS 15
2.4.2 FROM CLPS TO IMMATURE B CELLS 15
2.4.3 PERIPHERAL DEVELOPMENT OF B CELLS 16
2.5 THE B-CELL SIGNALING PATHWAY 17
2.5.1 THE PI3K PATHWAY 20
2.5.2 THE MAPK/ERK PATHWAY 21
2.5.3 THE SLP-65 DEPENDENT CALCIUM SIGNALING PATHWAY 22
2.6 RHO GTPASES 23
3 OBJECTIVE 25
4 RESULTS 26
4.1 IN-VITRO CHARACTERIZATION OF THE SLP-65-MEDIATED PRE-BCR SIGNALING
COMPLEX 26
4.2 NOVEL SLP-65 INTERACTOME 26
4.2.1 GEF-H1 AS A COMPONENT OF A SLP-65-DEPENDENT PROTEIN COMPLEX 26
4.2.2 AN INDUCIBLE SLP-65 CELL CULTURE SYSTEM TO STUDY THE SLP-65
INTERACTOME 28
4.2.3 TRIPLE SILAC APPROACH IDENTIFIES GEF-H1 AND RHOA AS NOVEL
INTERACTION
PARTNERS OF SLP-65 30
4.3 SLP-65 ACTIVATION LEADS TO RHOA ACTIVATION 36
4.4 SLP-65-REGULATED RHO A ACTIVATION CONTROLS CELLULAR PTEN
LOCALIZATION 37
4.5 RHOA INHIBITION INTERFERES WITH PTEN CLUSTERING IN THE CYTOPLASM 39
4.6 IN-VIVO CHARACTERIZATION OF RHOA FUNCTION IN B CELLS 41
4.7 CONDITIONAL KNOCK-OUT OF RHOA IN EARLY B CELLS IN THE BONE MARROW 41
4.8 INDUCIBLE RHOA GENE DELETION RESULTS IN A DRASTIC DECREASE OF PTEN
EXPRESSION 44
4.9 CONDITIONAL KNOCK-OUT OF RHOA IN PERIPHERAL B CELLS IN THE SPLEEN 45
4.10 RHOA DEFICIENCY LEADS TO IMPAIRED BCR-INDUCED SIGNALING 48
4
HTTP://D-NB.INFO/1037706056
CONTENTS
4.11 RHOA IS REQUIRED FOR B CELL ACTIVATION AND PLASMA CELL
PROLIFERATION 49
4.12 RHOA INDUCES STAT3 ACTIVATION AND PLASMA CELL DIFFERENTIATION 51
4.13 RHOA DEFICIENCY RESULTS IN REDUCED IGG AND IGM SERUM LEVELS 52
4.14 RHOA DEFICIENT MICE SHOW INCREASED AUTOIMMUNE IGGS 53
5 DISCUSSION 57
5.1 SLP-65 INTERACTOME 57
5.2 SLP-65 GEF-HL/RHOA CONNECTION 59
5.3 RHOA IN-VIVO ROLE IN B CELL DEVELOPMENT 60
6 MATERIALS AND METHODS 65
6.1 MATERIALS 65
6.1.1 CHEMICALS AND BIOCHEMICALS 65
6.1.2 KITS 67
6.1.3 BUFFERS AND SOLUTIONS 68
6.1.4 ANTIBODIES, ENZYMES AND PROTEINS 71
6.1.5 CELL LINES 73
6.1.6 BACTERIAL STRAINS 73
6.1.7 RETROVIRAL VECTORS 73
6.1.8 OLIGONUCLEOTIDES 74
6.1.9 LABORATORY EQUIPEMENT 74
6.1.10 SOFTWARE 78
6.1.11 INTERNET TOOLS AND DATABASES 79
6.2 METHODS 80
6.2.1 CELL CULTURE 80
6.2.2 SILAC LABELING 80
6.2.3 FREEZING AND THAWING OF CELLS 80
6.2.4 IMMUNOFLUORESCENCE MICROSCOPY 81
6.2.5 MOLECULAR CLONING 83
6.2.6 CELLULAR BIOLOGY 88
6.2.7 MICE WORK 90
6.2.8 PROTEIN BIOCHEMISTRY 92
6.2.9 MASS SPECTROMETRY 96
7 SUPPLEMENTARY 100
8 REFERENCES 101
5
CONTENTS
9 APPENDIX 1 110
10 APPENDIX 2 (ABBREVIATIONS) 111
11 ACKNOWLEDGMENTS 114
6
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| any_adam_object | 1 |
| author | Outaleb, Issame |
| author_GND | (DE-588)1036378349 |
| author_facet | Outaleb, Issame |
| author_role | aut |
| author_sort | Outaleb, Issame |
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| bvnumber | BV041253876 |
| ctrlnum | (OCoLC)859390875 (DE-599)DNB1037706056 |
| dewey-full | 571.967 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.967 |
| dewey-search | 571.967 |
| dewey-sort | 3571.967 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:43:15Z |
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| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026227866 |
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| spelling | Outaleb, Issame Verfasser (DE-588)1036378349 aut RhoA connects SLP-65 to Pten activation in B-cells vorgelegt von Issame Outaleb 2012 114 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026227866&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Outaleb, Issame RhoA connects SLP-65 to Pten activation in B-cells |
| subject_GND | (DE-588)4113937-9 |
| title | RhoA connects SLP-65 to Pten activation in B-cells |
| title_auth | RhoA connects SLP-65 to Pten activation in B-cells |
| title_exact_search | RhoA connects SLP-65 to Pten activation in B-cells |
| title_full | RhoA connects SLP-65 to Pten activation in B-cells vorgelegt von Issame Outaleb |
| title_fullStr | RhoA connects SLP-65 to Pten activation in B-cells vorgelegt von Issame Outaleb |
| title_full_unstemmed | RhoA connects SLP-65 to Pten activation in B-cells vorgelegt von Issame Outaleb |
| title_short | RhoA connects SLP-65 to Pten activation in B-cells |
| title_sort | rhoa connects slp 65 to pten activation in b cells |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026227866&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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