Glycosyltransferases: methods and protocols
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2013
|
| Schriftenreihe: | Methods in molecular biology
1022 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 425 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
| _version_ | 1804150523482341376 |
|---|---|
| adam_text | Titel: Glycosyltransferases
Autor: Brockhausen, Inka
Jahr: 2013
Contents
Preface.......................................................... v
Contributors.......................................................... xiii
1 Glycan Microarray Screening Assay for Glycosyltransferase Specificities...... 1
Wenjie Peng, Corwin M. Nycholat, and Nahid Razi
2 A Fluorescence-Based Assay for Core 1 ß3Galactosyltransferase
(T-Synthase) Activity............................................ 15
Tongzbong Ju and Richard D. Cumminjjs
3 Structural and Biochemical Analysis of a Bacterial Glycosyltransferase....... 29
Pan 23m, Ren Wu, Hua Zhang, and Hut Wu
4 Study of the Biological Functions of Mucin Type Core 3 O-glycans........ 41
Seung Ho Lee and Minoru Fukuda
5 Generation of Anti-sulfated Glycan Antibodies
Using Sulfotransferase-Deficient Mice............................... 51
Hiroto Kawashima
6 Fluorescent Microscopy as a Tool to Elucidate Dysfunction
and Mislocalization of Golgi Glycosyltransferases in COG Complex
Depleted Mammalian Cells....................................... 61
Rose A. Willett, Irina D. Pokrovskaya, and Vladimir V. Lupashin
7 A Practical Approach to Reconstruct Evolutionary
History of Animal Sialyltransferases and Gain Insights
into the Sequence-Function Relationships of Golgj-Glycosyltransferases..... 73
Daniel Petit, Roxana Elin Teppa, Jean-Michel Petit,
and Anne Harduin-Lepers
8 Fluorescent Lectin Staining of Drosophila Embryos
and Tissues to Detect the Spatial Distribution of Glycans
During Development........................................... 99
E Tian, Lipinjj Zhanjj, and Kelly G. Ten Hägen
9 Photoaffinity Labeling of Protein O-Mannosyltransferases
of the PMT1/PMT2 Subfamily.................................... 107
Martin Loibl and Sabine Strahl
10 Enzymatic Analysis of the Protein O-Glycosyltransferase,
Rumi, Acting Toward Epidermal Growth Factor-Like (EGF) Repeats....... 119
Hideyuki Takeuchi and Roberts. Haltiwanjjer
11 Enzymatic Characterization of Recombinant Enzymes
of O-GlcNAc Cycling........................................... 129
Eun Ju Kim and John A. Hanover
ix
x Contents
12 Antibodies and Activity Measurements for the Detection
of O-GlcNAc Transferase and Assay of its Substrate, UDP-GlcNAc......... 147
Tony Lefebvre, Ludivine Drougat, Stephanie Olivier-Van Stichelen,
Jean-Claude Michalski, and Anne-Sophie Vercoutter-Edouart
13 In Vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases........ 161
Mafias A. Musumeci, Maria V. Ielmini, and Mario F. Feldman
14 In Vitro UDP-Sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate
Transferase Assay and Product Detection by Thin Layer Chromatography.... 173
Kinnari B. Patel and Miguel A. Valvano
15 In Vitro O-Antigen Ligase Assay................................... 185
Xiang Kuan and Miguel A. Valvano
16 Functional Identification of Bacterial Glucosyltransferase WbdN........... 199
Tin Gao, Anna Vinnikova, and Inka Brockhausen
17 Methods for the Pasteurella Glycosaminoglycan Synthases:
Enzymes that Polymerize Hyaluronan, Chondroitin,
or Heparosan Chains............................................ 215
Paul L. DeAngelis
18 Methods for Measuring Class I Membrane-Bound Hyaluronan
Synthase Activity............................................... 229
Paul H. Weigel, Amy J. Padgett-McCue, and Bruce A Baggenstoss
19 Creation and Characterization of Glycosyltransferase Mutants
of Trypanosoma brucei........................................... 249
Luis Izquierdo, M. Lucia S. Gather, and Michael A. J. Ferguson
20 Mannose-6-Phosphate: A Regulator of LLO Destruction................ 277
Ningguo Gao and Mark A. Lehrman
21 N-Acetylglucosaminyltransferase (GnT) Assays
Using Fluorescent Oligosaccharide Acceptor Substrates:
GnT-III, IV, V, and IX (GnT-Vb)................................... 283
Shinji Takamatsu, Hiroaki Korekane, Kazuaki Ohtsubo,
Suguru Oguri, Jong Ti Park, Akio Matsumoto, and Naoyuki Taniguchi
22 A Method for Determination of UDP-GlcNAc:
GlcNAcß 1 -6(GlcNAcßl-2)Manal -R [GlcNAc to Man]
ßl -4^7-Acetylglucosaminyltransferase VI Activity....................... 299
Tomohiko Taguchi and Naoyuki Taniguchi
23 In Vitro Assays of Orphan Glycosyltransferases and
Their Application to Identify Notch Xylosyltransferases.................. 307
Maya K. Sethi, Falk F.R. Buettner, Angel Ashikov, and Hans Bakker
24 In Vitro Folding of ß-1,4Galactosyltransferase
and Polypeptide-cc- ^-Acetylgalactosaminyltransferase
from the Inclusion Bodies........................................ 321
Boopathy Ramakrishnan and Pradman K. Qasba
Contents xi
25 An Assay for al,6-Fucosyltransferase (FUT8) Activity Based
on the HPLC Separation of a Reaction Product
with Fluorescence Detection...................................... 335
Hideyuki Ihara, Hiroki Tsukamoto, Naoyuki Taniguchi,
and Yoshitaka Ikeda
26 Immunodetection of Glycosyltransferases in Gastrointestinal Tissues........ 349
Joana Gomes and Celso A. Reis
27 Glycosyltransferases in Chemo-enzymatic Synthesis of Oligosaccharides...... 357
Boris Tejsen and Irma van Die
28 Tumor Targeting by a Carbohydrate ligand-Mimicking Peptide........... 369
Shingo Hatakeyama, Toshiaki K. Shibata, Yuki Tobisawa,
Chikara Ohyama, Kazuhiro Sugihara, and Michiko N. Fukuda
29 Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease
Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation
Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity
Chromatography............................................... 387
Catharina Steentoft, Eric Paul Bennett, and Henrik Clausen
30 Generation of Monoclonal Antibodies to Native Active
Human Glycosyltransferases...................................... 403
Malene Bech Vester-Christensen, Eric Paul Bennett, Henrik Clausen,
and Ulla Mandel
Index
421
|
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| indexdate | 2024-07-10T00:40:17Z |
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| language | English |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Brockhausen, Inka 1944- Verfasser (DE-588)173090052 aut Glycosyltransferases methods and protocols ed. by Inka Brockhausen New York [u.a.] Humana Press 2013 XVII, 425 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1022 Springer protocols Erscheint auch als Online-Ausgabe 978-1-62703-465-4 Methods in molecular biology 1022 (DE-604)BV035362695 1022 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026106141&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Brockhausen, Inka 1944- Glycosyltransferases methods and protocols Methods in molecular biology |
| title | Glycosyltransferases methods and protocols |
| title_auth | Glycosyltransferases methods and protocols |
| title_exact_search | Glycosyltransferases methods and protocols |
| title_full | Glycosyltransferases methods and protocols ed. by Inka Brockhausen |
| title_fullStr | Glycosyltransferases methods and protocols ed. by Inka Brockhausen |
| title_full_unstemmed | Glycosyltransferases methods and protocols ed. by Inka Brockhausen |
| title_short | Glycosyltransferases |
| title_sort | glycosyltransferases methods and protocols |
| title_sub | methods and protocols |
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