Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. und engl. Sprache |
| Beschreibung: | 147 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV040996228 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 130514s2012 ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)914847097 | ||
| 035 | |a (DE-599)HBZHT017515220 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-188 |a DE-83 | ||
| 100 | 1 | |a Dejanović, Borislav |d 1983- |e Verfasser |0 (DE-588)1029912785 |4 aut | |
| 245 | 1 | 0 | |a Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis |c vorgelegt von: Borislav Dejanović |
| 264 | 1 | |c 2012 | |
| 300 | |a 147 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. und engl. Sprache | ||
| 502 | |a Köln, Univ., Diss., 2012 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025973921&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-025973921 | ||
Datensatz im Suchindex
| _version_ | 1804150321175330816 |
|---|---|
| adam_text | Titel: Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteos
Autor: Dejanović, Borislav
Jahr: 2012
Table of contents
1 Introduction_____________________________________________________3
1.1 Synaptic transmission_____________________________________________3
1.2 Synaptic inhibition________________________________________________. 4
1.3 Structure and function of glycine and GABA receptors__________________ 5
1.4 Postsynaptic density______________________________________________6
1.5 Gephyrin________________________________________________________7
1.5.1 Clustering of inhibitory receptors at synapses________________________7
1.5.2 Synaptic plasticity at inhibitory synapses___________________________10
1.5.3 Protein and gene structure of gephyrin____________________________11
1.5.4 Clustering of gephyrin_______________________________________13
1.5.5 Gephyrin interacting proteins___________________________________14
1.5.6 Post-translational modification of gephyrin_________________________15
1.6 Palmitoylation__________________________________________________ 16
1.6.1 Palmitoyl acyl transferases____________________________________18
1.6.2 Protein depalmitoytation______________________________________21
1.7 S-nitrosylation__________________________________________________22
1.8 Gephyrin in disease______________________________________________23
1.9 Aims of study ___________________________________________25
2 Results________________________________________________________26
2.1 Gephyrin is membrane associated In vivo____________________________26
2.2 Gephyrin is paimitoylated_________________________________________29
2.3 Gephyrin palmitoylation triggers membrane association________________32
2.4 Gephyrin palmitoylation is essential for proper synapse targeting and
GABAergic synapse assembly______________________________________33
2.5 Gephyrin palmitoylation is regulated by synaptic strength_______________37
2.6 Palmitoylation influences gephyrin stability and regulates the amount of
synaptic gephyrin_______________________________________________38
2.6.1 Palmitoylation does not modify biophysical parameters of gephyrin but influences
proteolytic degradation_______________________________________41
2.7 Identification of gephyrin palmitoylatlng enzymes_____________________44
2.7.1 Gephyrin can be palmitoylated by several palmitoyl transferases in non-neuronal
cells___________________________________________________44
2.7.2 Presence of DHHC proteins does not modify gephyrin blob formation_______46
2.7.3 DHHC12 and 16 modulate gephyrin cluster size and intensity in cultured
hippocampal neurons________________________________________48
2.7.4 Distinct subcellular localization of DHHC12 and 16 in neuronal cells________50
2.7.5 Gephyrin co-localizes with DHHC12 in neurites______________________52
2.7.6 Gephyrin forms complexes with DHHC12 and 16_____________________54
2.7.7 Inactive palmitoyl transferase mutants block gephyrin palmitoylation in
heterologous cells__________________________________________55
2.8 Identification of palmitoylated cysteine residues_______________________56
2.9 Age-related gephyrin palmitoylation_________________________________60
2.10 Regulation of gephyrin depalmitoylation_____________________________60
2.10.1 Gephyrin is nitrosylated in vivo_________________________________61
2.10.2 Nitrosylation competes with palmitoylation on gephyrin________________62
2.10.3 Gephyrin interacts with nNOS_________________________________64
2.10.4 Quantification of palmitoylated protein in vivo_______________________65
2.1 Heterozygous gephyrin truncation in a patient with idiopathic generalized
epilepsy________________________________________________________67
3 Discussion_____________________________________________________76
3.1 Gephyrin is membrane-associated_________________________________76
3.2 Gephyrin palmitoylation__________________________________________79
3.2.1 Gephyrin palmitoylation regulates its localization at inhibitory synapses_____82
3.2.2 Gephyrin palmitoylation is regulated by synaptic activity________________84
3.2.3 Palmitoylation-dependent gephyrin turnover________________________85
3.2.4 DHHC12 and DHHC16 are gephyrin palmitoylating transferases__________88
3.3 Gephyrin nitrosylation competes with palmitoylation___________________91
3.4 Gephyrin palmitoylation sites______________________________________95
3.5 Age-dependent gephyrin palmitoylation______________________________96
3.6 Heterozygous gephyrin truncation in a patient with idiopathic generalized
epilepsy________________________________________________________97
4 Materials and methods_________________________________________102
4.1 Materials______________________________________________________ 102
4.2 Molecular biological methods____________________________________104
4.3 General methods_______________________________________________107
4.4 Protein expression, cell transection and immunostaining______________108
4.5 Biochemical methods___________________________________________111
4.6 Biophysical methods____________________________________________120
5 Appendix_____________________________________________________121
6 References____________________________________________________124
7 Acknowledgment______________________________________________144
|
| any_adam_object | 1 |
| author | Dejanović, Borislav 1983- |
| author_GND | (DE-588)1029912785 |
| author_facet | Dejanović, Borislav 1983- |
| author_role | aut |
| author_sort | Dejanović, Borislav 1983- |
| author_variant | b d bd |
| building | Verbundindex |
| bvnumber | BV040996228 |
| ctrlnum | (OCoLC)914847097 (DE-599)HBZHT017515220 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01172nam a2200289 c 4500</leader><controlfield tag="001">BV040996228</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">130514s2012 ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)914847097</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)HBZHT017515220</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-188</subfield><subfield code="a">DE-83</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Dejanović, Borislav</subfield><subfield code="d">1983-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1029912785</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis</subfield><subfield code="c">vorgelegt von: Borislav Dejanović</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">147 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Zsfassung in dt. und engl. Sprache</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Köln, Univ., Diss., 2012</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025973921&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-025973921</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040996228 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:37:04Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025973921 |
| oclc_num | 914847097 |
| open_access_boolean | |
| owner | DE-188 DE-83 |
| owner_facet | DE-188 DE-83 |
| physical | 147 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spelling | Dejanović, Borislav 1983- Verfasser (DE-588)1029912785 aut Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis vorgelegt von: Borislav Dejanović 2012 147 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. und engl. Sprache Köln, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025973921&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dejanović, Borislav 1983- Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis |
| subject_GND | (DE-588)4113937-9 |
| title | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis |
| title_auth | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis |
| title_exact_search | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis |
| title_full | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis vorgelegt von: Borislav Dejanović |
| title_fullStr | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis vorgelegt von: Borislav Dejanović |
| title_full_unstemmed | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis vorgelegt von: Borislav Dejanović |
| title_short | Gephyrin palmitoylation and oligomerization in synaptic plasticity, membrane recruitment and proteostasis |
| title_sort | gephyrin palmitoylation and oligomerization in synaptic plasticity membrane recruitment and proteostasis |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025973921&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT dejanovicborislav gephyrinpalmitoylationandoligomerizationinsynapticplasticitymembranerecruitmentandproteostasis |