The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE):
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 108 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) |c vorgelegt von Xueli Zhang |
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
ABSTRACT I
LIST O F FIGURES V
LIST O F TABLES VII
ABBREVIATIONS VIII
1 INTRODUCTION 1
1.1 AUTOIMMUNE DISEASE 1
1.1.1 MULTIPLE SCLEROSIS (MS) 1
1.1.2 EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE) 2
1.1.3 THE ROUTES O F IMMUNE CELL ENTRY INTO THE CNS IN EAE 5
1.1.4 IMMUNE CELLS INVOLVED IN EAE 10
1.1.4.1 T LYMPHOCYTES 10
1.1.4.2 MACROPHAGES 12
1.1.4.3 B LYMPHOCYTES AND ANTIBODIES 13
1.1.5 CHEMOKINES AND CHEMOKINE RECEPTORS 15
1.2 SYNDECAN FAMILY AND SYNDECAN-1 (CD 138) 18
1.2.1 THE STRUCTURE O F SYNDECANS 19
1.2.2 THE EXPRESSION O F SYNDECANS 20
1.2.3 THE FUNCTION O F SYNDECANS 21
1.2.4 SYNDECAN-1 (CD 138) AND INFLAMMATION 24
1.2.4.1 SYNDECAN-1 ON ENDOTHELIAL CELLS OR EPITHELIAL CELLS CAN INHIBIT
ADHESION
MOLECULE ACTIVITY 26
1.2.4.2 SYNDECAN-1 MODULATES CYTOKINE CONCENTRATIONS AND CHEMOKINE
GRADIENTS DURING INFLAMMATION 27
1.3 THE AIM O F THIS STUDY 29
2 MATERIALS AND METHODS 31
2.1 MATERIALS 31
2.1.1 MICE 31
II
HTTP://D-NB.INFO/1037858239
IMAGE 2
2.1.2 CHEMICALS 31
2.1.3 ANTIBODIES 34
2.1.4 GENERAL BUFFERS AND SOLUTIONS 37
2.1.5 CELL CULTURE MEDIUM AND SUPPLEMENTS 38
2.1.6 TECHNICAL EQUIPMENT 38
2.1.7 SOFTWARE 39
2.2 METHODS 40
2.2.1 ANIMAL MODEL O F EAE 40
2.2.1.1 ACTIVE EAE INDUCTION 40
2.2.1.2 PASSIVE EAE INDUCTION 41
2.2.1.3 BONE MARROW CHIMERIC MICE 42
2.2.2 FLOW CYTOMETRY 42
2.2.2.1 ISOLATION O F MONONUCLEAR CELLS FROM THE CNS AND PERIPHERY 42
2.2.2.1.1 ISOLATION O F MONONUCLEAR CELLS FROM THE CNS 42
2.2.2.1.2 ISOLATION O F MONONUCLEAR CELLS FROM BLOOD 43
2.2.2.1.3 ISOLATION O F MONONUCLEAR CELLS FROM LYMPH NODES AND SPLEENS
....43
2.2.2.2 STAINING FOR FLOW CYTOMETRY 44
2.2.2.2.1 CELL SURFACE STAINING 44
2.2.2.2.2 INTRACELLULAR STAINING 44
2.2.3 MORPHOLOGICAL ANALYSIS 45
2.2.3.1 SAMPLE COLLECTION 45
2.2.3.2 IMMUNOFLUORESCENCE STAINING 46
2.2.4 T CELL PROLIFERATION ASSAYS 46
2.2.4.1 IN VITRO PROLIFERATION ASSAY 46
2.2.4.2 IN VIVO PROLIFERATION ASSAY 47
2.2.5 CHOROID PLEXUS ISOLATION AND CEREBROSPINAL FLUID EXTRACTION 47
2.2.6 DOT BLOTS 48
2.2.7 CHEMOKINE AND CYTOKINE MEASUREMENT 49
2.2.8 REVERSE TRANSCRIPTION AND QUANTITATIVE PCR (Q-PCR) 49
2.2.9 MEASUREMENT O F PLASMA CELL NUMBERS AND TOTAL OR MOG35_55-SPECIFIC
SERUM
III
IMAGE 3
TITERS 51
2.2.10 ELISA FOR IL-6/SYNDECAN-L OR CCL20/SYNDECAN-1 BINDING 52
2.2.11 STATISTICAL ANALYSIS 52
3. RESULTS 53
3.1 SDC-1 1 MICE SHOW INCREASED EAE SEVERITY AND IMPAIRED RECOVERY 53
3.2 IN VIVO EXPRESSION O F SYNDECAN-1 B Y CNS RESIDENT AND IMMUNE CELLS
61
3.3 ROLE O F SYNDECAN-1 ON W T CHOROID PLEXUS EPITHELIUM 66
3.4 LOWER LEVELS O F CCL20 ON CHOROID PLEXUS EPITHELIUM IN SDC-1 1
MICE AND
HIGHLY ELEVATED IL-6 LEVELS 70
3.5 IMMUNE CELL SOURCE O F SYNDECAN-1 74
4. DISCUSSION 77
4.1 SYNDECAN-1 EXPRESSION IN THE BRAIN 77
4.2 THE RELATIONSHIP AMONG SYNDECAN-1, CCL20 AND IL-6 78
4.3 CHEMOKINE LEVELS ARE INCREASED IN THE CNS AND CSF DURING EAE 81
4.4 SYNDECAN-1 AND ADHESION COMPLEXES 82
4.5 PLASMA CELLS ARE INVOLVED IN THE RECOVERY PHASE O F EAE 83
4.6 CONCLUSION 84
5. FUTURE PERSPECTIVES 85
6. REFERENCES 88
PUBLICATIONS 104
CURRICULUM VITAE 105
ACKNOWLEDGEMENTS 106
IV
|
| any_adam_object | 1 |
| author | Zhang, Xueli 1984- |
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| author_facet | Zhang, Xueli 1984- |
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| bvnumber | BV041211380 |
| ctrlnum | (OCoLC)858928237 (DE-599)BSZ391734474 |
| dewey-full | 610 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 610 - Medicine and health |
| dewey-raw | 610 |
| dewey-search | 610 |
| dewey-sort | 3610 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:42:11Z |
| institution | BVB |
| language | English |
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| physical | XII, 108 S. Ill., graph. Darst. |
| publishDate | 2013 |
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| spelling | Zhang, Xueli 1984- Verfasser (DE-588)103732689X aut The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) vorgelegt von Xueli Zhang 2013 XII, 108 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Münster (Westfalen), Univ., Diss., 2013 Proteoglykane (DE-588)4176001-3 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Heparansulfat (DE-588)4242601-7 gnd rswk-swf Tiermodell (DE-588)4140660-6 gnd rswk-swf Encephalomyelitis (DE-588)4152136-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Maus (DE-588)4169148-9 s Tiermodell (DE-588)4140660-6 s Encephalomyelitis (DE-588)4152136-5 s Heparansulfat (DE-588)4242601-7 s Proteoglykane (DE-588)4176001-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026186078&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zhang, Xueli 1984- The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) Proteoglykane (DE-588)4176001-3 gnd Maus (DE-588)4169148-9 gnd Heparansulfat (DE-588)4242601-7 gnd Tiermodell (DE-588)4140660-6 gnd Encephalomyelitis (DE-588)4152136-5 gnd |
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| title | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) |
| title_auth | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) |
| title_exact_search | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) |
| title_full | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) vorgelegt von Xueli Zhang |
| title_fullStr | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) vorgelegt von Xueli Zhang |
| title_full_unstemmed | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) vorgelegt von Xueli Zhang |
| title_short | The role of the transmembrane heparan sulfate proteoglycan, Syndecan-1 (CD138), in murine experimental autoimmune encephalomyelitis (EAE) |
| title_sort | the role of the transmembrane heparan sulfate proteoglycan syndecan 1 cd138 in murine experimental autoimmune encephalomyelitis eae |
| topic | Proteoglykane (DE-588)4176001-3 gnd Maus (DE-588)4169148-9 gnd Heparansulfat (DE-588)4242601-7 gnd Tiermodell (DE-588)4140660-6 gnd Encephalomyelitis (DE-588)4152136-5 gnd |
| topic_facet | Proteoglykane Maus Heparansulfat Tiermodell Encephalomyelitis Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026186078&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zhangxueli theroleofthetransmembraneheparansulfateproteoglycansyndecan1cd138inmurineexperimentalautoimmuneencephalomyelitiseae |