Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 131 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Kohler, Julia |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging |c von Julia Kohler |
| 264 | 1 | |c 2012 | |
| 300 | |a 131 S. |b Ill., graph. Darst. | ||
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| 502 | |a München, Univ., Diss., 2012 | ||
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENT TABLE 3^
C O N T E N T T A B L E
1. INTRODUCTION ......... 6
1.1. GENERAL BACKGROUND ON TENDONS 6
1.1.1. TENDON ANATOMY 6
1.1.2. TENDON ARCHITECTURE AND MOLECULAR COMPOSITION 7
1.1.3. TENDON CELLULAR CONTENT 10
1.1.3.1. HUMAN MESENCHYMAL STEM CELLS 10
1.1.3.2. STEM CELL NICHES AND DISCOVERY O F TENDON STEM/PROGENITOR CELLS
13
1.1.4. TENDON BLOOD SUPPLY AND INNERVATION 16
1.1.5. TENDON BIOMECHANICS 17
1.1.6. TENDON DEVELOPMENT 18
1.2. TENDON CLINICAL BACKGROUND 2 0
1.2.1. TENDON DISORDERS 2 0
1.2.2. TENDON PATHOPHYSIOLOGY 22
1.2.3. THE PROCESS O F AGING 2 4
1.3. THERAPY O F TENDON INJURIES 2 7
1.3.1. TENDON HEALING 2 7
1.3.2. CURRENT MANAGEMENT O F TENDON INJURIES 3 0
2. AIM.AND GOALS OF THE THESIS 33
3. MATERIALS AND METHODS 34
3.1. ISOLATION O F TSPC FROM ACHILLES TENDONS 3 4
3.2. CULTURE CONDITIONS 3 4
3.2.1. CELL CULTURE CONDITIONS 3 5
3.2.2. PASSAGING AND COUNTING 3 5
3.2.3. CRYOPRESERVATION 3 5
3.3. RNA AND COPY DNA PREPARATION 3 6
3.3.1. TOTAL RNA ISOLATION 3 6
3.3.2. CDNA SYNTHESIS 3 6
3.4. SEMI-QUANTITATIVE RT-PCR 3 6
HTTP://D-NB.INFO/1034234935
IMAGE 2
CONTENT TABLE
3.5. REAL TIME QUANTITATIVE PCR 3 7
3.6. GENOME-WIDE GENE EXPRESSION PROFILING 3 7
3.7. FLOW CYTOMETRY (FACS) 3 8
3.8. PROLIFERATION ASSAYS 3 9
3.8.1. POPULATION DOUBLINGS 3 9
3.8.2. WST-I ASSAY 3 9
3.8.3. COLONY-FORMING-UNIT ASSAY 4 0
3.9. SENESCENCE-ASSOCIATED P-GALACTOSIDASE ACTIVITY ASSAY 4 0
3.10. TIME-LAPSE EXPERIMENTS 41
3.11. DIFFERENTIATION 4 2
3.11.1. ADIPOGENIC DIFFERENTIATION 4 2
3.11.2. OSTEOGENIC DIFFERENTIATION 4 3
3.11.3. CHONDROGENIC DIFFERENTIATION 4 3
3.12. IMMUNOCYTOCHEMISTRY 4 5
3.12.1. PROTEIN COATING 4 5
3.12.2. PERHYDROL TREATMENT 4 5
3.12.3. LATRUNCULIN A TREATMENT 4 5
3.12.4. IMMUNOFLUORESCENT ACTIN CYTOSKELETAL STAINING 4 6
3.12.5. IMMUNOFLUORESCENT P I E 0 1 4 STAINING 4 6
3.13. WESTERN BLOTTING 4 7
3.13.1. PROTEIN EXTRACTION 4 7
3.13.2. SDS-PAGE 4 7
3.13.3. COOMASIE STAINING 4 8
3.13.4. PROTEIN TRANSFER 4 8
3.13.5. PROTEIN IMMUNODETECTION 4 8
3.14. FIBRONECTIN AND ROCK ELISA ASSAYS 4 9
3.14.1. FIBRONECTIN ELISA 4 9
3.14.2. ROCK ACTIVITY ELISA 4 9
3.15. MICROSCOPY 5 0
IMAGE 3
CONTENT TABLE 5
3.16. STATISTICS 51
3.17. COMPUTER PROGRAMS AND WEB LINKS 51
4. RESULTS 52
4.1. ISOLATION O F T S P C FROM ACHILLES TENDON BIOPSIES 52
4.2. CHARACTERIZATION O F T S P C 5 3
4.2.1. EXPRESSION O F MSC-RELATED SURFACE MARKERS 5 3
4.2.2. EXPRESSION O F TENDON-RELATED GENE MARKERS 55
4.2.3. LONG-TERM PROLIFERATION ANALYSIS AND CLONOGENICITY 5 7
4.2.4. DIFFERENTIATION TOWARDS ADIPOGENIC, OSTEOGENIC AND CHONDROGENIC
LINEAGES 5 9
4.3. AGE-RELATED ABERRATIONS O F T S P C 6 3
4.3.1. CELL PROLIFERATION UPON STIMULATION WITH TGF-PI 6 4
4.3.2. INVESTIGATION O F SENESCENCE-ASSOCIATED P-GALACTOSIDASE POSITIVE
CELLS 6 5
4.3.3. EXPRESSION O F CELL CYCLE REGULATORS 6 8
4.3.4. INVESTIGATION O F THE MIGRATORY CAPACITY 6 9
4.3.5. COMPARISON O F CELL AREA AND ACTIN CYTOSKELETON ORGANIZATION 72
4.3.6. INVESTIGATION O F ACTIN CYTOSKELETON DYNAMICS 7 5
4.3.7. TRANSCRIPTOME PROFILING O F YOUNG AND AGED TSPC 7 9
4.3.8. INVESTIGATION O F GENES INVOLVED IN CELL ADHESION AND ACTIN
STRESS FIBRE FORMATION 8 0
5. DISCUSSION 83
6. CONCLUSIONS 103
7. SUMMARY 105
8.
ZUSAMMENFASSUNG.........................................................................................................................
107
9. LITERATURE .... .... 109
10. APPENDIX 127
11. LIST OF ABBREVIATIONS 129
12. ACKNOWLEDGEMENTS ... 132
|
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| genre_facet | Hochschulschrift |
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| illustrated | Illustrated |
| indexdate | 2024-07-10T00:36:36Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025953519 |
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| physical | 131 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
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| spelling | Kohler, Julia Verfasser aut Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging von Julia Kohler 2012 131 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2012 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025953519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kohler, Julia Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging |
| subject_GND | (DE-588)4113937-9 |
| title | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging |
| title_auth | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging |
| title_exact_search | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging |
| title_full | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging von Julia Kohler |
| title_fullStr | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging von Julia Kohler |
| title_full_unstemmed | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging von Julia Kohler |
| title_short | Phenotypic and transcriptomal alterations in tendon stem/progenitor cells during the process of aging |
| title_sort | phenotypic and transcriptomal alterations in tendon stem progenitor cells during the process of aging |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025953519&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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