Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1):
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch und Buch Verl.
2013
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Entwicklung eines Impstoffes gegen das pandemische Influenzavirus A (09-H1N1) basierend auf einem equinen Herpesvirus Typ 1 (EHV-1)-Vektor |
| Beschreibung: | VIII, 84 S. Ill., graph. Darst. |
| ISBN: | 9783863872991 |
Internformat
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| 245 | 1 | 0 | |a Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) |c vorgelegt von Abdelrahman Fekry Abdelrahman Said |
| 246 | 1 | 3 | |a Entwicklung eines Impstoffes gegen das pandemische Influenzavirus A (09-H1N1) basierend auf einem equinen Herpesvirus Typ 1 (EHV-1)-Vektor |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2013 | |
| 300 | |a VIII, 84 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Entwicklung eines Impstoffes gegen das pandemische Influenzavirus A (09-H1N1) basierend auf einem equinen Herpesvirus Typ 1 (EHV-1)-Vektor | ||
| 502 | |a Berlin, Freie Univ., Diss., 2013 | ||
| 650 | 7 | |a Equid herpesvirus 1 |2 cabt | |
| 650 | 7 | |a vectors |2 cabt | |
| 650 | 7 | |a vaccines |2 cabt | |
| 650 | 7 | |a Influenza A virus |2 cabt | |
| 650 | 7 | |a recombination |2 cabt | |
| 650 | 7 | |a western blotting |2 cabt | |
| 650 | 7 | |a immunofluorescence |2 cabt | |
| 650 | 7 | |a animal experiments |2 cabt | |
| 650 | 0 | 7 | |a Impfstoff |0 (DE-588)4026655-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Equines Herpesvirus 1 |0 (DE-588)4320759-5 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804150293479292928 |
|---|---|
| adam_text | IMAGE 1
LIST OF CONTENTS
LIST OF CONTENTS
LIST OF CONTENTS I
LIST OF FIGURES IV
LIST OF TABLES V
ABBREVIATION VI
ZUSAMMENFASSUNG 1
SUMMARY 3
CHAPTER 1: GENERAL INTRODUCTION 5
1.1. VACCINE HISTORY 5
1.2. TYPES OF VACCINES 5
1.2.1. LIVE ATTENUATED VACCINES 6
1.2.2. KILLED OR INACTIVATED VACCINES 6
1.2.3. SUBUNIT VACCINES 6
1.2.4. GENE-DELETED VACCINES 7
1.2.5. DNA VACCINES 7
1.2.6. RECOMBINANT VECTOR VACCINES 9
1.3. GENERAL CHARACTERISTICS OF EQUINE HERPESVIRUS TYPE L(EHV-L) 10
1.3.1. CLASSIFICATION AND STRUCTURE OFEHV-1 10
1.3.2. PATHOGENESIS, REPLICATION AND LATENCY OFEHV-1 11
1.3.3. CLONING OF HERPESVIRUSES AS BACTERIAL ARTIFICIAL CHROMOSOME (BAC)
12
1.3.3.A. BACS IN VIROLOGY 12
I.3.3.B. APPLICATION OF BAC TECHNOLOGY TO HERPESVIRUSES 13
I.3.3.C. POTENTIAL OF EQUINE HERPESVIRUS TYPE 1 AS A VECTOR FOR
IMMUNIZATION 14
1.4. PANDEMIC INFLUENZA A VIRUS 15
1.4.1. BACKGROUND AND HISTORY 15
1.4.2. STRUCTURE OF INFLUENZA A VIRUS 17
1.4.3. HISTORY OF PANDEMIC INFLUENZA A(H1N1) 2009 REASSORTMENT 17
1.4.4. CONTROL AND PREVENTION OF PANDEMIC INFLUENZA 18
1.5. OUTLINE OF THE THESIS 19
REFERENCE 20
CHAPTER 2: AN EQUINE HERPESVIRUS 1 (EHV-1) VECTORED HI VACCINE PROTECTS
AGAINST
CHALLENGE WITH SWINE-ORIGIN INFLUENZA VIRUS H1N1 29
2.1. SUMMARY 29
HTTP://D-NB.INFO/1034049046
IMAGE 2
LIST OF CONTENTS
2.2. INTRODUCTION 29
2.3. MATERIALS AND METHODS 31
2.3.1. VIRUSES AND CELLS 31
2.3.2. BAC CLONING AND MUTAGENESIS 31
2.3.3. RECONSTITUTION OF INFECTIOUS VIRUS FROM BAC DNA AND VIRUS GROWTH
ASSAYS 34
2.3.4. INDIRECT IMMUNOFLUORESCENCE ASSAYS (IFA) AND WESTERN BLOTTING 35
2.3.5. ANIMAL EXPERIMENTS 36
2.4. RESULTS 37
2.4.1. GENERATION OF A VECTORED RACH VACCINE EXPRESSING S-OIV HA (RH HL)
37
2.4.2. IN VITRO CHARACTERIZATION OF RH_HL 38
2.4.3 SEROLOGICAL RESPONSES INDUCED IN MICE IMMUNIZED WITH RH_HL 40
2.4.4. PROTECTIVE EFFICACY OF EHVI-EXPRESSED HI IN MICE 41
2.5. DISCUSSION 43
ACKNOWLEDGMENTS 47
REFERENCES 48
CHAPTER 3: RECOMBINANT EQUINE HERPESVIRUS 1 (EHV-1) VACCINE PROTECTS
PIGS AGAINST
CHALLENGE WITH INFLUENZA A(HLNL)PMD09 54
3.1. SUMMARY 54
3.2. INTRODUCTION 54
3.3. MATERIALS AND METHODS 56
3.3.1. VIRUSES AND CELLS 56
3.3.2. ANIMALS 57
3.3.3. EXPERIMENTAL DESIGN 57
3.3.4. CLINICAL RECORDS AND SAMPLING 57
3.3.5. HEMAGGLUTINATION INHIBITION (HI) ASSAY 58
3.3.6. QUANTITATIVE REVERSE TRANSCRIPTASE REAL TIME PCR (QRT-PCR) 58
3.4. RESULTS 59
3.4.1. SEROLOGICAL RESPONSES ARE INDUCED IN RH_HL-IMMUNIZED PIGS PRIOR
TO
EXPERIMENTAL CHALLENGE 59
3.4.2. IMMUNIZATION OF PIGS WITH RH_HL RESULTS IN PROTECTION AGAINST
EXPERIMENTAL
CHALLENGE INFECTION 60
3.4.3. IMMUNIZED ANIMALS DEVELOPED A SPECIFIC HUMORAL ANAMNESTIC
RESPONSE THAT
INTERFERE WITH REPLICATION OF CHALLENGE VIRUS 61
IMAGE 3
LIST OF CONTENTS
3.4.4. VACCINATION REDUCED VIRAL SHEDDING OF CHALLENGE VIRUS 63
3.5 DISCUSSION 63
ACKNOWLEDGMENTS 66
REFERENCES 66
CHAPTER 4: GENERAL DISCUSSION 72
4.1. PANDEMIC INFLUENZA VIRUS 72
4.2. IMMUNE RESPONSES TO INFLUENZA VIRUS 73
4.3. INFLUENZA VIRUS VACCINES 73
4.4. DEVELOPMENT OF NEW VACCINES 74
4.5. DEVELOPMENT OF EHV-1 AS VECTOR EXPRESSING THE HLOF PANDEMIC
INFLUENZA
A(H1N1)09 74
REFERENCES 77
PUBLICATIONS 83
ACKNOWLEDGEMENTS 84
|
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| id | DE-604.BV040976545 |
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| indexdate | 2024-07-10T00:36:37Z |
| institution | BVB |
| isbn | 9783863872991 |
| language | English |
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| physical | VIII, 84 S. Ill., graph. Darst. |
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| spelling | Said, Abdelrahman Fekry Abdelrahman Verfasser (DE-588)1034110039 aut Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) vorgelegt von Abdelrahman Fekry Abdelrahman Said Entwicklung eines Impstoffes gegen das pandemische Influenzavirus A (09-H1N1) basierend auf einem equinen Herpesvirus Typ 1 (EHV-1)-Vektor Berlin Mensch und Buch Verl. 2013 VIII, 84 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Entwicklung eines Impstoffes gegen das pandemische Influenzavirus A (09-H1N1) basierend auf einem equinen Herpesvirus Typ 1 (EHV-1)-Vektor Berlin, Freie Univ., Diss., 2013 Equid herpesvirus 1 cabt vectors cabt vaccines cabt Influenza A virus cabt recombination cabt western blotting cabt immunofluorescence cabt animal experiments cabt Impfstoff (DE-588)4026655-2 gnd rswk-swf Equines Herpesvirus 1 (DE-588)4320759-5 gnd rswk-swf Schweineinfluenza (DE-588)7679861-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Schweineinfluenza (DE-588)7679861-6 s Impfstoff (DE-588)4026655-2 s Equines Herpesvirus 1 (DE-588)4320759-5 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000094101-7 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000094101 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025954635&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Said, Abdelrahman Fekry Abdelrahman Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) Equid herpesvirus 1 cabt vectors cabt vaccines cabt Influenza A virus cabt recombination cabt western blotting cabt immunofluorescence cabt animal experiments cabt Impfstoff (DE-588)4026655-2 gnd Equines Herpesvirus 1 (DE-588)4320759-5 gnd Schweineinfluenza (DE-588)7679861-6 gnd |
| subject_GND | (DE-588)4026655-2 (DE-588)4320759-5 (DE-588)7679861-6 (DE-588)4113937-9 |
| title | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) |
| title_alt | Entwicklung eines Impstoffes gegen das pandemische Influenzavirus A (09-H1N1) basierend auf einem equinen Herpesvirus Typ 1 (EHV-1)-Vektor |
| title_auth | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) |
| title_exact_search | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) |
| title_full | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) vorgelegt von Abdelrahman Fekry Abdelrahman Said |
| title_fullStr | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) vorgelegt von Abdelrahman Fekry Abdelrahman Said |
| title_full_unstemmed | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) vorgelegt von Abdelrahman Fekry Abdelrahman Said |
| title_short | Development of a vectored equine herpesvirus type 1 (EHV-1) vaccine against pandemic influenza A virus (09-H1N1) |
| title_sort | development of a vectored equine herpesvirus type 1 ehv 1 vaccine against pandemic influenza a virus 09 h1n1 |
| topic | Equid herpesvirus 1 cabt vectors cabt vaccines cabt Influenza A virus cabt recombination cabt western blotting cabt immunofluorescence cabt animal experiments cabt Impfstoff (DE-588)4026655-2 gnd Equines Herpesvirus 1 (DE-588)4320759-5 gnd Schweineinfluenza (DE-588)7679861-6 gnd |
| topic_facet | Equid herpesvirus 1 vectors vaccines Influenza A virus recombination western blotting immunofluorescence animal experiments Impfstoff Equines Herpesvirus 1 Schweineinfluenza Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000094101 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025954635&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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