Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.):
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| Format: | Thesis Book |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | VII, 137 S. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) |c angefertigt von Anja Thalhammer, geb. Braig |
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| adam_text | IMAGE 1
TABLE OF CONTENTS III
TABLE O F CONTENTS
ZUSAMMENFASSUNG I
SUMMARY II
TABLE O F CONTENTS ILL
LIST OF FIGURES V
LIST OF ABBREVIATIONS VII
1 INTRODUCTION 1
1.1 PLANTS IN LOW TEMPERATURES 1
1.1.1 IMPACT O F LOW T E M P E R A T U R E S O N PLANTS 1
1.1.2 CELLULAR CONSEQUENCES A N D D A M A G E RESULTING FROM FREEZING 1
1.1.3 COLD ACCLIMATION 2
1.1.4 REGULATION OF COLD ACCLIMATION B Y T H E CBF P A T H W A Y 4
1.1.5 SENSING O F L O W TEMPERATURES, SIGNALLING A N D U P S T R E A M
REGULATION 5
1.1.6 CONSEQUENCES OF L O W T E M P E R A T U R E S O N T H E
CHLOROPLAST 6
1.2 LEA PROTEINS 6
1.2.1 LEA PROTEINS A R E A B U N D A N T IN P L A N T A N D ANIMAL
KINGDOMS 6
1.2.2 EXPRESSION A N D ACCUMULATION O F LEA G E N E S / P R O T E I N S
7
1.2.3 REGULATION O F LEA GENE EXPRESSION 8
1.2.4 POST TRANSLATIONAL MODIFICATIONS O F LEA PROTEINS 9
1.2.5 COMMON CHARACTERISTICS OF LEA PROTEINS 9
1.2.6 STRUCTURAL PROPERTIES O F LEA PROTEINS 9
1.2.7 FUNCTIONS O F LEA PROTEINS 10
1.3 MANY COR PROTEINS ARE LEA PROTEINS 13
1.4 CHARACTERIZATION OF ARABIDOPSIS COR15A AND COR15B 1 4
1.5 A. THALIANA AS MODEL ORGANISM 15
2 AIMS OF MY THESIS 16
3 MATERIALS AND METHODS 17
3.1 MATERIALS 17
3.1.1 CONSUMABLES 1 7
3.1.2 EQUIPMENT 1 7
3.1.3 KITS 19
3.1.3 ENZYMES A N D PROTEINS 19
3.1.4 MEDIA 19
3.1.5 REAGENTS A N D CHEMICALS 2 0
3.1.6 LIPIDS 2 1
3.1.7 ANTIBODIES 2 1
3.1.8 VECTORS 2 1
3.1.9 PRIMERS 2 1
3.1.10 BACTERIA 22
3.1.11 SOFTWARE 2 3
3.1.12 ONLINE TOOLS 2 3
3.2 METHODS 2 4
3.2.1 PLANT G R O W T H A N D T R E A T M E N T S 2 4
3.2.2 PHYSIOLOGICAL M E T H O D S 2 5
3.2.3 CELL BIOLOGICAL M E T H O D S 2 8
3.2.4 MOLECULAR BIOLOGICAL M E T H O D S 2 8
3.2.5 BIOCHEMICAL M E T H O D S 3 4
3.2.6 BIOPHYSICAL M E T H O D S 3 7
3.2.7 IN SILICO M E T H O D S 39
3.2.8 STATISTICAL ANALYSIS 4 0
HTTP://D-NB.INFO/1036563774
IMAGE 2
TABLE OF CONTENTS
IV
4 RESULTS 4 1
4.1 IN SILICO ANALYSIS OF FIVE SELECTED LEA PROTEINS AND THEIR ENCODING
GENES 4 1 4.1.1 SIMILARITIES B E T W E E N T H E SELECTED LEA GENES A N
D PROTEINS 4 1
4.1.2 DISORDER A N D SECONDARY STRUCTURE PREDICTION 4 2
4.1.3 EXPRESSION A N D CO-EXPRESSION ANALYSIS 4 3
4.1.4 EVOLUTION OF THE SELECTED LEA GENES 4 6
4.2 CLONING AND EXPRESSION O F THE SELECTED LEA PROTEINS 4 8
4.2.1 GATEWAY-CLONING A N D SUBCLONING 4 8
4.2.2 EXPRESSION A N D PURIFICATION O F RECOMBINANT LEA PROTEINS IN E.
COLI 4 8
4.2.3 GENERATION, SCREENING A N D CHARACTERIZATION O F HOMOZYGOUS C0R15
RNAI SILENCING A THALIANA LINES 4 9
4.2.4 CHARACTERIZATION O F C0R15A A N D C0R15B OVEREXPRESSING LINES 50
4.3 EVALUATION OF TOTAL COR15 AMOUNTS IN WILDTYPE LEAVES 5 1
4.4 LOCALIZATION OF CANDIDATE LEA PROTEINS 5 1
4.5 EFFECTS OF RNAI SILENCING AND OVEREXPRESSION OF COR15 GENES ON A.
THALIANA PLANTS 52
4.5.1 CONTENT O F COMPATIBLE SOLUTES IN COR15 RNAI SILENCING A N D
OVEREXPRESSING PLANTS 52
4.5.2 PLANT GROWTH, MORPHOLOGY A N D DEVELOPMENT O F COR15 RNAI
SILENCING A N D OVEREXPRESSING PLANTS 5 5
4.5.3 EFFECTS O F COR15 RNAI SILENCING A N D OVEREXPRESSING O N A.
THALIANA FREEZING TOLERANCE 6 3
4.5.4 EFFECTS O F COR15 RNAI SILENCING A N D OVEREXPRESSION ON T H E
ACTIVITY A N D
INTEGRITY O F SELECTED CHLOROPLAST ENZYMES DURING FREEZING 6 6
4.6 CHARACTERIZATION O F RECOMBINANT LEA PROTEINS 7 1
4.6.1 STRUCTURAL CHARACTERIZATION O F RECOMBINANT LEA PROTEINS IN
DIFFERENT
CONDITIONS O F W A T E R AVAILABILITY IN T H E PRESENCE A N D ABSENCE OF
ARTIFICIAL M E M B R A N E S 7 1
4.6.2 THE FUNCTIONAL ROLE O F LEA PROTEINS IN T H E STABILIZATION O F
ARTIFICIAL MEMBRANES.... 7 6 5 DISCUSSION 7 9
5.1 EXPRESSION AND REGULATION OF THE SELECTED LEA GENES 7 9
5.2 EVOLUTIONARY RELATIONSHIPS BETWEEN THE CANDIDATE LEA GENES 8 1
5.3 SUBCELLULAR LOCALIZATION O F THE SELECTED LEA PROTEINS 82
5.4 CHARACTERIZATION OF COR15A AND COR15B 82
5.4.1 ALTERING COR15A A N D COR1SB GENE EXPRESSION 82
5.4.2 STRUCTURAL PROPERTIES O F COR15A A N D COR15B 9 1
5.4.3 MOLECULAR FUNCTION O F COR15A A N D COR15B 9 4
5.4.4 ABUNDANCE O F COR15 PROTEINS IN T H E PLANT 9 9
5.5 STRUCTURE-FUNCTION RELATIONSHIP O F LEAH AND LEA25 100
5.6 CONCLUDING REMARKS - THE ROLE OF THE SELECTED LEA PROTEINS -
IMPLICATIONS FROM THE INTEGRATION OF IN VIVO, IN VITRO AND IN SILICO
RESULTS 102
6 OUTLOOK 106
7 REFERENCES 107
APPENDIX 130
ACKNOWLEDGEMENTS 134
LIST OF PUBLICATIONS AND PRESENTATIONS 135
EIDESSTATTLICHE ERKLARUNG 137
|
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| author | Thalhammer, Anja |
| author_facet | Thalhammer, Anja |
| author_role | aut |
| author_sort | Thalhammer, Anja |
| author_variant | a t at |
| building | Verbundindex |
| bvnumber | BV041000961 |
| ctrlnum | (OCoLC)854710521 (DE-599)GBV743896394 |
| dewey-full | 572.69362364 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.69362364 |
| dewey-search | 572.69362364 |
| dewey-sort | 3572.69362364 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| indexdate | 2024-07-10T00:37:10Z |
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| language | English |
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| physical | VII, 137 S. Ill., graph. Darst. |
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| spelling | Thalhammer, Anja Verfasser aut Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) angefertigt von Anja Thalhammer, geb. Braig 2012 VII, 137 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2012 Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Frostresistenz (DE-588)4136406-5 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Frostresistenz (DE-588)4136406-5 s Genexpression (DE-588)4020136-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025978562&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Thalhammer, Anja Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) Ackerschmalwand (DE-588)4141299-0 gnd Frostresistenz (DE-588)4136406-5 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4141299-0 (DE-588)4136406-5 (DE-588)4020136-3 (DE-588)4113937-9 |
| title | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) |
| title_auth | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) |
| title_exact_search | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) |
| title_full | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) angefertigt von Anja Thalhammer, geb. Braig |
| title_fullStr | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) angefertigt von Anja Thalhammer, geb. Braig |
| title_full_unstemmed | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) angefertigt von Anja Thalhammer, geb. Braig |
| title_short | Physiological, functional and structural characterization of five closely related COR/LEA (COld regulated/late embryogenesis abundant) proteins from arabidopsis thaliana (L.) |
| title_sort | physiological functional and structural characterization of five closely related cor lea cold regulated late embryogenesis abundant proteins from arabidopsis thaliana l |
| topic | Ackerschmalwand (DE-588)4141299-0 gnd Frostresistenz (DE-588)4136406-5 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Ackerschmalwand Frostresistenz Genexpression Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025978562&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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