Protein analysis with the switchSENSE biosensor:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Garching
Verein zur Förderung des Walter-Schottky-Inst. der Techn. Univ. München
2013
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Selected topics of semiconductor physics and technology
163 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | II, 148 S. Ill., graph. Darst. 21 cm |
| ISBN: | 9783941650633 |
Internformat
MARC
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| 020 | |a 9783941650633 |c kart. |9 978-3-941650-63-3 | ||
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| 049 | |a DE-12 |a DE-91 | ||
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| 084 | |a PHY 855d |2 stub | ||
| 084 | |a CHE 828d |2 stub | ||
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| 100 | 1 | |a Knežević, Jelena |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Protein analysis with the switchSENSE biosensor |c Jelena Knežević |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Garching |b Verein zur Förderung des Walter-Schottky-Inst. der Techn. Univ. München |c 2013 | |
| 300 | |a II, 148 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Selected topics of semiconductor physics and technology |v 163 | |
| 502 | |a Zugl.: München, Techn. Univ., Diss., 2013 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 830 | 0 | |a Selected topics of semiconductor physics and technology |v 163 |w (DE-604)BV011499438 |9 163 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-026196568 | ||
Datensatz im Suchindex
| _version_ | 1804150659560243200 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
1 INTRODUCTION 1
2 FUNDAMENTALS 3
2.1 DNA 3
2.2 PROTEINS 6
2.3 FLUORESCENCE QUENCHING IN THE PROXIMITY OF A METAL SURFACE 9
2.4 METAL-SOLUTION INTERFACE 15
2.5 ELECTRICALLY SWITCHABLE DNA LAYERS 19
3 EXPERIMENTAL SETUP 24
4 QUANTIFICATION OF AFFINITY, AVIDITY, AND BINDING KINETICS OF PROTEIN
ANALYTES
BY THE TIME-RESOLVED MOLECULAR DYNAMICS 31
4.1 ESTIMATION OF THE AFFINITY AND AVIDITY OF HIS 6-PROTEINS BOUND TO
TRISNTA-DNA LAYERS 36
4.2 REAL-TIME DETECTION OF HIS 6-PROTEIN A BINDING TO TRISNTA-DNA LAYER
39
4.3 INFLUENCE OF THE HIS 6-TAG ACCESSIBILITY ON THE
HISE-PROTEIN/TRISNTA-DNA BINDING KINETICS 46
4.4 CONCLUSION 50
5 PROTEIN SIZE DETERMINATION BY THE TIME-RESOLVED PROTEIN-DNA SWITCHING
DYNAMICS 51
5.1 CONCLUSION 65
6 DETERMINATION OF THE ISOELECTRIC POINT OF PROTEIN BY THE ELECTRICALLY
INDUCED
SWITCHING OF PROTEIN-DNA LAYERS 66
6.1 SIZE AND ZETA POTENTIAL DETERMINATION OF AVIDIN AND STREPTAVIDIN
WITH LIGHT SCATTERING METHODS 67
6.2 DETERMINATION OF THE ISOELECTRIC POINT OF AVIDIN FROM THE PH
DEPENDENT SWITCHING DYNAMICS OF AVIDIN-DNA LAYER 75
6.3 DISSIMILAR SWITCHING DYNAMICS OF STREPTAVIDIN-DNA AND AVIDIN-DNA
LAYERS AT PH 8.1 ! 85
6.4 CONCLUSION 93
HTTP://D-NB.INFO/103792648X
IMAGE 2
7 DETECTION OF DNA CONFORMATIONAL CHANGES UPON BINDING OF A
TRANSCRIPTION
PROTEIN 94
7.1 DETECTION OF THE LOCUS OF PROTEIN BINDING SITE WITHIN DNA SEQUENCE
98
7.2 DETERMINATION OF THE IHF-H DNA BINDING AFFINITY 102
7.3 DETERMINATION OF THE IHF-H DNA ASSOCIATION RATE 104
7.4 CONCLUSION 109
8 CONCLUSION AND OUTLOOK 110
APPENDIX 113
LIST OF PUBLICATIONS AND PATENTS 133
ACKNOWLEDGMENTS 135
REFERENCES 136
|
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| classification_tum | PHY 855d CHE 828d |
| ctrlnum | (OCoLC)856873449 (DE-599)DNB103792648X |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.636 |
| dewey-search | 572.636 |
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| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Physik Biologie Chemie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:42:26Z |
| institution | BVB |
| isbn | 9783941650633 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026196568 |
| oclc_num | 856873449 |
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| physical | II, 148 S. Ill., graph. Darst. 21 cm |
| publishDate | 2013 |
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| series | Selected topics of semiconductor physics and technology |
| series2 | Selected topics of semiconductor physics and technology |
| spelling | Knežević, Jelena Verfasser aut Protein analysis with the switchSENSE biosensor Jelena Knežević 1. Aufl. Garching Verein zur Förderung des Walter-Schottky-Inst. der Techn. Univ. München 2013 II, 148 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Selected topics of semiconductor physics and technology 163 Zugl.: München, Techn. Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Selected topics of semiconductor physics and technology 163 (DE-604)BV011499438 163 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026196568&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Knežević, Jelena Protein analysis with the switchSENSE biosensor Selected topics of semiconductor physics and technology |
| subject_GND | (DE-588)4113937-9 |
| title | Protein analysis with the switchSENSE biosensor |
| title_auth | Protein analysis with the switchSENSE biosensor |
| title_exact_search | Protein analysis with the switchSENSE biosensor |
| title_full | Protein analysis with the switchSENSE biosensor Jelena Knežević |
| title_fullStr | Protein analysis with the switchSENSE biosensor Jelena Knežević |
| title_full_unstemmed | Protein analysis with the switchSENSE biosensor Jelena Knežević |
| title_short | Protein analysis with the switchSENSE biosensor |
| title_sort | protein analysis with the switchsense biosensor |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026196568&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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