Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-157666 Inhaltsverzeichnis |
| Beschreibung: | VI, 88 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Daniel, Guillaume |d 1984- |e Verfasser |0 (DE-588)1035619245 |4 aut | |
| 245 | 1 | 0 | |a Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis |c von Guillaume Daniel |
| 264 | 1 | |c 2012 | |
| 300 | |a VI, 88 Bl. |b Ill., graph. Darst. | ||
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| 502 | |a München, Univ., Diss., 2012 | ||
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Datensatz im Suchindex
| _version_ | 1804150447650373632 |
|---|---|
| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE O F CONTENTS I
LIST O F FIGURES ILL
LIST O F TABLES IV
LIST O F ABBREVIATIONS V
ABSTRACT 1
INTRODUCTION 2
I. FORMATION OF THE CENTRAL NERVOUS SYSTEM (CNS) 3
II. NEURAL STEM CELLS AND NEURAL PROGENITORS 4
A) RADIAL GLIAL CELLS 4
B) INTERMEDIATE PROGENITOR CELLS 5
C) PRONEURAL FACTORS AND E-PROTEINS 6
D) REPRESSORS O F NEUROGENESIS AND MAINTENANCE OF PLURIPOTENCY BY HES
AND ID PROTEINS 9
III. BRAIN DEVELOPMENT AND IMPRINTED GENES 11
IV. GENOMIC IMPRINTING PLAYS KEY ROLES IN BRAIN FUNCTION 12
V. THE PATERNALLY EXPRESSED GENE ZACL AS REGULATOR O F EMBRYONIC
DEVELOPMENT 14
A) ZAC7/LOTL/PLAGLL PROTEIN STRUCTURE 14
B) EXPRESSION PATTERN OF ZACL 16
C) IMPRINTING O F ZACL 17
D) BIOLOGICAL FUNCTION O F ZACL 18
E) ZACL AS REGULATOR O F PROGENITOR CELL DIFFERENTIATION 21
AIM O F THE THESIS 22
MATERIALS AND METHODS 23
I. MATERIALS 23
A) MOLECULAR BIOLOGY 23
B) CELL CULTURE MATERIALS 24
C) ANTIBODIES 24
II. METHODS 25
A) ANIMALS 25
B) DNA ANALYSIS 25
C) RNA ANALYSIS 26
HTTP://D-NB.INFO/1037404971
IMAGE 2
D) PLASMIDS 28
E) RECOMBINANT PROTEIN CONSTRUCT 30
E) PLASMID PREPARATION 31
F) CELL CULTURE AND TRANSFECTION EXPERIMENTS 32
G) LUCIFERASE ASSAY 36
H) PROTEIN PREPARATION 36
I) ANTIBODY PRODUCTION 37
J) WESTERN BLOTS 37
K) IMMUNOHISTOCHEMISTRY 38
F) CHROMATIN IMMUNOPRECIPITATION (CHIP) 39
1) STATISTICAL ANALYSIS AND COMPUTER SOFTWARE 39
RESULTS 40
I. ZACL INDUCES EXPRESSION O F TCF4 IN THE NEURAL STEM CELL LINE C17.2
42
II. ZACL REGULATES TCF4 GENE DURING NEURONAL DIFFERENTIATION OF MOUSE
EMBRYONIC STEM CELLS 46
III. ZACL REGULATES TCF4 EXPRESSION DURING EMBRYONIC NEUROGENESIS 53
IV. ZACL, TCF4 AND P57 KIP2 ARE CO-REGULATED DURING BRAIN DEVELOPMENT 58
V. ZACL REGULATES TCF4 EXPRESSION DURING ADULTHOOD 60
VI. ZAC1 MIGHT REGULATE TCF4 EXPRESSION IN HUMAN 63
VII. ANTIBODY VALIDATION 65
DISCUSSION 68
I. THE IMPRINTED GENE ZACL REGULATES THE EXPRESSION O F THE PRONEURAL
FACTOR TCF4 69
II. ZACL AS REGULATOR O F CELL DIFFERENTIATION 70
III. FUTURE DIRECTIONS: ZACL, TCF4 AND SCHIZOPHRENIA 72
CONCLUSION 75
REFERENCES 76
ACKNOWLEDGEMENTS 87
CURRICULUM VITAE 88
I I
|
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| spelling | Daniel, Guillaume 1984- Verfasser (DE-588)1035619245 aut Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis von Guillaume Daniel 2012 VI, 88 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-157666 http://edoc.ub.uni-muenchen.de/15766/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-157666 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026054927&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Daniel, Guillaume 1984- Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis |
| subject_GND | (DE-588)4113937-9 |
| title | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis |
| title_auth | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis |
| title_exact_search | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis |
| title_full | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis von Guillaume Daniel |
| title_fullStr | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis von Guillaume Daniel |
| title_full_unstemmed | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis von Guillaume Daniel |
| title_short | Tcf4 is a target gene of the imprinted gene Zac1 during mouse neurogenesis |
| title_sort | tcf4 is a target gene of the imprinted gene zac1 during mouse neurogenesis |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/15766/ https://nbn-resolving.org/urn:nbn:de:bvb:19-157666 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026054927&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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