The crystal structure of splice variant mutant of arrestin, p44: the pre-active confirmation of arrestin
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 95 Bl. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Kim, Yong Ju |d 1975- |e Verfasser |0 (DE-588)1036483770 |4 aut | |
| 245 | 1 | 0 | |a The crystal structure of splice variant mutant of arrestin, p44 |b the pre-active confirmation of arrestin |c von Yong Ju Kim |
| 264 | 1 | |c 2013 | |
| 300 | |a 95 Bl. |b Ill., graph. Darst. | ||
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| 502 | |a Berlin, Humboldt-Univ., Diss., 2013 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Reproduziert als |a Kim, Yong Ju, 1975- |t The crystal structure of splice variant mutant of arrestin, p44 |c the pre-active confirmation of arrestin |d Ketsch bei Mannheim : Mikroform Dissertation, 2013 |h 1 Mikrofiche |w (DE-604)BV041200183 |
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Datensatz im Suchindex
| _version_ | 1804150493965975552 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
PAGE
ABSTRACT IN GERMAN 1
ABSTRACT IN ENGLISH 2
LISTS OF FIGURES E
LISTS OF TABLES 7
ABBREVIATIONS 8
PUBLICATIONS 10
1. INTRODUCTION 11
1.1. VISUAL SIGNAL TRANSDUCTION 11
1.1.1. ROD AND CONE CELLS 11
1.1.2. ACTIVATION CASCADE 12
1.1.3. DEACTIVATION CASCADE 13
1.2. GPCR 16
1.2.1. RHODOPSIN 16
1.2.2. STRUCTURAL INSIGHT FROM THE INACTIVE CONFORMATION OF R H O D O P
S I N - 1 8
1.2.3. ACTIVATION OF RHODOPSIN 20
1.3. ARRESTIN 22
1.3.1. FUNCTIONS OF ARRSTIN 22
1.3.2. STRUCTURAL INSIGHT ON ARRESTINS 22
1.3.3. PHOSPHATE SENSOR (PHOSPHORYLATION-RECOGNITION SITE) OF ARRESTIN
24
1.3.4. ACTIVATION SENSOR (BINDING-RECOGNITION SITE) OF ARRESTIN 25
1.3.5. ACTIVATION MECHANISM OF ARRESTIN 26
1.3.6. SPLICE VARIANT MUTANT OF ARRESTIN, P44 27
1.4. THESIS OBJECTIVE 28
2. MATERIALS AND METHODS 2 9
2.1. MATERIALS 29
2.1.1. CHEMICALS AND TOOLS 29
HTTP://D-NB.INFO/1037140818
IMAGE 2
2.1.2. SOFTWARE AND HARDWARE 29
2.1.3. EQUIPMENTS 30
2.1.4. MATERIALS FOR PREPARATION AND CRYSTALLIZATION OF P44 30
2.1.5. INFORMATION ABOUT CMC (CRITICAL MICELLE CONCENTRATION) VALUES OF
DETERGENTS 31
2.1.6. BUFFERS FOR PROTEIN PURIFICATION 32
2.2. METHODS 32
2.2.1. PREPARATION OF ROD OUTER SEGMENT FROM RETINA 32
2.2.2. PURIFICATION OF OPSIN FROM ROS MEMBRANES 33
2.2.3. PREPARATION OF PHORPHORYLATED RHODOPSIN 33
2.2.4. PURIFICATION OF P44 34
2.2.5. SPECTROPHOTOMETRICAL ANALYSIS OF P44 34
2.2.6. CONCENTRATION-DEPENDENT SELF ASSOCIATION OF ARRESTIN AND P44 IN
SOLUTION 35
2.2.7. PULLDOWN ASSAY 35
2.2.8. CRYSTALLIZATION OF P44 35
2.2.9. DATA COLLECTION 36
2.2.10. CALCULATION OF ELECTRON DENSITY MAP AND MODEL BUILDING 36
2.2.11. REFINEMENT 36
2.2.12. VALIDATION AND MODEL DEPOSITION 37
3. RESULTS AND DISCUSSION 3 8
3.1. EXPRESSION OF P44 IN ESCHERICHIA COLI 38
3.2. PURIFICATION OF P44 39
3.3. CHECKING OF THE EXTRA METALL BETWEEN P44 AND/OR ARRESTIN 40
3.4. CONCENTRATION-DEPENDENT SELF ASSOCIATION OF ARRESTIN AND/OR P44 IN
SOLUTION 43
3.5. CENTRIFUGAL PULLDOWN ASSAY 45
3.6. CRYSTALLIZATION AND DATA COLLECTION OF P44 47
3.7. STRUCTURE SOLUTION AND REFINEMENT 49
3.8. VALIDATION AND MODEL DEPOSITION 51
3.9. OVERALL STRUCTURE OF THE P44 52
3.10. PHOSPHATE SENSOR (PHOSPHORYLATION-RECOGNITION SITE) 56
3.10.1 THE ACTIVATION OF ARRESTIN 56
3.10.2 POLAR CORE 58
3.11. ACTIVATION SENSOR (BINDING RECOGNITION SITE) AND CONFORMATIONAL
IMAGE 3
REARRANGEMENT IN INTER-DOMAIN
3.12. SEQUENCE SIMILARITIES AND CONSERVED MOTIFS (POLAR CORE,
YKS(N)D(A), T(M)QGL) WITHIN ARRESTINS
3.12.1. POLAR CORE
3.12.2. NEW HYDROGEN BONDING NETWORK AT THE INTER-DOMAIN:
YKS(N)D(A) AND T(M)QGL MOTIFS
3.13. ACTIVATION MECHANISM OF ARRESTIN
3.14. MODEL OF P-RHO*/RHO* P44 COMPLEX
4. CONCLUSIONS
5. BIBLIOGRAPHY
ACKNOWLEDGEMENT EIDESSTATTLICHE ERKLARUNG
|
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| author | Kim, Yong Ju 1975- |
| author_GND | (DE-588)1036483770 |
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| building | Verbundindex |
| bvnumber | BV041108186 |
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| indexdate | 2024-07-10T00:39:48Z |
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| language | English |
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| spelling | Kim, Yong Ju 1975- Verfasser (DE-588)1036483770 aut The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin von Yong Ju Kim 2013 95 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Humboldt-Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content Reproduziert als Kim, Yong Ju, 1975- The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin Ketsch bei Mannheim : Mikroform Dissertation, 2013 1 Mikrofiche (DE-604)BV041200183 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026084451&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kim, Yong Ju 1975- The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin |
| subject_GND | (DE-588)4113937-9 |
| title | The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin |
| title_auth | The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin |
| title_exact_search | The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin |
| title_full | The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin von Yong Ju Kim |
| title_fullStr | The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin von Yong Ju Kim |
| title_full_unstemmed | The crystal structure of splice variant mutant of arrestin, p44 the pre-active confirmation of arrestin von Yong Ju Kim |
| title_short | The crystal structure of splice variant mutant of arrestin, p44 |
| title_sort | the crystal structure of splice variant mutant of arrestin p44 the pre active confirmation of arrestin |
| title_sub | the pre-active confirmation of arrestin |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026084451&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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