Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 223 S. Ill., graph. Darst. |
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| 100 | 1 | |a Malinova, Irina |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana |c von Irina Malinova |
| 264 | 1 | |c 2012 | |
| 300 | |a 223 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
ABBREVIATIONS 1
ABSTRACT 4
ZUSAMMENFASSUNG 5
DECLARATION ON CONTRIBUTION TO MANUSCRIPTS 6
STRUCTURE OF THE THESIS 8
CHAPTER 1. GENERAL INTRODUCTION 9
1.1 VARIETY OF CARBOHYDRATES IN HIGHER PLANTS 9
1.2 STRUCTURE AND COMPOSITION OF STARCH 10
1.3 SYNTHESIS OF TRANSITORY STARCH IN THE CHLOROPLASTS 11
1.4 DEGRADATION OF TRANSITORY STARCH IN THE CHLOROPLASTS 15
1.5 FURTHER STARCH DEGRADATION PROCESSES IN THE CYTOSOL 18
1.6 WATER-SOLUBLE HETEROGLYCANS, THEIR COMPOSITION AND FUNCTIONS 19
1.6.1 EXTRACTION AND CELLULAR LOCALIZATION OF WATER-SOLUBLE
HETEROGLYCANS 19
1.6.2 CYTOSOLIC HETEROGLYCANS ARE PHYSIOLOGICAL SUBSTRATE GLUCAN
TRANSFERASES 21
1.7 AIMS OF THE THESIS 23
STUDIES PERFORMED DURING THE PHD 24
CHAPTER 2. IMPACT OF GLUCOSE 1-PHOSPHATE TRANSPORT ON STARCH METABOLISM
IN A. 24
THALIANA PROTOPLASTS (PUBLICATION 1)
2.1 UPTAKE OF GLUCOSE 1-PHOSPHATE BY A. THALIANA PROTOPLASTS 25
2.2 FURTHER METABOLISM OF GLUCOSE 1-PHOSPHATE IN MESOPHYLL PROTOPLASTS
25
2.3 ANALYSIS OF ADP-GLUCOSE DEPENDENT UTILISATION OF GLUCOSE 1
-PHOSPHATE 26
2.4 UPTAKE OF GLUCOSE 1-PHOSPHATE BY P G M L MUTANTS 27
CHAPTER 3. IMPORTANCE OF THE PLASTIDIAL PHOSPHORYLASE IN LEAF STARCH
METABOLISM OF 28
ARABIDOPSIS THALIANA (MANUSCRIPT 1)
3.1 LOSS OF PLASTIDIAL PHOSPHORYLASE IN THE M E X L AND DPE2-1
BACKGROUNDS CAUSE 29
SEVERE PHENOTYPE
HTTP://D-NB.INFO/1036338630
IMAGE 2
3.2 LEAVES HETEROGENEITY IN ROSETTES OF DOUBLE MUTANTS 34
3.3 GROWING OF DOUBLE MUTANTS UNDER CONTINUOUS ILLUMINATION IMPROVE
DEFECTIVE 38
PHENOTYPES
3.4. DISCUSSION 40
CHAPTER 4. FUNCTIONAL ANALYSIS OF CYTOSOLIC PHOSPHOGLUCOMUTASES IN
ARABIDOPSIS 43
THALIANA PLANTS (UNPUBLISHED)
4.1 ANALYSIS OF SINGLE CPGM MUTANTS 44
4.2 ANALYSIS OF DOUBLE MUTANTS DEFICIENT IN ONE CYTOSOLIC PGM AND
PLASTIDIAL PGM1 46
4.3 ANALYSIS OF TRANSGENIC PLANTS WITH SIGNIFICANTLY REDUCED ACTIVITIES
OF BOTH CYTOSOLIC 49
PGMS
4.3.1 IMPACT OF REDUCED CPGM ACTIVITIES ON PHOTOAUTOTROPHIC TISSUES 49
4.3.2 IMPACT OF REDUCED CPGM ACTIVITIES ON HETEROTROPHIC TISSUES 53
4.4 ANALYSIS OF TRANSGENIC PLANTS WITH REDUCED ACTIVITY OF BOTH
PLASTIDIAL AND CYTOSOLIC 54
PHOSPHOGLUCOMUTASES
4.5 DISCUSSION 58
CHAPTER 5. PHOTOSYNTHESIS AND STARCH DEPENDENT CARBON FLUXES TO THE
HETEROGLYCANS 61
IN A. THALIANA PROTOPLASTS (PUBLICATION 2, MANUSCRIPT 2)
5.1 ANALYSIS OF STARCH AND HETEROGLYCANS FROM THE PROTOPLASTS INCUBATED
UNDER 62
CONTINUOUS ILLUMINATION
5.2 CARBON FLUXES TO THE HETEROGLYCANS DURING LIGHT / DARK TRANSITION 63
5.3 CARBON FLUX TO THE HETEROGLYCANS UNDER CONTINUOUS ILLUMINATION 64
CHAPTER 6. ANALYSIS OF STARCH AND STARCH-RELATED HETEROGLYCANS IN
AUTROTROPHIC AND 65
HETEROTROPHIC TISSUES (PUBLICATIONS 3, 4)
6.1 ANALYSIS OF STARCH IN LEAVES AND ROOTS 65
6.2 HETEROGLYCANS IN LEAVES AND ROOTS 67
6.3 ACTIVITY OF STARCH RELATED ENZYMES (DPE2 AND THE PHOSPHORYLASES) 67
CHAPTER 7. ANALYSIS OF STARCH RELATED METABOLISM IN ARABIDOPSIS THALIANA
ACCESSIONS 69
(PARTIALLY IN PUBLICATION 2)
IMAGE 3
7.1 PHENOTYPES OF DIFFERENT A. THALIANA ACCESSIONS 70
7.2 STARCH CONTENT IN LEAVES OF A. THALIANA ACCESSIONS 71
7.3 ANALYSIS OF SHG L FROM LEAVES OF DIFFERENT ACCESSIONS 72
7.4 ANALYSIS OF PHOSPHORYLASES AND DPE2 ACTIVITY IN LEAVES OF VARIOUS
ACCESSIONS 73
7.5 DIURNAL CHANGES OF STARCH CONTENT, DPE2 AND PHOSPHORYLASES
ACTIVITIES IN BSCH-2 76
ACCESSION
7.6 PHOSPHOGLUCOMUTASES ACTIVITIES IN LEAVES OF VARIOUS ACCESSIONS 76
7.7 DISCUSSION 77
ORIGINAL PUBLICATIONS AND SUBMITTED MANUSCRIPTS 79
PUBLICATION 1. GLUCOSE-1-PHOSPHATE TRANSPORT INTO PROTOPLASTS AND
CHLOROPLASTS FROM 79
LEAVES OF ARABIDOPSIS
PUBLICATION 2. CYTOSOLIC HETEROGLYCANS IN PHOTOAUTOTROPHIC AND IN
HETEROTROPHIC PLANT 92
CELLS
PUBLICATION 3. STARCH-RELATED CYTOSOLIC HETEROGLYCANS IN ROOTS FROM
ARABIDOPSIS 100
THALIANA
PUBLICATION 4. STARCH-RELATED CARBON FLUXES IN ROOTS AND LEAVES OF
ARABIDOPSIS 110
THALIANA
MANUSCRIPT 1. DOUBLE KNOCK-OUT MUTANTS OF A. THALIANA REVEAL AN
ESSENTIAL 114
BIOCHEMICAL FUNCTION OF THE PLASTIDIAL PHOSPHORYLASE ISOZYME (PHS1) IN
TRANSITORY
STARCH DEGRADATION
MANUSCRIPT 2. CARBON TRANSITIONS FROM EITHER CALVIN CYCLE OR TRANSITORY
STARCH TO 150
HETEROGLYCANS AS REVEALED BY 14 C-LABELLING EXPERIMENTS USING
PROTOPLASTS FROM
ARABIDOPSIS
CHAPTER 8. SUMMARY AND GENERAL DISCUSSION 193
8.1 THE IMPACT OF TRANSPORTED GLUCOSE 1-PHOSPHATE FOR STARCH
BIOSYNTHESIS 193
8.2 THE ESSENTIAL FUNCTION OF PLASTIDIAL PHOSPHORYLASE FOR STARCH
METABOLISM IN 193
ARABIDOPSIS THALIANA
8.3 THE IMPACT OF CYTOSOLIC PGMS IN CARBOHYDRATES PARTITIONING 195
IMAGE 4
8.4 THE ROLE OF HETEROGLYCANS AND HETEROGLYCAN RELATED ENZYMES IN STARCH
196
DEGRADATION
REFERENCES 199
SUPPLEMENTAL MATERIAL 218
ACKNOWLEDGMENTS 222
|
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| author | Malinova, Irina |
| author_facet | Malinova, Irina |
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| dewey-search | 572.56642364 |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| spelling | Malinova, Irina Verfasser aut Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana von Irina Malinova 2012 223 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2012 Glucanotransferase 4-alpha- (DE-588)4366148-8 gnd rswk-swf Stärkestoffwechsel (DE-588)4182797-1 gnd rswk-swf Transgene Pflanzen (DE-588)4311513-5 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Stärkestoffwechsel (DE-588)4182797-1 s Ackerschmalwand (DE-588)4141299-0 s Transgene Pflanzen (DE-588)4311513-5 s Glucanotransferase 4-alpha- (DE-588)4366148-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025958651&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Malinova, Irina Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana Glucanotransferase 4-alpha- (DE-588)4366148-8 gnd Stärkestoffwechsel (DE-588)4182797-1 gnd Transgene Pflanzen (DE-588)4311513-5 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| subject_GND | (DE-588)4366148-8 (DE-588)4182797-1 (DE-588)4311513-5 (DE-588)4141299-0 (DE-588)4113937-9 |
| title | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana |
| title_auth | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana |
| title_exact_search | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana |
| title_full | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana von Irina Malinova |
| title_fullStr | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana von Irina Malinova |
| title_full_unstemmed | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana von Irina Malinova |
| title_short | Analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn-over in Arabidopsis thaliana |
| title_sort | analysis of starch metabolism in connection with altered enzyme activities related to central carbon turn over in arabidopsis thaliana |
| topic | Glucanotransferase 4-alpha- (DE-588)4366148-8 gnd Stärkestoffwechsel (DE-588)4182797-1 gnd Transgene Pflanzen (DE-588)4311513-5 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Glucanotransferase 4-alpha- Stärkestoffwechsel Transgene Pflanzen Ackerschmalwand Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025958651&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT malinovairina analysisofstarchmetabolisminconnectionwithalteredenzymeactivitiesrelatedtocentralcarbonturnoverinarabidopsisthaliana |