Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | XII, 139 Bl. graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro |c vorgelegt von Ines Möller |
| 246 | 1 | 3 | |a Optimierung humaner, aus Monozyten differenzierter, dendritischer Zellen für die Aufnahme und Präsentation von Tumorantigenen in vitro |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENTS
CONTENTS
PUBLICATIONS V
RECORD OF ACHIEVEMENT VI
ACKNOWLEDGMENT VIII
SUMMARY 13
ZUSAMMENFASSUNG 15
1. INTRODUCTION 17
1.1. IMMUNOTHERAPY 17
1.1.1. PASSIVE IMMUNOTHERAPY 17
1.1.2. ACTIVE IMMUNOTHERAPY 18
1.2. DENDRITIC CELL DEVELOPMENT 20
1.2.1. IN VITRO MATURATION OF DCS 22
1.2.2. PHENOTYPE 23
1.3. INTERACTION ABILITIES 24
1.3.1. CHEMOKINES 24
1.3.2. INTERLEUKINS 25
1.3.3. TH1/TH2 POLARIZATION 26
1.3.4. INDOLEAMINE 2,3-DIOXYGENASE 29
1.4. FUNCTIONALITY 30
1.4.1. ANTIGEN UPTAKE 30
1.4.2. CROSS-PRESENTATION 35
1.4.3. MIGRATION 37
1.4.4. T-CELL PRIMING 37
1.5. T-CELL TOLERANCE 38
1.6. CANCER BIOLOGY 39
1.7. TUMOR ANTIGENS 40
2. AIM 42
3. MATERIAL AND METHODS 43
3.1. CHEMICALS AND REAGENTS 43
3.1.1. CHEMICALS 43
HTTP://D-NB.INFO/1036298426
IMAGE 2
CONTENTS
3.1.2. CELL-LINES 44-
3.1.3. ANTIBODIES 44
3.1.4. MEDIA AND SUPPLEMENTS 46
3.1.5. PEPTIDES, PENTAMERS AND PROTEINS 48
3.1.6. KITS 49
3.1.7. PLASTICWARE 49
3.1.8. SOFTWARE 50
3.1.9. TECHNICAL EQUIPMENT 51
3.1.10. MEDIA AND BUFFERS 52
3.2. CELL CULTURE 53
3.2.1. FREEZING AND THAWING CELLS 53
3.2.2. CELL-LINES 54
3.2.3. PRIMARY CELLS 54
3.3. MOLECULAR METHODS 56
3.3.1. RNA EXTRACTION 56
3.3.2. MICROARRAY 57
3.4. IMMUNOFLUORESCENCE 58
3.4.1. FLOW CYTOMETRY 58
3.4.2. IMAGING CYTOMETRY 59
3.5. PROTEIN BIOCHEMICAL METHODS 60
3.5.1. CELL LYSIS 60
3.5.2. QUANTITATIVE PROTEIN DETERMINATION 61
3.5.3. SEMI-QUANTITATIVE AND QUALITATIVE ANALYSIS 62
3.5.4. ANTIGEN PREPARATION 64
3.6. FUNCTIONAL ASSAYS 67
3.6.1. MIGRATION 67
3.6.2. UPTAKE 68
3.6.3. BLOCKADE OF ANTIGEN INCORPORATION 69
3.6.4. INDUCTION OF ANTIGEN-SPECIFIC T LYMPHOCYTES 69
3.7. STATISTICS 73
4. RESULTS 74
4.1. IMMUNOPHENOTYPE OF DIFFERENTIALLY MATURED DCS 74
4.2. GENE EXPRESSION PROFILES 77
II
IMAGE 3
CONTENTS
4.3. CHEMOKINES: SECRETION AND MIGRATION ABILITIES 81
4.4. SECRETION OF IL-10 AND IL-12 82
4.5. INDUCTION OF IDO 85
4.6. PRIMING OF NAIVE MART 26 .35MOD-SPECIFIC T CELLS 86
4.6.1. PENTAMER ASSAY AND IFNY SECRETION :..86
4.7. DELIVERY OF CELL-DERIVED ANTIGEN 89
4.7.1. FACILITATION OF PHAGOCYTOSIS BY DCS 89
4.7.2. INFLUENCE OF DIFFERENT PRETREATED CELLS ON PHAGOCYTOSIS BY DCS 91
4.7.3. INTERACTION BETWEEN HSP90 AND CD91 93
4.8. DELIVERY OF SYNTHETIC ANTIGEN 94
4.8.1. PREPARATION OF ANTIGEN 94
4.8.2. BINDING PROPERTIES OF ANTI-MAGE ANTIBODIES 96
4.8.3. INFLUENCE OF DIFFERENT RHMAGE-A3 PROTEIN FORMULATIONS ON
ENDOCYTOSIS
BY DCS 98
4.9. PRIMING OF NAIVE MAGE-A3-SPECIFIC T CELLS 102
4.9.1. CROSS-PRESENTATION OF MAGE-A3 ANTIGENS BY MATURE DCS STIMULATES
NAIVE CD4 + AND CD8 + T CELLS 104
5. DISCUSSION 106
5.1. DIFFERENTIAL MATURATION OF MONOCYTE DERIVED DCS 106
5.1.1. RELEVANT GENES FOR T-CELL PRIMING ARE DIFFERENTIALLY EXPRESSED
BETWEEN
POLY(L:C)- AND PGE 2 -BASED DCS 106
5.1.2. POLY(L:C) CYTOKINES MEDIATE TH1 POLARIZATION BY IL-12 107
5.1.3. DC MATURATION WITH POLY(L:C) INDUCES STRONG EXPRESSION AND
ENZYMATIC
ACTIVITY OF IDO 108
5.1.4. BOTH MATURATION PROTOCOLS INDUCE DCS THAT EFFICIENTLY PRIME NAIVE
T
CELLS USING PEPTIDES 109
5.1.5. MIGRATORY ABILITY DEPENDS ON PGE 2 EXPOSURE 110
5.1.6. DC FEATURES FOR IN VITRO AND IN VIVO APPROACHES INDUCED BY
POLY(L:C)-AND
PGE 2 -BASED MATURATION 111
5.2. CHARACTERIZATION OF CELL-DERIVED AND RECOMBINANT ANTIGENS 111
5.2.1. BORTEZOMIB PRETREATMENT ENHANCES APOPTOSIS AND U266 CELL
PHAGOCYTOSIS BY DCS 112
III
IMAGE 4
CONTENTS
5.2.2. OPSONIZATION OF RHMAGE-A3 PROTEIN FOR RECEPTOR-MEDIATED
ENDOCYTOSIS
BY DCS 113
5.2.3. INFLUENCE OF DIFFERENT MAGE-A3 PROTEIN FORMULATION ON ENDOCYTOSIS
BY
DCS 115
5.2.4. EFFICIENT PRIMING OF NAIVE T CELLS AGAINST MAGE-A3 ANTIGENS 117
5.3. CONCLUSION 119
5.4. OUTLOOK 119
6. SUPPLEMENTARY DATA 121
7. FIGURES 123
8. TABLES 125
9. REFERENCES 126
IV
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| indexdate | 2024-07-10T00:39:19Z |
| institution | BVB |
| language | English German |
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| physical | XII, 139 Bl. graph. Darst. |
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| spelling | Möller, Ines Verfasser aut Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro vorgelegt von Ines Möller Optimierung humaner, aus Monozyten differenzierter, dendritischer Zellen für die Aufnahme und Präsentation von Tumorantigenen in vitro 2012 XII, 139 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache Freiburg (Breisgau), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026064600&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Möller, Ines Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro |
| subject_GND | (DE-588)4113937-9 |
| title | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro |
| title_alt | Optimierung humaner, aus Monozyten differenzierter, dendritischer Zellen für die Aufnahme und Präsentation von Tumorantigenen in vitro |
| title_auth | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro |
| title_exact_search | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro |
| title_full | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro vorgelegt von Ines Möller |
| title_fullStr | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro vorgelegt von Ines Möller |
| title_full_unstemmed | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro vorgelegt von Ines Möller |
| title_short | Optimization of human monocyte-derived dendritic cells for uptake and presentation of tumor antigens in vitro |
| title_sort | optimization of human monocyte derived dendritic cells for uptake and presentation of tumor antigens in vitro |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026064600&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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