Identification of epithelial progenitor cells in the adult thymus:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in engl. und dt. Sprache |
| Beschreibung: | IV, 117 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENTS
CONTENTS
ZUSAMMENFASSUNG I
SUMMARY II
CONTENTS I
1 INTRODUCTION 1
1 . 1 EVOLUTION OF ADAPTIVE IMMUNITY 2
1 . 2 THYMOCYTE MATURATION IN THE THYMUS 3
1.2.1 THYMOCYTE SELECTION AND CENTRAL TOLERANCE 3
1.2.2 TCR SELECTION MODEL 6
1 . 3 THYMUS DEVELOPMENT 8
1.3.1 ONTOGENY O F THE THYMUS 8
1.3.2 COMMON TEC PROGENITORS 10
1.3.3 CTEC PROGENITOR 11
1.3.4 MTECPROGENITORS AND DEVELOPMENT 12
1 . 4 ATROPHY OF THE THYMUS AND IMPLICATIONS FOR THE IMMUNE SYSTEM 1 5
1 . 5 ADULT EPITHELIAL STEM CELLS AND PROGENITORS 1 5
1.5.1 METHODS TO ISOLATE TISSUE-SPECIFIC STEM CELLS 16
1.5.2 COMMON SURFACE MARKER O F EPITHELIAL STEM CELLS 18
1 . 6 OBJECTIVE OF THIS STUDY 2 1
2 MATERIAL AND METHODS 22
2 . 1 MATERIAL 2 2
2.1.1 CHEMICALS 22
2.1.2 ENZYMES AND PROTEINS 23
2.1.3 MEDIA, BUFFERS AND COMMERCIAL SOLUTIONS 23
2.1.4 MICROBEADS USED F O R MACS PURIFICATION 27
2.1.5 ANTIBODIES 28
2.1.6 PRIMER 29
2.1.7 MICROARRAY AND COMMERCIAL KITS 29
2.1.8 CONSUMABLES 30
2.1.9 EQUIPMENT 31
2.1.10 SOFTWARE 32
2.1.11 MOUSE STRAINS 33
2 . 2 METHODS 3 4
2.2.1 ISOLATION O F GENOMIC DNA FROM MURINE TAILS 3 4
2.2.2 CONVENTIONAL PCR 34
2.2.3 ISOLATION O F MURINE MEDULLARY THYMIC EPITHELIAL CELLS 34
2.2.4 ENRICHMENT O F TECS USING MACS-MICROBEADS 35
2.2.5 FACS STAINING O F MTEC PROGENITORS 36
2.2.6 THYMOSPHERE ASSAY 37
2.2.7 IN VITRO DIFFERENTIATION 39
2.2.8 REAGGREGATION THYMIC ORGAN CULTURES (RTOC) 4 0
PAGE | III
HTTP://D-NB.INFO/1037048962
IMAGE 2
CONTENTS
2.2.9 FETAL THYMIC ORGAN CULTURE (FTOC): MICROINJECTION OFMTEC
PROGENITORS 41
2.2.10 TRANSPLANTATION OFFTOCS AND RTOCS UNDER THE KIDNEY CAPSULE 43
2.2.11 IMMUNOHISTOCHEMISTRY 49
2.2.12 RNA PREPARATION 50
2.2.13 MICROARRAY ANALYSIS 50
2.2.14 LABEL-RETAINING ASSAY 51
2.2.15 PKH26-LABELING 51
2.2.16 CELL SURFACE MARKER SCREENING 52
3 RESULTS 53
3 . 1 FOUR NEW M T E C PROGENITOR SUBSETS 5 3
3.1.1 IDENTIFICATION O F 4 NEW SUBSETS WITHIN THE IMMATURE MTEC
COMPARTMENT 53
3.1.2 SUBPOPULATION DYNAMICS DURING ONTOGENY 55
3.1.3 GENE EXPRESSION ANALYSIS O F THE 4 SUBPOPULATIONS 56
3.1.4 DIFFERENTIATION POTENTIAL O F THE F O U R SUBSETS IN RTOCS: INTER
- CONVERSION 57
3.1.5 DIFFERENTIATION POTENTIAL O F THE F O U R IMMATURE SUBPOPULATIONS
IN VIVO 60
3.1.6 LABEL-RETENTION: TWO SUBSETS CONTAIN GFP RETAINING CELLS 63
3 . 2 THYMOSPHERES 6 5
3.2.1 IDENTIFICATION O F THYMOSPHERES FORMING CELLS IN THE ADULT THYMUS
65
3.2.2 CHARACTERIZATION O F SPHERE-FORMING CELLS IN THE ADULT THYMUS 66
3.2.3 DIFFERENTIATION POTENTIAL O F THYMOSPHERE FORMING CELLS IN VITRO
AND EX VIVO 7 7
3.2.4 DIFFERENTIATION POTENTIAL O F THYMOSPHERE FORMING CELLS IN VIVO 80
3.2.5 THYMOSPHERES FORMING CELLS IN EMBRYONIC AND AGED THYMUS 81
4 DISCUSSION 83
4 . 1 IDENTIFICATION OF A PUTATIVE THYMIC STEM CELL 8 3
4.1.1 THE SPHERE-FORMING ASSAY - AN ALTERNATIVE APPROACH TO IDENTIFY
STEM CELLS IN THE THYMUS 84
4.1.2 PHENOTYPIC CHARACTERIZATION O F ADULT TEC STEM CELL 87
4 . 2 STEM CELL MARKERS IDENTIFY NEW SUBSETS OF M T E C PROGENITORS 9 0
4.2.1 A CO-LINEAR DEVELOPMENTAL SEQUENCE 91
4.2.2 SLOWLY CYCLING CELLS IN TWO SUBSETS OFMTEC PROGENITORS 94
4 . 3 THE ROADMAP OF T E C DEVELOPMENT REVISITED 9 5
4.3.1 A THERAPEUTIC POTENTIAL F O R THYMIC STEM CELLS? 96
4 . 4 CONCLUDING REMARKS AND FUTURE PERSPECTIVES 9 7
5 REFERENCES 98
6 LIST OF ABBREVIATION 115
7 ACKNOWLEDGMENT 117
PAGE | IV
|
| any_adam_object | 1 |
| author | Klug, Paula |
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| author_facet | Klug, Paula |
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| ctrlnum | (OCoLC)854726305 (DE-599)BSZ383025842 |
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| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.966 |
| dewey-search | 571.966 |
| dewey-sort | 3571.966 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| language | English German |
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| spelling | Klug, Paula Verfasser (DE-588)1035438828 aut Identification of epithelial progenitor cells in the adult thymus [vorgelegt von Paula Theresa Klug] 2013 IV, 117 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in engl. und dt. Sprache Heidelberg, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026060151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Klug, Paula Identification of epithelial progenitor cells in the adult thymus |
| subject_GND | (DE-588)4113937-9 |
| title | Identification of epithelial progenitor cells in the adult thymus |
| title_auth | Identification of epithelial progenitor cells in the adult thymus |
| title_exact_search | Identification of epithelial progenitor cells in the adult thymus |
| title_full | Identification of epithelial progenitor cells in the adult thymus [vorgelegt von Paula Theresa Klug] |
| title_fullStr | Identification of epithelial progenitor cells in the adult thymus [vorgelegt von Paula Theresa Klug] |
| title_full_unstemmed | Identification of epithelial progenitor cells in the adult thymus [vorgelegt von Paula Theresa Klug] |
| title_short | Identification of epithelial progenitor cells in the adult thymus |
| title_sort | identification of epithelial progenitor cells in the adult thymus |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026060151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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