Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130430-1130089-0-3 Inhaltsverzeichnis |
| Beschreibung: | VII, 165 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
1 INTRODUCTION 1
1.1 MESENCHYMAL STEM CELLS 1
1.2 MSC THERAPY 3
1.2.1 CLINICAL APPLICATION OF MSCS 3
1.2.2 MODE OF ACTION 5
1.3 MSC CULTURE 7
1.3.1 INFLUENCE OF CULTURE CONDITIONS 7
1.3.2 METABOLISM : 9
1.4 BIOREACTORS AND MSC BIOPROCESSING 10
1.4.1 BIOPROCESSING OF MSCS 10
1.4.2 MICROCARRIER-BASED EXPANSION OF MSCS IN BIOREACTORS 15
1.5 STIMULI-RESPONSIVE POLYMERS 18
1.5.1 INTRODUCTION 18
1.5.2 THERMORESPONSIVE POLYMERS FOR CELL CULTURE 21
2 OBJECTIVE OF THIS WORK 24
3 RESULTS 25
3.1 PROCESS DEVELOPMENT AND OPTIMIZATION 25
3.1.1 EVALUATION OF DIFFERENT MICROCARRIERS FOR MSC EXPANSION 25
3.1.2 PROCESS OPTIMIZATION: INOCULATION OF MSCS 30
3.2 MSC EXPANSION ON CYTODEX 1 MICROCARRIERS:
CHARACTERIZATION OF THE CULTIVATION PROCESS 33
3.2.1 CELL NUMBERS, POPULATION DOUBLINGS, AND DOUBLING TIME 34
3.2.2 PH VALUE AND OSMOLALITY 36
3.2.3 NUTRIENTS AND METABOLITES 38
3.2.4 CELL YIELD, VIABILITY, AND HARVEST EFFICIENCY 42
HTTP://D-NB.INFO/1038769590
IMAGE 2
TABLE OF CONTENTS
3.3 CELL CHARACTERIZATION AND COMPARATIVE ANALYSIS
OF MICROCARRIER- AND FLASK-EXPANDED MSCS 44
3.3.1 MORPHOLOGY 44
3.3.2 PHENOTYPE 45
3.3.3 DIFFERENTIATION OF MSCS INTO ADIPOGENIC, OSTEOGENIC,
AND CHONDROGENIC LINEAGES 48
3.3.4 IMMUNOSUPPRESSIVE CAPACITY OF MICROCARRIER- AND FLASK-EXPANDED
MSCS 53
3.3.5 CYTOKINE SECRETION OF MICROCARRIER- AND FLASK-EXPANDED MSCS 55
3.3.6 GENE EXPRESSION PATTERNS OF MICROCARRIER- AND FLASK-EXPANDED MSCS
57
3.4 SCALE-UP OF MSC EXPANSION ON MICROCARRIERS:
CULTIVATION UNDER REGULATED CONDITIONS 61
3.4.1 PHENOTYPE 61
3.4.2 IMMUNOSUPPRESSIVE CAPACITY OF MSCS 62
3.4.3 CYTOKINE SECRETION 63
3.4.4 GENE EXPRESSION ANALYSIS 65
3.5 CULTIVATION OF MSCS ON THERMORESPONSIVE SURFACES 66
3.5.1 CULTIVATION OF MSCS ON UPCELL* SURFACE 66
3.5.2 CULTIVATION OF MSCS ON NON-COMMERCIAL, THERMORESPONSIVE SURFACES
68
4 DISCUSSION 75
4.1 MSC CULTIVATION ON MICROCARRIERS:
PROCESS DEVELOPMENT, OPTIMIZATION, AND SCALE^UP 75
4.2 SOURCE AND DONOR VARIABILITY 80
4.3 INFLUENCE OF CULTURE CONDITIONS ON MSC CHARACTERISTICS AND FUNCTION
81
4.4 MSC CULTIVATION ON THERMORESPONSIVE SURFACES 87
5 SUMMARY AND OUTLOOK 92
6 ZUSAMMENFASSUNG UND AUSBLICK 94
7 MATERIAL AND METHODS 97
IMAGE 3
TABLE OF CONTENTS
7.1 MATERIAL 97
7.1.1 MICROCARRIER 98
7.1.2 ANTIBODIES 99
7.1.3 KITS 100
7.1.4 REAGENTS 100
7.1.5 SOFTWARE 102
7.2 METHODS 103
7.2.1 ISOLATION OF MSCS FROM UMBILICAL CORD AND AMNIOTIC MEMBRANE 103
7.2.2 CELL CULTURE AND EXPANSION 103
7.2.3 MICROSCOPIC ANALYSIS OF MSCS ON MICROCARRIERS 107
7.2.4 ALAMARBLUE ASSAY 108
7.2.5 MEDIUM ANALYSIS 108
7.2.6 CELL CHARACTERIZATION AFTER EXPANSION 109
7.2.7 STATISTICAL ANALYSIS 115
8 REFERENCES 116
9 SUPPLEMENTS 139
|
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| spelling | Hupfeld, Julia Verfasser aut Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors Julia Hupfeld 2013 VII, 165 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2013 Bioreaktor (DE-588)4006780-4 gnd rswk-swf Mesenchymzelle (DE-588)4278308-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Mesenchymzelle (DE-588)4278308-2 s Bioreaktor (DE-588)4006780-4 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:91-diss-20130430-1130089-0-3 http://mediatum.ub.tum.de/node?id=1130089 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:91-diss-20130430-1130089-0-3 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=026018309&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hupfeld, Julia Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors Bioreaktor (DE-588)4006780-4 gnd Mesenchymzelle (DE-588)4278308-2 gnd |
| subject_GND | (DE-588)4006780-4 (DE-588)4278308-2 (DE-588)4113937-9 |
| title | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors |
| title_auth | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors |
| title_exact_search | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors |
| title_full | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors Julia Hupfeld |
| title_fullStr | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors Julia Hupfeld |
| title_full_unstemmed | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors Julia Hupfeld |
| title_short | Expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors |
| title_sort | expansion and modulation of mesenchymal stem cells on biomaterials in bioreactors |
| topic | Bioreaktor (DE-588)4006780-4 gnd Mesenchymzelle (DE-588)4278308-2 gnd |
| topic_facet | Bioreaktor Mesenchymzelle Hochschulschrift |
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