Functional regulation of the survival of motor neurons-complex:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in engl. und dt. Sprache |
| Beschreibung: | 104 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Functional regulation of the survival of motor neurons-complex |c presented by Björn Boysen |
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| 300 | |a 104 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
1. INTRODUCTION 1
1.1 SNRNP BIOGENESIS - T H E JOURNEY STARTS IN T H E NUCLEUS 1
1.2 THE SMN COMPLEX - KEY PLAYER IN SNRNP BIOGENESIS 2
1.3 SNRNP BIOGENESIS IN T H E CYTOPLASM 5
1.4 THE FINAL STEPS O F SNRNP BIOGENESIS OCCUR IN T H E NUCLEUS 9
1.5 NUCLEAR ORGANIZATION 9
1.5.1THE CAJAL BODY 1 1
1.6 REGULATION O F T H E SMN COMPLEX 1 4
1.7 AIMS O F THIS THESIS 15
2. RESULTS 17
2.1 GENERATION A N D CHARACTERISATION O F A SPECIFIC UNRIP ANTIBODY 17
2.2 REGULATION O F UNRIP ASSOCIATION WITH T H E SMN COMPLEX VIA
PHOSPHORYLATION 1 8
2.3 DO INTERACTION PARTNERS INFLUENCE UNRIP BINDING T O T H E SMN
COMPLEX? 2 0
2.4 DYNAMICS O F UNRIP INTERACTION WITH T H E SMN-COMPLEX 2 3
2.5 DOES A NUCLEAR POOL O F UNRIP EXIST? 2 5
2.6 LOSS O F UNRIP RESULTS IN CYTOPLASMIC SMN ACCUMULATIONS 2 6
2.7 UNRIP D O E S N O T FUNCTION A S A MAJOR INHIBITOR O F P R E M A T U
R E SMN ACCUMULATION 2 8
2.8 SMN-SNRNP INTERMEDIATES ACCUMULATE PRIOR T O 5 CAP HYPERMETHYLATION
2 9
U P O N UNRIP KNOCKDOWN
2.9 UNRIP FUNCTIONS IN 5 CAP HYPERMETHYLATION O F SNRNAS 3 1
2.10 RNAI-BASED SCREENING FOR KINASES T H A T INFLUENCE SNRNP BIOGENESIS
3 5
2.11 VALIDATION O F SCREEN NARROWS DOWN T H E LIST O F PUTATIVE
CANDIDATES 3 9
HTTP://D-NB.INFO/1037255917
IMAGE 2
2.12 ANALYSIS O F PUTATIVE CANDIDATES BY SECONDARY SCREENS 4 1
2.13 KNOCKDOWN O F MTOR D O E S N O T IMPAIR LOCALIZATION O F O T H E R
SNRNP A N D 5 0
SPLICING MARKERS
2.14 ANALYSIS O F SMN PHOSPHORYLATION S T A T E U P O N MTOR A N D S6K1
KNOCKDOWN 5 2
2.15 RAPAMYCIN T R E A T M E N T CONFIRMS SMN MISLOCALISATION 5 3
2.16 OVEREXPRESSION O F MTOR CAUSES A N INCREASE IN NUCLEAR SMN FOCI 5 5
2.17 THE SMN P H E N O T Y P E IS LARGELY I N D E P E N D E N T O F
PROTEIN TRANSLATION INHIBITION 5 6
3. DISCUSSION 60
3.1 INVESTIGATING CIS REGULATION - T H E SMN-COMPLEX A N D ITS C O M P
O N E N T UNRIP 6 1
3.2 INVESTIGATING TRANS REGULATION - T H E SMN-COMPLEX A N D
PHOSPHORYLATION 6 7
4. MATERIALS AND METHODS 74
4.1 MATERIALS 7 4
4.1.1 REAGENTS 7 4
4.1.2 ANTIBIOTICS 7 4
4.1.3 CELL CULTURE MEDIA A N D ADDITIVES 7 4
4.1.4 CELLS 7 5
4.1.5 ANTIBODIES 7 5
4.1.6 ENZYMES 7 5
4.1.7 DOUBLE STRANDED OLIGONUCLEOTIDES 7 6
4.1.8 SOLUTIONS A N D BUFFERS 7 7
4.2 M E T H O D S 7 9
4.2.1 CLONING 7 9
4.2.2 PURIFICATION O F RECOMBINANT UNRIP 7 9
IMAGE 3
4.2.3 PURIFICATION O F ANTIBODIES 8 0
4.2.4 SDS-GEL ELECTROPHORESIS A N D WESTERN BLOT 8 0
4.2.5 TWO-DIMENSIONAL GEL ELECTROPHORESIS 8 0
4.2.6 TRANSFECTION 8 1
4.2.7 IMMUNOSTAINING FOR MICROSCOPY 8 1
4.2.8 IMMUNOPRECIPITATION 8 2
4.2.9 IN VITRO TRANSCRIPTION O F U1 SNRNA 8 2
4.2.10 USNRNP ASSEMBLY ASSAY 8 3
4.2.11 TMG-ASSAY 8 3
4.2.12 POLYSOME PROFILES 8 3
5. REFERENCES 84
6. APPENDICES 96
6.1 SUPPLEMENTAL DATA 9 6
6.1.1 INTERACTING PROTEINS O F UNRIP 9 6
6.2 ABBREVATIONS 9 8
6.3 LIST O F FIGURES 1 0 1
6.4 LIST O F TABLES 102
DANKSAGUNG 103
EIDESSTATTLICHE ERKLARUNG 104
|
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| language | English German |
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| spelling | Boysen, Björn Verfasser (DE-588)1035390957 aut Functional regulation of the survival of motor neurons-complex presented by Björn Boysen 2013 104 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in engl. und dt. Sprache Heidelberg, Univ., Diss, 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026060081&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Boysen, Björn Functional regulation of the survival of motor neurons-complex |
| subject_GND | (DE-588)4113937-9 |
| title | Functional regulation of the survival of motor neurons-complex |
| title_auth | Functional regulation of the survival of motor neurons-complex |
| title_exact_search | Functional regulation of the survival of motor neurons-complex |
| title_full | Functional regulation of the survival of motor neurons-complex presented by Björn Boysen |
| title_fullStr | Functional regulation of the survival of motor neurons-complex presented by Björn Boysen |
| title_full_unstemmed | Functional regulation of the survival of motor neurons-complex presented by Björn Boysen |
| title_short | Functional regulation of the survival of motor neurons-complex |
| title_sort | functional regulation of the survival of motor neurons complex |
| topic_facet | Hochschulschrift |
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