Kinetics of protein damage during evaporative drying of levitated microdroplets:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Volltext Volltext http://d-nb.info/1034425501/34 Inhaltsverzeichnis |
| Beschreibung: | VIII, 192 S. Ill., graph. Darst. |
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Datensatz im Suchindex
| _version_ | 1804150442788126720 |
|---|---|
| adam_text | TABLE OF CONTENTS I
TABLE OF CONTENTS
TABLE OF CONTENTS I
LIST OF ABBREVIATIONS IV
1 INTRODUCTION 1
2 SPRAY DRYING
OF PROTEINS 4
2.1 INTRODUCTION TO SPRAY DRYING 4
2.2 PROTEIN PROPERTIES 8
2.2.1 PRIMARY STRUCTURE 8
2.2.2 SECONDARY STRUCTURE 9
2.2.3 TERTIARY STRUCTURE 10
2.2.4 QUATERNARY STRUCTURE 11
2.2.5 ENZYMES 12
2.3 INACTIVATION OF ENZYMES DURING SPRAY DRYING 13
2.3.1 FEED ATOMIZATION 13
2.3.2 HEAT INACTIVATION 14
2.3.3 DEHYDRATION 15
2.4 PRINCIPLES OF PROTEIN STABILIZATION DURING SPRAY DRYING 16
2.4.1 SURFACTANTS STABILIZE PROTEINS DURING ATOMIZATION 16
2.4.2 INCREASING THE ENERGY BARRIER REDUCES HEAT SENSITIVITY 16
2.4.3 CARBOHYDRATES STABILIZE PROTEINS DURING DRYING 17
3 SINGLE DROPLET DRYING 20
3.1 HISTORY OF SINGLE DROPLET DRYING 20
3.2 EVAPORATION OF DROPLETS 22
3.2.1 EVAPORATION OF A SINGLE DROPLET 22
3.2.2 THEDMAW 23
3.2.3 DIFFUSION-CONTROLLED EVAPORATION 23
3.2.4 EVAPORATION OF DROPLETS CONTAINING DISSOLVED OR SUSPENDED SOLIDS
24
HTTP://D-NB.INFO/1037013840
II
TABLE OF CONTENTS
4 ACOUSTIC LEVITATION 32
4.1 INTRODUCTION TO ACOUSTIC LEVITATION 32
4.2 INTRODUCTION TO ACOUSTICS 35
4.3 FORCES IN AN ULTRASONIC LEVITATOR 37
4.3.1 THE BASIC PRINCIPLE - A STANDING ACOUSTIC WAVE 37
4.3.2 LEVITATION FORCES 38
4.3.3 ACOUSTIC STREAMING 39
4.3.4 INFLUENCE OF THE ACOUSTIC WAVE ON THE TEMPERATURE 41
4.4 THE DRYING OF SINGLE DROPLETS IN AN ACOUSTIC LEVITATOR 42
4.4.1 THE FIRST DRYING STAGE 42
4.4.2 THE SECOND DRYING STAGE 44
5 MATERIALS AND METHODS 46
5.1 MATERIALS 46
5.1.1 ENZYMES 46
5.1.2 EXCIPIENTS AND REAGENTS 51
5.1.3 OTHER MATERIALS 54
5.2 METHODS 55
5.2.1 ACOUSTIC LEVITATION 55
5.2.2 DROPLET/PARTICLE SIZE MEASUREMENT 58
5.2.3 DROPLET/PARTICLE SURFACE TEMPERATURE MEASUREMENT 58
5.2.4 DETERMINATION OF ENZYME INACTIVATION KINETICS 59
5.2.5 SPRAY DRYING 60
5.2.6 ENZYMATIC ASSAY OF L-GLUTAMIC DEHYDROGENASE 61
5.2.7 SCANNING ELECTRON MICROSCOPY 62
6 RESULTS AND DISCUSSION 63
6.1 L-LACTIC DEHYDROGENASE 63
6.1.1 ENZYMATIC ASSAY OF L-LACTIC DEHYDROGENASE (LDH) 63
6.1.2 SPRAY DRYING OF L-LACTIC DEHYDROGENASE 64
6.2 A-GLYCEROPHOSPHATE DEHYDROGENASE (GPDH) 66
6.2.1 ENZYMATIC ASSAY OF A-GLYCEROPHOSPHATE DEHYDROGENASE 66
6.2.2 WATERBATH STABILITY TESTS 67
6.2.3 LEVITATION OF GPDH SOLUTION DROPLETS 68
6.2.4 SPRAY DRYING OF GPDH 74
TABLE OF CONTENTS III
6.3 OCTOPINE DEHYDROGENASE 76
6.3.1 ENZYMATIC ASSAY OF OCTOPINE DEHYDROGENASE (ODH) 76
6.3.2 LEVITATION OF OCTOPINE DEHYDROGENASE SOLUTION DROPLETS 76
6.4 GLYCEROL DEHYDROGENASE 78
6.4.1 ENZYMATIC ASSAY OF GLYCEROL DEHYDROGENASE 78
6.4.2 LEVITATION OF GLYCEROL DEHYDROGENASE SOLUTION DROPLETS 78
6.5 A-CHYMOTRYPSIN 81
6.5.1 ENZYMATIC ASSAY OF A-CHYMOTRYPSIN 81
6.5.2 LEVITATION OF A-CHYMOTRYPSIN SOLUTION DROPLETS 81
6.6 PHYTASE 83
6.6.1 ENZYMATIC ASSAY OF PHYTASE 83
6.6.2 SPRAY DRYING OF PHYTASE 84
6.6.3 LEVITATION OF PHYTASE SOLUTION DROPLETS 85
6.7 A-AMYLASE 89
6.7.1 ENZYMATIC ASSAY OF A-AMYLASE 89
6.7.2 LEVITATION OF A-AMYLASE SOLUTION DROPLETS 90
6.8 SUMMARY OF PROTEINS DEEMED NOT SUITABLE 92
6.9 L-GLUTAMIC DEHYDROGENASE (GDH) 93
6.9.1 INFLUENCE OF THE DRYING AIR TEMPERATURE 94
6.9.2 INFLUENCE OF THE DRYING AIR RELATIVE HUMIDITY 102
6.9.3 EFFECT OF THE BUFFER CONCENTRATION 108
6.9.4 EFFECT OF GLUTAMATE CONCENTRATION 116
6.9.5 EFFECT OF TWEEN* 80 AS SURFACTANT 124
6.9.6 EFFECT OF TREHALOSE CONCENTRATION 131
6.9.7 EFFECTS OF SORBITOL CONCENTRATION 142
7 CONCLUSION 152
8 ZUSAMMENFASSUNG 157
9 APPENDIX 163
10 BIBLIOGRAPHY 174
|
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| dewey-full | 540 610 |
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| dewey-ones | 540 - Chemistry and allied sciences 610 - Medicine and health |
| dewey-raw | 540 610 |
| dewey-search | 540 610 |
| dewey-sort | 3540 |
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| discipline | Chemie / Pharmazie Medizin |
| format | Thesis Book |
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| spelling | Lorenzen, Elke Verfasser (DE-588)1035552051 aut Kinetics of protein damage during evaporative drying of levitated microdroplets von Elke Lorenzen 2013 VIII, 192 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2013 Levitation (DE-588)4120669-1 gnd rswk-swf Trocknung (DE-588)4124329-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Levitation (DE-588)4120669-1 s Trocknung (DE-588)4124329-8 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-45931 https://open.fau.de/handle/openfau/3311 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-45931 Resolving-System kostenfrei Volltext http://d-nb.info/1034425501/34 Langzeitarchivierung Nationalbibliothek DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026051834&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lorenzen, Elke Kinetics of protein damage during evaporative drying of levitated microdroplets Levitation (DE-588)4120669-1 gnd Trocknung (DE-588)4124329-8 gnd |
| subject_GND | (DE-588)4120669-1 (DE-588)4124329-8 (DE-588)4113937-9 |
| title | Kinetics of protein damage during evaporative drying of levitated microdroplets |
| title_auth | Kinetics of protein damage during evaporative drying of levitated microdroplets |
| title_exact_search | Kinetics of protein damage during evaporative drying of levitated microdroplets |
| title_full | Kinetics of protein damage during evaporative drying of levitated microdroplets von Elke Lorenzen |
| title_fullStr | Kinetics of protein damage during evaporative drying of levitated microdroplets von Elke Lorenzen |
| title_full_unstemmed | Kinetics of protein damage during evaporative drying of levitated microdroplets von Elke Lorenzen |
| title_short | Kinetics of protein damage during evaporative drying of levitated microdroplets |
| title_sort | kinetics of protein damage during evaporative drying of levitated microdroplets |
| topic | Levitation (DE-588)4120669-1 gnd Trocknung (DE-588)4124329-8 gnd |
| topic_facet | Levitation Trocknung Hochschulschrift |
| url | https://open.fau.de/handle/openfau/3311 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-45931 http://d-nb.info/1034425501/34 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026051834&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lorenzenelke kineticsofproteindamageduringevaporativedryingoflevitatedmicrodroplets |