The role of the inhibitory receptor Siglec-G in the development of autoimmunity: = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | German English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 117 S. Ill., graph. Darst. |
Internformat
MARC
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| 245 | 1 | 0 | |a The role of the inhibitory receptor Siglec-G in the development of autoimmunity |b = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |c vorgelegt von Susanne Bökers |
| 246 | 1 | 1 | |a Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |
| 264 | 1 | |c 2013 | |
| 300 | |a 117 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2013 | ||
| 546 | |a Mit einer Zssfassung in dt. Sprache | ||
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| 650 | 0 | 7 | |a Inhibitorpeptide |0 (DE-588)4319976-8 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804150654197825536 |
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| adam_text | IMAGE 1
1. INTRODUCTION 1
1.1 DEVELOPMENT AND FUNCTION OF B LYMPHOCYTES 1
1.2 IMMUNOLOGICAL TOLERANCE 3
1.3 BREAKDOWN OF SELF-TOLERANCE 4
1.4 SIGLEC-FAMILY 7
1.5 SIGLEC-G AND ITS HUMAN ORTHOLOGUE SIGLEC-10 10
1.6 FUNCTION OF SIGLEC-G IDENTIFIED VIA DEFICIENT MOUSE-LINES 10
1.7 SIGLECS AND THE MAINTENANCE OF IMMUNOLOGICAL TOLERANCE 12
1.8 RHEUMATOID ARTHRITIS AND COLLAGEN-INDUCED ARTHRITIS 13
1.9 SYSTEMIC LUPUS ERYTHEMATOSUS 16
1.10 THE MRL/MPJ FAS/LPR STRAIN AS CLASSICAL MOUSE MODEL FOR SYSTEMIC
LUPUS
ERYTHEMATOSUS 17
1.11 ROLE OF LYMPHOCYTES IN SLE 18
1.12 B1 CELLS IN SLE 20
1.13 AIM OF THE SUBJECT 22
2. MATERIAL 23
2.1. ANTIBODIES 23
2.1.1 ANTIBODIES FOR ELISA 23
2.1.2 ANTIBODIES AND STREPTAVIDIN-CONJUGATES FOR FLOW CYTOMETRY 23
2.1.3 ANTIBODIES AND STREPTAVIDIN CONJUGATES FOR IMMUNOHISTOCHEMISTRY 25
2.2 REAGENTS, CHEMICALS AND CONSUMABLES ; 25
2.3 MOUSE STRAINS 26
2.4 BUFFER, FLUIDS AND SOLUTIONS 27
2.5 OLIGONUCLEOTIDES 29
2.5.1 PCR-PRIMER FOR BACKCROSSING 29
2.5.2 PCR-PRIMER FOR GENOTYPING 30
2.6 KITS 31
2.7 EQUIPMENT 31
2.8 SOFTWARE 31
HTTP://D-NB.INFO/1038655285
IMAGE 2
3. METHODS 32
3.1 ANIMAL HUSBANDRY 32
3.2 BACKCROSSING THE SIGLEC-G DEFICIENCY INTO THE MRL/MPJ FAS/LPR STRAIN
32
3.3 COLLAGEN INDUCED ARTHRITIS 33
3.3.1 PREPARATION OF CHICKEN TYPE II COLLAGEN EMULSION 33
3.3.2 INJECTION OF CHICKEN TYPE II COLLAGEN EMULSION 33
3.3.3 CLINICAL SCORING OF CIA SYMPTOMS 33
3.3.4 SCORING OF HISTOLOGICAL CIA PARAMETERS 33
3.4 ELISA 34
3.4.1 SERUM TITER ELISA: 34
3.4.2 ANTI-DSDNA ELISA 34
3.4.3 RHEUMATOID FACTOR ELISA 35
3.4.4 SM/RNP-ELISA 35
3.4.5 COLLAGEN-ELISA 35
3.5 HARVEST AND SINGLE CELL PREPARATION OF DIFFERENT ORGANS 36
3.5.1 HARVEST OF ORGANS WITH SUBSEQUENT FORMALIN FIXATION 36
3.5.2 HARVEST OF ORGANS WITH SUBSEQUENT SINGLE CELL PREPARATION 36
3.5.3 PREPARATION OF CELLS FROM THE PERITONEAL CAVITY 36
3.6 HISTOLOGY 37
3.6.1 DECALCIFICATION OF KNEES 37
3.6.2 DEHYDRATION, PARAFFINATION AND SECTIONING OF ORGANS 37
3.6.3 H+E STAINING 37
3.6.4 PERIODIC ACID SCHIFF(PAS) STAINING 38
3.6.5 ANALYSIS OF HISTOLOGICAL SECTIONS 38
3.7 IMMUNOHISTOCHEMISTRY: 38
3.8 FLOW CYTOMETRY 39
3.8.1 LIVE CELL COUNT 39
3.8.2 STAINING 39
IMAGE 3
3.8.3 ANALYSIS OF FLOW CYTOMETRY 40
3.9 MEASUREMENT OF PROTEINURIA 40
3.10 DETECTION OF ANTI-NUCLEAR ANTIBODIES (ANA) 40
3.11 POLYMERASE CHAIN REACTION (PCR) 41
3.11.1 ISOLATION OF MOUSE DNA FROM EAR OR TAIL BIOPSY 41
3.11.2 PCR FOR GENOTYPING: 41
3.11.3 PCR TO DETECT GENETIC MARKERS (MICROSATELLITES) IN BALB/C AND MRL
MICE....42
3.11.4 AGAROSE GEL ELECTROPHORESIS 42
3.12 BLOOD UREA NITROGEN (BUN) 42
3.13 STATISTICAL ANALYSIS: 43
4 RESULTS 44
4.1 COLLAGEN INDUCED ARTHRITIS (CIA) 44
4.1.1 CLINICAL ARTHRITIS SCORES ON PAWS ARE HIGHER IN SIGLEC-G DEFICIENT
MICE 44
4.1.2 CUMULATIVE INCIDENCE OF COLLAGEN INDUCED ARTHRITIS 45
4.1.3 BLINDED SCORING OF H+E STAINED JOINTS SHOWED HIGHER SEVERITY OF
ARTHRITIS OUTCOME IN SIGLEC-G DEFICIENT MICE 46
4.1.4 ANALYSIS OF CELL POPULATIONS AFTER INDUCTION OF COLLAGEN INDUCED
ARTHRITIS 48
4.1.5 IGM LEVELS AGAINST CHICKEN TYPE II COLLAGEN ARE INCREASED IN
SIGLEC-G DEFICIENT CIA MICE 49
4.2 BACKCROSSING THE SIGLEC-G-DEFICIENCY INTO THE MRL/MPJ FAS/IPR STRAIN
VIA THE GENERATION OF SPEED CONGENICS 51
4.2.1 GENOTYPIC CHARACTERISATION 51
4.3 PHENOTYPIC CHARACTERISATION OF SIGLEC-G DEFICIENT MRL/LPR MICE 52
4.3.1 DETECTION OF IG SERUM TITRE VIA ELISA REVEALED DIFFERENCES BETWEEN
SIGLEC-G DEFICIENT AND WT MRL/LPR MICE 52
4.3.2 DETECTION OF AUTOREACTIVE ANTIBODIES 54
4.3.3 SIMILAR SPLEEN WEIGHT OF SIGLEC-G AND WT MRL/LPR MICE 62
4.3.4 FLOW CYTOMETRY ANALYSIS OF CELL POPULATIONS OF MRL/LPR MICE 63
4.3.5 ANALYSIS OF IMMUNE COMPLEX DEPOSITION IN THE KIDNEYS 72
4.3.6 HISTOLOGY ON KIDNEYS DID NOT REVEAL DIFFERENCES IN PATHOLOGICAL
ALTERATIONS 74
IMAGE 4
4.3.7 CUMULATIVE PROTEINURIA SCORE 75
4.3.8 PROTEINURIA 300 MG/DL OCCURS EARLIER IN FEMALE SIGLEC-G
DEFICIENT THAN IN WT MICE 76
4.3.9 BLOOD UREA NITROGEN LEVELS ARE SIMILAR BETWEEN SIGLEC-G AND WT
MRL/LPR MICE 77
4.3.10 SPONTANEOUS GERMINAL CENTRE (GC) FORMATION 78
4.3.11 NO DIFFERENCE IN THE INCIDENCE OF ARTHRITIS 80
4.3.12 OVERALL LIFESPAN IS SIMILAR IN SIGLEC-G AND WT MRL/LPR MICE 81
5. DISCUSSION 83
5.1 SUCCESSFUL INDUCTION OF CIA IN RESISTANT STRAIN BALB/C 83
5.2 THE LOSS OF SIGLEC-G EXACERBATES COLLAGEN INDUCED ARTHRITIS 83
5.3 POSSIBLE ROLE FOR SIGLEC-G IN AMELIORATING CIA VIA INHIBITION OF TLR
SIGNALLING 86
5.4 SIGLEC-G AND WT MRL/LPR MICE DEVELOP LUPUS LIKE DISEASE 87
5.5 THE LOSS OF SIGLEC-G LEADS TO AN EARLIER ONSET OF LUPUS-LIKE DISEASE
87
5.6 NO ENHANCED SEVERITY OF KIDNEY DAMAGE IN SIGLEC-G DEFICIENT MRL/LPR
MICE 89
5.7 ELEVATED CELL NUMBERS AND ACTIVATION STATE OF THE IMMUNE SYSTEM IN
SIGLEC-G DEFICIENT MRL/LPR 90
5.8 LOSS OF SIGLEC-G LEADS TO A WORSENING OF LUPUS LIKE SYMPTOMS IN
MRL/LPR MICE BUT
NOT TO ALTERATIONS IN OVERALL SURVIVAL 92
6. SUMMARY 94
7. ZUSAMMENFASSUNG 95
8. REFERENCES 97
9. OWN PUBLICATIONS 112
10. APPENDIX 113
10.1 ABBREVIATIONS 113
10.2. ACKNOWLEDGMENTS 117
|
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| author | Bökers, Susanne |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:42:21Z |
| institution | BVB |
| language | German English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-026193004 |
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| owner_facet | DE-29 DE-29T DE-188 DE-12 DE-355 DE-BY-UBR |
| physical | 117 S. Ill., graph. Darst. |
| publishDate | 2013 |
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| spelling | Bökers, Susanne Verfasser (DE-588)1038655064 aut The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität vorgelegt von Susanne Bökers Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität 2013 117 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2013 Mit einer Zssfassung in dt. Sprache Autoimmunität (DE-588)4319435-7 gnd rswk-swf Inhibitorpeptide (DE-588)4319976-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Autoimmunität (DE-588)4319435-7 s Inhibitorpeptide (DE-588)4319976-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026193004&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bökers, Susanne The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität Autoimmunität (DE-588)4319435-7 gnd Inhibitorpeptide (DE-588)4319976-8 gnd |
| subject_GND | (DE-588)4319435-7 (DE-588)4319976-8 (DE-588)4113937-9 |
| title | The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |
| title_alt | Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |
| title_auth | The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |
| title_exact_search | The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |
| title_full | The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität vorgelegt von Susanne Bökers |
| title_fullStr | The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität vorgelegt von Susanne Bökers |
| title_full_unstemmed | The role of the inhibitory receptor Siglec-G in the development of autoimmunity = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität vorgelegt von Susanne Bökers |
| title_short | The role of the inhibitory receptor Siglec-G in the development of autoimmunity |
| title_sort | the role of the inhibitory receptor siglec g in the development of autoimmunity die rolle des inhibitorischen rezeptors siglec g bei der entwicklung von autoimmunitat |
| title_sub | = Die Rolle des inhibitorischen Rezeptors Siglec-G bei der Entwicklung von Autoimmunität |
| topic | Autoimmunität (DE-588)4319435-7 gnd Inhibitorpeptide (DE-588)4319976-8 gnd |
| topic_facet | Autoimmunität Inhibitorpeptide Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026193004&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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