Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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2012
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| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120523-1097169-1-7 Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | V, 118 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TECHNISCHE UNIVERSITAT MUNCHEN
LEHRSTUHL FUR PHYTOPATHOLOGIE
FUNCTIONAL ANALYSIS OF MEMBRANE TRAFFICKING PROTEINS IN THE
INTERACTION OF BARLEY WITH THE BARLEY POWDERY MILDEW FUNGUS
MAYA KATHARINA OSTERTAG
VOLLSTANDIGER ABDRUCK DER VON DER FAKULTAT WISSENSCHAFTSZENTRUM
WEIHENSTEPHAN FUR ERNAHRUNG, LANDNUTZUNG UND UMWELT DER TECHNISCHEN
UNIVERSITAT MUNCHEN ZUR ERLANGUNG DES AKADEMISCHEN GRADES EINES
DOKTORS DER NATURWISSENSCHAFTEN
GENEHMIGTEN DISSERTATION.
VORSITZENDER: UNIV.-PROF. DR. K. SCHNEITZ
PRUFER DER DISSERTATION: 1. UNIV.-PROF. DR. R. HUCKELHOVEN 2.
UNIV.-PROF. DR. J. DURNER
DIE DISSERTATION WURDE AM 13.02.2012 BEI DER TECHNISCHEN UNIVERSITAT
MUNCHEN EINGEREICHT UND DURCH DIE FAKULTAT WISSENSCHAFTSZENTRUM
WEIHENSTEPHAN FTIR ERNAHRUNG, LANDNUTZUNG UND UMWELT AM 02.05.2012
ANGENOMMEN.
HTTP://D-NB.INFO/1042738599
IMAGE 2
INDEX OF CONTENTS
1 INTRODUCTION 1
L.L THE PLANT IMMUNE SYSTEM 1
1.1.1 BASAL IMMUNITY - MAMP-RECOGNITION 1
1.1.1.1 MAMP-TRIGGCRCD DCFCNCE RESPONSES 2
1.1.1.2 EXAMPLES OF MAMP-RECOGNITION SYSTEMS 3
L. 1.2. EFFECTOR-TRIGGERED DEFENCE 4
1.1.2.1 DEFENCE REACTIONS FOLLOWING EFFECTOR PERCEPTION 6
1.1.2.2 EXAMPLES FOR THE INTERFERENCE OF EFFECTORS WITH PLANT DEFENCE 7
L .2 THE BARLEY - POWDERY MILDEW MODEL SYSTEM 8
1.3 THE CELLULAR TRANSPORT MACHINERY IN EUKARYOTES 9
1 .3. L PECULIARITIES OF TRANSPORT IN PLANT CELLS 11
1.3.2 SPECIFICITY OF TRANSPORT PROCESSES 12
1.3.2.1 INITIATION AND BUDDING: GENESIS OF VESICLES 12
1.3.2.2 MOVEMENT: DIRECTION OF THE VESICLE TO ITS DESTINATION 13 1.3.2.3
COAT RELEASE: PREPARATION FOR FUSION 14
1.3.2.4 VESICLE TARGETING AND FUSION 14
1.3.2.5 RAB-GTPASES 18
1.3.3 TRAFFICKING AND TRANSPORT IN PLANT-PATIIOGEN INTERACTIONS 19
1.3.3.1 GLOBAL CHANGES IN ATTACKED CCLLS 19
1.3.3.2 PLASMA MEMBRANE REACTIONS 19
1.3.3.3 VESICLE TRAFFICKING IN PLANT DEFENCE 22
1.3.3.4 ABC-TRANSPORTERS 23
1.3.3.5 SECRETORY MACHINERY AS TARGET FOR PATHOGEN MANIPULATION 24 1.4
OBJECTIVES 25
2 METHODS AND MATERIAL 27
2.1 PLANT MATERIAL, GROWTH CONDITIONS AND PATHOGENS 27 2.2 ISOLATION OF
BARLEY RNA AND GENERATION OF CDNA 28
2.3 SEMI QUANTITATIVE GENE EXPRESSION ANALYSIS 29
2.4 GENERATION OF CONSTRUCTS FOR TRANSIENT TRANSFORMATION OF BARLEY 30
2.4. L ORIGIN AND ISOLATION OF SEQUENCES USED FOR CLONING PROCEDURES 30
2.4.2 GENERATION OF RNAI CONSTRUCTS 34
2.4.3 GENERATION OF OVER-EXPRESSION CONSTRUCTS 36
2.4.4 CONSTRUCTS FOR TARGETED YEAST TWO HYBRID 37
2.4.5 GENERATION OF A DNHVYPTI-LIKE CONSTRUCT 38
2.5 GENERATION OF DOUBLE STRANDED RNA (DSRNA) 39
2.6 CONSTRUCTS FOR STABLE TRANSFORMATION OF BARLEY 40
2.7 YEAST TRANSFORMATION 40
2.8 TRANSIENT TRANSFORMATION OF BARLEY EPIDERMAL CELLS 42 2.9
MICROSCOPIC EVALUATION OF TRANSFORMED BARLEY EPIDERMAL CELLS 44 2.9.1
EVALUATION OF RNAI AND OVER-EXPRESSION EXPERIMENTS 44 2.9.2 EVALUATION
OF SUBCELLULAR LOCALIZATION 44
2.10 MICROSCOPIC EVALUATION OF ARABIDOPSIS T-DNA INSERTION LINES 45
3 RESULTS 46
3.1 SCREENING FOR CANDIDATES USING TRANSIENT GENE SILENCING 46 3.2
CANDIDATES SICNIFICANTLY INFLUENCING THE INTERACTION OF BARLEY WITH BGH
...... 49 3.2.1 A PREDICTED PROTEIN PUTATIVELY CONTAINING AN EX070
DOMAIN 49 3.2.2 TWO ADP RIBOSYLATION FACTOR 51
I
IMAGE 3
INDEX OF CONTENTS
3.2.3 THE PUTATIVE TETHERING COMPLEX SUBUNIT HVCOG3 55
3.3 INVESTIGATIONS ON HVCOG3 EFFECTS IN BARLEY 56
3.3.1 EFFECTS OF HVCOG3 RNAI ON BARLEY EPIDERMAL CELL FUNCTION 58 3.4
INVESTIGATIONS ON THE COG COMPLEX OF BARLEY 60
3.4.1 IN SILICO STUDIES 60
3.4.2 FUNCTIONAL CHARACTERIZATION OF THE DIFFERENT HVCOG SUBUNITS 62
3.4.3 GENE EXPRESSION ANALYSIS OF HVCOG SUBUNITS 63
3.5 INVESTIGATIONS ON POTENTIAL INTERACTION PARTNERS OF HVCOG3 65 3.5.1
FUNCTIONAL ANALYSIS OF POTENTIAL HVCOG3 INTERACTION PARTNERS 65 3.5.2
FURTHER INVESTIGATIONS ON HVCOPIY-UKE, HVYPTI-UKE AND HVVTII-UKE 67
4 DISCUSSION 71
4.1 IDENTIFICATION OF PROMISING CANDIDATES USING TIGS 71
4.2 CANDIDATE GENES, WHICH INFLUENCE THE SUSCEPTIBILITY OF BARLEY TO BGH
72 4.2.1 A PUTATIVE EXOCYST SUBUNIT 72
4.2.2 ADPRIBOSYLATION FACTORS 75
4.2.3 COG3, THE COG COMPLEX AND POTENTIALLY ASSOCIATED PROTEINS 78
5 SUMMARY 88
6 ZUSAMMENFASSUNG 89
7 REFERENCES 90
8 APPENDIX 111
II
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| spelling | Ostertag, Maya Katharina Verfasser (DE-588)1042738386 aut Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus Maya Katharina Ostertag 2012 V, 118 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache München, Techn. Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20120523-1097169-1-7 http://mediatum.ub.tum.de/node?id=1097169 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20120523-1097169-1-7 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026090162&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ostertag, Maya Katharina Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus |
| subject_GND | (DE-588)4113937-9 |
| title | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus |
| title_auth | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus |
| title_exact_search | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus |
| title_full | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus Maya Katharina Ostertag |
| title_fullStr | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus Maya Katharina Ostertag |
| title_full_unstemmed | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus Maya Katharina Ostertag |
| title_short | Functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus |
| title_sort | functional analysis of membrane trafficking proteins in the interaction of barley with the barley powdery mildew fungus |
| topic_facet | Hochschulschrift |
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