Role of caspase-8 for apoptosis induction in tumor cells:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IV, 106 Bl. graph. Darst. |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
1. INTRODUCTION 1.1. HISTORY OF CANCER THERAPY
1.2. CYTOSTATIC GROUPS AND THEIR MECHANISMS
1.3. APOPTOSIS SIGNALING PATHWAYS
1.3.1. EXTRINSIC APOPTOSIS PATHWAY
1.3.2. INTRINSIC APOPTOSIS PATHWAY
1.3.3. CROSS TALK BETWEEN THE TWO PATHWAYS
1.4. REGULATION OF THE APOPTOSIS SIGNALING PATHWAY
1.5. PRELIMINARY WORK OF THE LABORATORY AND BASIS FOR THE PRESENTED M D
THESIS...
1.6. RESEARCH OBJECTIVE
2. MATERIALS AND METHODS
2.1. MATERIALS
2.1.1. CELL CULTURE
2.1.2. CYTOTOXIC DRUGS AND INHIBITORS
2.1.2.1. CYTOTOXIC DRUGS
2.1.2.2. INHIBITORS AND REAGENTS
2.1.3. BLOCKADE OF TRAIL- AND OF FAS- SIGNALING
2.1.4. PROTEASE INHIBITORS
2.1.5. WESTERN BLOT
2.1.6. ANTIBODIES
2.1.6.1. PRIMARY ANTIBODY
2.1.6.2. SECONDARY ANTIBODY
2.1.7. BUFFER
2.1.8. OTHER MATERIALS
2.1.9. EQUIPMENT
2.2. METHODS
2.2.1. CELL CULTURE
2.2.1.1. LEUCEMIA CELL LINES AND ADHERENT CELL LINES
2.2.1.2. LONG-TERM CELL CULTURE
2.2.1.3. STIMULATION EXPERIMENTS
2.2.1.3.1. SEEDING
2.2.1.3.2. STIMULATION
2.2.1.3.3. CYTOTOXIC DRUGS
2.2.1.3.4. MEASUREMENT OF APOPTOSIS
..1
..1
..2
..3
..4
..6
. . 9
11
.15
.17
,18
. 1 8
.18
.18
18
18
.19
.19
.19
.20
20
20
.20
.22
.22
.23
.23
23
24
25
25
25
25
26
II
HTTP://D-NB.INFO/1035783002
IMAGE 2
2.2.1.4. INHIBITION EXPERIMENTS 26
2.2.1.5. PLASMIDS AND TRANSFECTION EXPERIMENTS 26
2.2.2. MEASUREMENT OF APOPTOSIS 28
2.2.2.1. FLUORESCENCE ACTIVATED CELL SORTING (FACS) 28
2.2.2.2. ANALYSIS OF FACSCAN MEASUREMENT 30
2.2.3. STATISTICAL ANALYSIS 30
2.2.4. CELL LYSIS 31
2.2.5. DETERMINATION OF PROTEIN CONCENTRATION IN CELLULAR LYSATES 31
2.2.6. SEPARATION OF PROTEINS BY DENATURING DISCONTINUOUS POLYACRYLAMIDE
GEL
ELECTROPHORESIS (PAGE) 32
2.2.7. ANALYSIS OF PROTEINS ON NITROCELLULOSE MEMBRANE BY IMMUNOBLOTTING
32
3. RESULTS 34
3.1. ROLE OF CASPASE-8 IN MTX-MEDIATED SENSITIZATION FOR T R A I L -
INDUCED
APOPTOSIS 3 4
3.1.1. REGULATION OF CASPASE-8 BY MTX IN LEUKAEMIA CELLS 34
3.1.2. MTX-INDUCED UPREGULATION OF CASPASE-8 AND SENSITIZATION FOR
TRAIL-INDUCED
APOPTOSIS 38
3.1.3. MOLECULAR INHIBITION OF CASPASE-8 PROTEIN UPREGULATION 42
3.2. ROLE OF P53 IN UPREGULATION OF CASPASE-8 AND RESTORATION OF T R A I
L
SENSITIVITY 4 5
3.2.1. EFFECT OF MTX ON ACTIVATION OF TRANSCRIPTION FACTORS 46
3.2.2. INHIBITION OF MTX-INDUCED SENSITIZATION TOWARDS TRAIL-LNDUCED
APOPTOSIS BY
INHIBITION OF P53 48
3.2.2.1. BIOCHEMICAL INHIBITION OF P53 48
3.2.2.2. MOLECULAR INHIBITION OF P53 50
3.2.3. INVESTIGATION OF FURTHER P53 ACTIVATORS AS POTENTIAL REGULATORS
OF CASPASE-8 53
3.2.4. P53-MEDIATED UPREGULATION OF CASPASE-8 BY 5-FLUOROURACIL 55
3.3. ROLE OF CASPASE-8 IN CYTOTOXIC DRUG INDUCED APOPTOSIS 5 9
3.3.1. DEATH RECEPTOR-INDEPENDENT APOPTOSIS INDUCTION BY CYTOTOXIC DRUGS
60
3.3.2. BIOCHEMICAL INHIBITION OF CASPASE-8 63
3.3.3. EFFECT OF DOWNREGULATION OF CASPASE-8 BY SHRNA INTERFERENCE ON
DRUG
SENSITIVITY 66
3.3.4. SENSITIZATION FOR DRUG-INDUCED APOPTOSIS BY MTX VIA CASPASE-8
UPREGULATION 70
3.3.5. MTX-INDUCED SENSITIZATION FOR DRUG-INDUCED APOPTOSIS DEPENDS ON
CASPASE-8 AND
P53 : 75
III
IMAGE 3
4. DISCUSSION 79
5. ABSTRACT 89
6. ZUSAMMENFASSUNG 90
6. ABBRIVIATIONS 91
7. REFERENCE 92
8. DANKSAGUNG 106
9. CV 107
IV
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| indexdate | 2024-07-10T00:36:35Z |
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| language | English |
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| spelling | Maurer, Martina Anna Catharina Verfasser (DE-588)1034090607 aut Role of caspase-8 for apoptosis induction in tumor cells von Martina Anna Catharina Maurer 2013 IV, 106 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025953362&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Maurer, Martina Anna Catharina Role of caspase-8 for apoptosis induction in tumor cells |
| subject_GND | (DE-588)4113937-9 |
| title | Role of caspase-8 for apoptosis induction in tumor cells |
| title_auth | Role of caspase-8 for apoptosis induction in tumor cells |
| title_exact_search | Role of caspase-8 for apoptosis induction in tumor cells |
| title_full | Role of caspase-8 for apoptosis induction in tumor cells von Martina Anna Catharina Maurer |
| title_fullStr | Role of caspase-8 for apoptosis induction in tumor cells von Martina Anna Catharina Maurer |
| title_full_unstemmed | Role of caspase-8 for apoptosis induction in tumor cells von Martina Anna Catharina Maurer |
| title_short | Role of caspase-8 for apoptosis induction in tumor cells |
| title_sort | role of caspase 8 for apoptosis induction in tumor cells |
| topic_facet | Hochschulschrift |
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