Effect of neuronal autoantibodies on enteric neurons and vagal afferents:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130801-1142264-0-1 Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | 121 Bl. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Qin, Li |d 1960- |e Verfasser |0 (DE-588)1041250185 |4 aut | |
| 245 | 1 | 0 | |a Effect of neuronal autoantibodies on enteric neurons and vagal afferents |c Qin Li |
| 264 | 1 | |c 2013 | |
| 300 | |a 121 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. Sprache | ||
| 502 | |a München, Techn. Univ., Diss., 2013 | ||
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Datensatz im Suchindex
| _version_ | 1804150703705292800 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
PUBLICATIONS 1
PUBLISHED ABSTRACT 2
ABBREVIATIONS 3
DECLARATION 6
ZUSAMMENFASSUNG 10
ABSTRACT 12
1. INTRODUCTION 14
1.1 GENERAL INTRODUCTION 14
1.2 INNERVATION OF THE GUT 15
1.2.1 EXTRINSIC AFFERENT INNERVATION 15
1.2.2 VAGAL AFFERENT FIBRES 16
1.2.3 THE VAGAL AFFERENT TERMINAL: STRUCTURE AND DISTRIBUTION OF IGLES
AND IMAS 17
1.2.4 ELECTROPHYSIOLOGICAL CHARACTERISTICS OF VAGAL AFFERENT TERMINALS
19
1.2.5 PHYSIOLOGICAL FUNCTION OF VAGAL AFFERENT TERMINALS 20
1.3 ENTERIC NERVOUS SYSTEM 21
1.3.1 STRUCTURE OF THE ENS 21
1.3.2 CLASSIFICATION OF NEURONS IN THE ENS 23
1.3.3 TYPES OF NEURONAL TRANSMISSION IN ENS 26
1.3.4 IMMUNO-NEURAL INTERACTIONS 27
1.4 PARANEOPLASTIC SYNDROMES 28
1.4.1 TYPES OF PARANEOPLASTIC SYNDROMES 28
1.4.2 HISTORICAL CONSIDERATIONS 29
1.4.3 PARANEOPLASTIC GASTROINTESTINAL DYSMOTILITY 29
1.5 ANTINEURONAL AUTOANTIBODIES 32
1.5.1 ANNA-1 (ANTI-HU) 33
1.5.2 HU ANTIGEN 33
1.5.3 ANTIBODIES AGAINST ACETYLCHOLINE RECEPTORS 34
2. MATERIALS AND METHODS 36
2. 1 PATIENT MATERIAL 36
2.1.1 SERUM IGG SAMPLES 36
2.1.2 ANTI-HUD AND ANTI-HUA/B/C FRACTIONS WERE ISOLATED BY AFFINITY
CHROMATOGRAPHY 39
2.2 IMMUNOHISTOCHEMISTRY 40
7
HTTP://D-NB.INFO/1041250223
IMAGE 2
2.3 NEUROIMAGING STUDIES 40
2.3.1 ANIMALS 40
2.3.2 HUMAN TISSUE SPECIMEN 41
2.3.3 TISSUE PREPARATION 41
2.3.4 VOLTAGE SENSITIVE DYE LABELING 42
2.3.5 NEUROIMAGING WITH MULTISITE OPTICAL RECORDING TECHNIQUE 42
2.3.6 SOLUTIONS AND DRUG APPLICATION 44
2.3.7 DATA ANALYSIS AND STATISTICS 45
2.4 EXTRACELLULAR RECORDINGS FROM VAGAL AFFERENTS 46
2.4.1 ANIMALS 46
2.4.2 TISSUE PREPARATION 46
2.4.3 AFFERENT RECORDINGS 47
2.4.4 MECHANOSENSITIVE RECEPTIVE FIELD (HOT SPOTS) IDENTIFICATION 48
2.4.5 SOLUTIONS AND DRUG APPLICATION 48
2.4.6 DATA ANALYSIS AND STATISTICS 49
3. RESULTS 50
3.1 IMMUNOHISTOCHEMISTRY 50
3.1.1 THE IMMUNOSTAINING OF THE SERUM IGG SAMPLES IN THE GUINEA PIG 50
3.1.2 THE IMMUNOSTAINING OF THE ANTI-HUD ANTIBODY AND THE ANTI-HUA/B/C
ANTIBODIES IN
THE GUINEA PIG 52
3.2 NEUROIMAGING STUDIES 52
3.2.1 A3-ACHR AND ANNA-1 IGG SAMPLES EVOKE ENTERIC NEURON EXCITATION IN
GUINEA PIG
AND IN HUMAN 53
3.2.1.1 GUINEA PIG MYENTERIC NEURONS RESPONDED TO SERUM IGG 53
3.2.1.2 HUMAN SUBMUCOUS NEURONS RESPONDED TO IGG SERUM 58
3.2.2 WHICH ANTIBODY IS RESPONSIBLE FOR THE EXCITATORY EFFECT ON
MYENTERIC NEURONS?.... 63
3.2.2.1 RESPONSE TO ANTI-HUD AND COMPARISON WITH ANTI-HUA/B/C IN THE
GUINEA PIG
MYENTERIC NEURONS 64
3.2.2.2 RESPONSES TO ANTI-HUD IN COMPARISON WITH BLOOD DONOR SERUM IN
GUINEA PIG
MYENTERIC NEURONS 65
3.2.2.3 RESPONSES TO ANTI-HUD COMPARED WITH HUD ANTIGEN IN THE GUINEA
PIG
MYENTERIC NEURONS 66
8
IMAGE 3
3.2.2.4 RESPONSES TO PATIENT SERUM WAS SIMILAR TO RESPONSES TO ANTI-HUD
ANTIBODY IN
THE GUINEA PIG MYENTERIC NEURONS 72
3.2.2.5 RESPONSES TO PATIENT SERUM COMPARED TO BLOOD DONOR SERUM AND HC
72
3.2.3 THE NICOTINIC ACETYLCHOLINE RECEPTOR BLOCKER HEXAMETHONIUM REDUCES
ANNA-1
INDUCED NEURONAL RESEPONSES IN GUINEA PIG MYENTERIC NEURONS 75
3.2.4 ESTIMATION OF THE CONCENTRATION OF THE ANTIBODIES AT THE LEVEL OF
THE GANGLION 77
3.2.5 POSSIBLE INHIBITORY EFFECTS OF ANTIBODIES AND REPRODUCIBILITY OF
THE ANTIBODY INDUCED
EFFECT 77
3.3 EXTRACELLULAR RECORDINGS FROM VAGAL AFFERENTS 82
3.3.1 EFFECT OF ATP AND DMPP ON VAGAL AFFERENTS OF THE MURINE GASTRIC
FUNDUS 82
3.3.2 EFFECT OF ANTI-HUD AND ANTI-HUA/B/C ON VAGAL AFFERENTS SUPPLYING
THE GASTRIC
FUNDUS 85
3.3.3 EFFECT OF ANTI-HUD ON GASTRIC VAGAL AFFERENTS IN CALCIUM-FREE
KREBS BUFFER 85
4. DISCUSSION 91
REFERENCES 99
LIST OF TABLES 116
LIST OF FIGURES 117
ACKNOWLEDGMENTS 119
CURRICULUM VITAE (ENGLISH) 120
CURRICULUM VITAE (GERMAN) 121
9
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:43:09Z |
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| language | English |
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| physical | 121 Bl. Ill., graph. Darst. |
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| publishDateSort | 2013 |
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| spelling | Qin, Li 1960- Verfasser (DE-588)1041250185 aut Effect of neuronal autoantibodies on enteric neurons and vagal afferents Qin Li 2013 121 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache München, Techn. Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20130801-1142264-0-1 http://mediatum.ub.tum.de/node?id=1142264 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130801-1142264-0-1 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026223491&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Qin, Li 1960- Effect of neuronal autoantibodies on enteric neurons and vagal afferents |
| subject_GND | (DE-588)4113937-9 |
| title | Effect of neuronal autoantibodies on enteric neurons and vagal afferents |
| title_auth | Effect of neuronal autoantibodies on enteric neurons and vagal afferents |
| title_exact_search | Effect of neuronal autoantibodies on enteric neurons and vagal afferents |
| title_full | Effect of neuronal autoantibodies on enteric neurons and vagal afferents Qin Li |
| title_fullStr | Effect of neuronal autoantibodies on enteric neurons and vagal afferents Qin Li |
| title_full_unstemmed | Effect of neuronal autoantibodies on enteric neurons and vagal afferents Qin Li |
| title_short | Effect of neuronal autoantibodies on enteric neurons and vagal afferents |
| title_sort | effect of neuronal autoantibodies on enteric neurons and vagal afferents |
| topic_facet | Hochschulschrift |
| url | http://mediatum.ub.tum.de/node?id=1142264 https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130801-1142264-0-1 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026223491&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT qinli effectofneuronalautoantibodiesonentericneuronsandvagalafferents |