Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 185 S. Ill., graph. Darst. 21 cm |
Internformat
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| 100 | 1 | |a Sehlbach, Maria |d 1983- |e Verfasser |0 (DE-588)1030605610 |4 aut | |
| 245 | 1 | 0 | |a Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds |c vorgelegt von Maria Sehlbach |
| 264 | 1 | |c 2012 | |
| 300 | |a 185 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Münster (Westfalen), Univ., Diss., 2013 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025930245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
I N D E X
I N D E X
LIST O F A B B R E V I A T I O N S 6
S U M M A R Y 9
I-INTRODUCTION 13
1.1 - - J A T R O P H A C U R C A S L. - 13
1.2 - - A R A B I N O G A L A C T A N P R O T E I N S (AGPS) 15
1.2.1 - P R O T E I N C O R E O F AGP - 1 5
1 . 2 . 2 - C A R B O H Y D R A T E M O I E T Y O F AGP 16
1.2.3-FUNCTION O F AGPS IN P L A N T S 18
1 . 2 . 4 - U S A G E O F AGPS A N D P O S S I B L E A P P L I C A T I O
N S - 19
1.3 - - R A W P O L Y S A C C H A R I D E S F R O M J. C U R C A S S E E
D S I N D U C E W O U N D H E A L I N G E F F E C T S 19
1 . 4 - A I M O F W O R K 2 0
2 RESULTS 21
2.1 --ISOLATION O F R A W P O L Y S A C C H A R I D E S F R O M J A T R
O P H A C U R C A S L. S E E D S 21
2 . 2 - T E S T O N S T A R C H - 21
2 . 3 - F R A C T I O N A T I O N O F T H E R A W P O L Y S A C C H A R
I D E S (JCI) O F J. C U R C A S B Y U S E O F I O N - E X C H A N G E
C H R O M A T O G R A P H Y (IEC) 21
2 . 4 -TESTS T O I N C R E A S E S O L U B I L I T Y 23
2.5-ANALYTICAL I N V E S T I G A T I O N S O N T H E IEC F R A C T I O N
S2 - 2 4
2 . 5 . 1 - C A R B O H Y D R A T E C O N T E N T O F S2 - 2 4
2.5.2-PROTEIN D E T E R M I N A T I O N IN S2 - - 2 4
2 . 5 . 3 - A R A B I N O G A L A C T A N P R O T E I N D E T E R M I N
A T I O N IN S2 B Y G E L D I F F U S I O N T E S T W I T H - D
GLUCOSYL YARIV R E A G E N T - 25
2 . 5 . 4 - M O L E C U L A R W E I G H T D E T E R M I N A T I O N B Y
G E L P E R M E A T I O N C H R O M A T O G R A P H Y (GPC) - 2 6
2.5.5-ANALYSIS O N S2 B Y G E L E L E C T R O P H O R E S I S (SDS-PAGE)
O F S2 - - 2 7
2.6 - D E N A T U R I N G O F C O - E X T R A C T E D P R O T E I N IN T
H E R A W P O L Y S A C C H A R I D E S 2 8
2.6.1-ANALYTICAL I N V E S T I G A T I O N S O N H E A T - D E P L E T E
D R A W P O L Y S A C C H A R I D E JC2 2 9
2.6.2 - Q U A N T I T A T I V E D E T E R M I N A T I O N O F U R O N I
C A C I D S IN JC2 2 9
2 . 7 - F R A C T I O N A T I O N O F JC2 B Y I O N - E X C H A N G E C
H R O M A T O G R A P H Y - - 2 9
2.8 -ANALYTICAL I N V E S T I G A T I O N S O N R2 - 3 0
2.8.1-SDS-PAGE O F R2 - - 31
2.8.2 - M O L E C U L A R W E I G H T D E T E R M I N A T I O N O F R2 B
Y H I G H - P E R F O R M A N C E SIZE E X C L U S I O N
C H R O M A T O G R A P H Y - - 3 2
1
HTTP://D-NB.INFO/1031134816
IMAGE 2
I N D E X
2.8.3 - Q U A L I T A T I V E A N D Q U A N T I T A T I V E M O N O S A
C C H A R I D E D E T E R M I N A T I O N O F R2 A F T E R
HYDROLYSIS BY HPAEC-PAD 35
2.8.3.1 - A C I D I C HYDROLYSIS - - - 35
2.8.3.2-HPAEC-PA D 3 6
2 . 8 . 3 . 3 - M O N O S A C C H A R I D E S O F IEC F R A C T I O N R2
- 37
2.9 -P-D-GLUCOSYL YARIV R E A G E N T 41
2.9.1-SYNTHESIS O F |5-D-GLUCOSYL YARIV R E A G E N T 4 3
2.9.2 - A N A L Y T I C A L C H A R A C T E R I S A T I O N O F
|3-D-GLUCOSYL YARIV R E A G E N T - 4 4
2.10 I S O L A T I O N O F A R A B I N O G A L A C T A N P R O T E I N S
F R O M R2 B Y P R E C I P I T A T I O N W I T H P-GLCY 52
2.11 -ANALYTICAL I N V E S T I G A T I O N S O N T H E AGP F R A C T I O
N Y2 - 52
2.11.1 - E L E M E N T A L A N A L Y S I S B Y T O T A L R E F L E C T I
O N X - R A Y F L U O R E S C E N C E (TXRF) 52
2.11.2-SDS-PAGE O F Y2 - 53
2 . 1 1 . 3 - M O L E C U L A R SIZE D E T E R M I N A T I O N B Y
HP-SEC A N D GPC 5 4
2 . 1 1 . 4 - M O N O S A C C H A R I D E D E T E R M I N A T I O N O F
Y2 B Y HPAEC-PAD A N D GC-FID 5 6
2.11.4.1 - Q U A L I T A T I V E A N D Q U A N T I T A T I V E A N A L Y
S I S O N M O N O S A C C H A R I D E S B Y HPAEC-PAD 5 6
2.11.4.2-OUALITATIVE A N D Q U A N T I T A T I V E A N A L Y S I S O N M
O N O S A C C H A R I D E S B Y GC-FID 5 8
2.11.5-CAPILLARY Z O N E E L E C T R O P H O R E S I S F O R D E T E R M
I N A T I O N O F D- A N D L - C O N F I G U R A T I O N O F
M O N O S A C C H A R I D E S IN Y2 6 0
2 . 1 1 . 6 - M E T H Y L A T I O N A N A L Y S I S F O R D E T E R M I
N A T I O N O F C A R B O H Y D R A T E L I N K A G E 61
2 . N . 7 ~ A - / P - A N O M E R I C L I N K A G E I N V E S T I G A T
I O N S O N Y2 B Y NMR 6 7
2.12-STRUCTURAL I N V E S T I G A T I O N S O N Y2 B Y C A R B O H Y D R
A T E E X O / E N D O - E N Z Y M A T I C C L E A V A G E 73
2.13-LSOLATION O F A P - ( I - 3 ) - G A L A C T A N A S E F R O M
DRISELASE - 75
2.14 E N Z Y M A T I C D E G R A D A T I O N O F Y2 BY DRISELASE F R A
C T I O N D2 T O SPECIFIC O L I G O S A C C H A R I D E S
A N D T H E I R I D E N T I F I C A T I O N BY M S - 7 7
2.15-PROPOSED S T R U C T U R E O F T H E C A R B O H Y D R A T E M O I
E T Y IN Y2 9 0
2.16 A M I N O A C I D D E T E R M I N A T I O N B Y HPAEC-PAD - 9 2
2.17-DEGLYCOSYLATION O F T H E P R O T E I N P A R T O F Y 2 9 3
2.17.1 - A L K A L I N E ^ - E L I M I N A T I O N 9 3
2.17.2-DEGLYCOSYLATION B Y T R I F L U O R O M E T H A N E S U L P H O N
I C A C I D 9 5
2.17.3-DEGLYCOSYLATION B Y E N D O G L Y C O S I D A S E S P N G A S E F
A N D N - G L Y C O S I D A S E A 9 6
2.18 PARTIAL S E Q U E N C I N G O F P E P T I D E S A F T E R T R Y P T
I C D I G E S T O F Y2 1 0 0
2.18.1-FASCIDIN-LIKE A R A B I N O G A L A C T A N P R O T E I N S 101
2.18.2 -XYLOGEN-LIKE P R O T E I N 107
2.18.3 -LYSM D O M A I N - C O N T A I N I N G P R O T E I N - 111
2
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2.19 TEST F O R GPI A N C H O R S IN Y2 115
2 . 2 O S E E D S E C T I O N I N G 115
3 DISCUSSION 119
3.1 - S T R U C T U R E A N D F U N C T I O N O F AGPS IN Y2 119
3.1.1 -XYLOGEN-LIKE P R O T E I N - 120
3.1.2-FASCIDIN-LIKE AGP 121
3.1.3- LYSM D O M A I N - C O N T A I N I N G P R O T E I N 122
3 . 2 - G E N E R A L P H Y T O C H E M I C A L P R O C E D U R E O F S
T R U C T U R A L I N V E S T I G A T I O N S O F AGPS 124
4 MATERIAL A N D M E T H O D S 129
4 . 1 - C O M M O N L A B O R A T O R Y E Q U I P M E N T A N D I N S T
R U M E N T S 129
4.2 - C O N S U M A B L E G O O D S 130
4.3 - C H E M I C A L S 131
4 . 4 - D R U G M A T E R I A L 133
4.5 - G E N E R A L M E T H O D S 133
4 . 6 ISOLATION A N D P U R I F I C A T I O N - 134
4.6.1 - E X T R A C T I O N O F J A T R O P H A C U R C A S L. S E E D S
134
4 . 6 . 2 - H E A T - D E P L E T I O N O F C O - E X T R A C T E D P R
O T E I N - 134
4 . 6 . 3 - I S O L A T I O N O F A R A B I N O G A L A C T A N P R O T
E I N S W I T H (5-D-GLUCOSYL YARIV R E A G E N T ( K R E U G E R
A N D VAN HOIST, 1993; 1995) 134
4 . 6 . 4 - L S O L A T I O N O F A S O L U B L E AGP C O N T A I N I N
G G U M A R A B I C F R A C T I O N ( D E F A Y E A N D W O N G ,
1 9 8 6 ) - 135
4.7 - C O L O R I M E T R I C M E T H O D S 135
4 . 7 . 1 - D E T E R M I N A T I O N O F C A R B O H Y D R A T E S BY R
E S O R C I N O L S U L P H U R I C A C I D M I C R O M E T H O D
( M O N S I G N Y E T AL., 1988) 135
4 . 7 . 2 - Q U A N T I T A T I V E D E T E R M I N A T I O N O F U R O
N I C A C I D S ( B L U M E N K R A N T Z A N D A S B O E - H A N S E N
,
1973 M O D I F I E D B Y K R A M , I G 8 4 ) 136
4 . 7 . 3 - S E M I Q U A N T I T A T I V E D E T E R M I N A T I O N O
F A R A B I N O G A L A C T A N - P R O T E I N S (AGPS) (YARIV E T
AL., 1962, M O D I F I E D B Y VAN H O I S T A N D CLARKE, 1985) 136
4.7.4-PROTEIN D E T E R M I N A T I O N (BRADFORD, 1976) - 137
4 . 7 . 5 - C O O M A S S I E BRILLIANT BLUE R-250 S T A I N I N G A F T
E R SDS-PAGE ( S C H A G G E R , 2 0 0 6 ) 137
4.7.6-COLLOIDAL C O O M A S S I E S T A I N I N G A F T E R SDS-PAGE -
137
4.7.7 - Y A R I V S T A I N I N G A F T E R SDS-PAGE ( H E R R M A N N ,
2 0 0 9 ) 138
4.7.8 - S I L V E R S T A I N I N G A F T E R SDS-PAGE ( H E U K E S H O
V E N A N D D E R N I C K , I G 8 8 ) 138
4.7.9 - H I S T O L O G I C A L I N V E S T I G A T I O N S : AGP S T A
I N I N G IN J . C U R C A S S E E D S 139
4.7.10-TEST O N S T A R C H - 139
3
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4 . 7 . 1 1 - D E T E C T I O N O F C A R B O H Y D R A T E S A F T E R
T L C S E P A R A T I O N W I T H T H Y M O L - S U L P H U R I C A C I
D
R E A G E N T - - 139
4 . 8 -HYDROLYSIS M E T H O D S 1 4 0
4.8.1 - H Y D R O L Y S I S O F P O L Y S A C C H A R I D E S W I T H T
R I F L U O R O A C E T I C A C I D 1 4 0
4 . 8 . 2 - H Y D R O L Y S I S O F P O L Y S A C C H A R I D E S T O O
L I G O S A C C H A R I D E S 1 4 0
4 . 8 . 3 - H Y D R O L Y S I S O F P E P T I D E S W I T H H Y D R O C
H L O R I C A C I D F O R HPAEC-PAD 1 4 0
4 . 8 . 4 - H Y D R O L Y S I S O F P E P T I D E S F O R D E T E R M I
N A T I O N O F T R Y P T O P H A N B Y HPAEC-PAD 1 4 0
4 . 8 . 5 - H Y D R O L Y S I S O F D E X T R A N T O O L I G O S A C C
H A R I D E S 1 4 0
4 . 8 . 6 - A C E T O L Y S I S O F P H O S P H O L I P I D S ( R O B E
R T S E T AL., 1 § 8 8 ) 141
4 . 9 - D E R I V A T I S A T I O N M E T H O D S A N D R E D U C T I O
N P R O C E D U R E S 141
4.9.1 - A C E T Y L A T I O N O F M O N O S A C C H A R I D E S ( B L A
K E N E Y E T AL., 1983) 141
4 . 9 . 2 - R E D U C T I O N O F ACIDIC P O L Y S A C C H A R I D E S
(TAYLOR A N D C O N R A D , 1972) - 142
4 . 9 . 3 - M E T H Y L A T I O N A N A L Y S I S O F P O L Y S A C C H
A R I D E S ( H A K O M O R I , 1 9 6 4 , M O D I F I E D B Y H A R R I
S E T
A L . , 1 9 8 4 ) 142
4 . 9 . 4 - D E R I V A T I S A T I O N W I T H S - ( - ) - I - P H E N
Y L E T H Y L A M I N E ( N O E A N D F R E I S S M U T H , I 9 9 5 )
144
4.10 M E T H O D S F O R C H R O M A T O G R A P H Y 144
4.10.1 -THIN LAYER C H R O M A T O G R A P H Y - - 144
4 . 1 0 . 2 - L O N - E X C H A N G E C H R O M A T O G R A P H Y 144
4.10.3-GEL P E R M E A T I O N C H R O M A T O G R A P H Y 1 4 6
4 . 1 0 . 4 - H I G H - P E R F O R M A N C E A N I O N - E X C H A N G
E C H R O M A T O G R A P H Y W I T H P U L S E D A M P E R O M E T R I
C
D E T E C T I O N (HPAEC-PAD) 146
4.10.4.1 C A R B O H Y D R A T E D E T E R M I N A T I O N - 147
4 . 1 0 . 4 . 2 A M I N O A C I D D E T E R M I N A T I O N 1 4 9
4.10.5-GAS C H R O M A T O G R A P H Y - - 151
4.10.5.1 GC-FID 151
4.10.5.2 GC-MS 152
4.10.6-HIGH P E R F O R M A N C E SIZE E X C L U S I O N C H R O M A T O
G R A P H Y (HP-SEC) 154
4.10.7-SOLID P H A S E E X T R A C T I O N O F O L I G O S A C C H A R I
D E S O N G R A P H I T I S E D C A R B O N 155
4.10.8-LIQUID C H R O M A T O G R A P H Y C O U P L E D W I T H M A S S
S P E C T R O M E T R Y 155
4.10.8.1 UHPLC-ESI-O-TOF-MS 155
4.10.8.2 O-TOF P R E M I E R N A N O A C Q U I T Y LC 158
4.11-DEGLYCOSYLATION M E T H O D S - 159
4.11.1 - D E G L Y C O S Y L A T I O N B Y T R I F L U O R O M E T H A N
E S U L P H O N I C A C I D (EDGE E T AL., 1981; LIND E T AL.,
1994; B O S C H E T AL., 2001) - 159
4 . 1 1 . 2 - P N G A S E F N - D E G L Y C O S Y L A T I O N - 159
4
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4 . 1 1 . 3 - N - G L Y C O S I D A S E A N - D E G L Y C O S Y L A T I
O N --159
4 . 1 1 . 4 - ^ - E L I M I N A T I O N O F O-GLYCOSIDIC LINKED P O L Y
S A C C H A R I D E S 1 6 0
4.12 E L E C T R O P H O R E T I C M E T H O D S 161
4.12.1 -TRIS-SDS-PAGE ( L A E M M L I , 1970) 161
4.12.2-TRICINE-SDS-PAGE ( S C H A G G E R , 2 0 0 6 ) 162
4.12.3 -CAPILLARY Z O N E E L E C T R O P H O R E S I S - - 1 6 4
4.13 E N Z Y M A T I C M E T H O D S 165
4_I3.I--DIGEST O F P O L Y S A C C H A R I D E S W I T H E X O - A N D E
N D O - E N Z Y M E S 165
4 . 1 3 . 2 - D I G E S T O F G U M A R A B I C W I T H E X O - A R A B
I N O F U R A N O S I D A S E 1 6 6
4.13.3-ISOLATION O F A P - ( I - 3 ) - G A L A C T A N A S E F R O M
DRISELASE ( M O D I F I E D P R O C E D U R E T O
T S U M U R A Y A E T A L . , 1 9 9 0 ) 1 6 6
4 . 1 3 . 4 - F I - ( I - 3 ) - G A L A C T A N A S E D I G E S T O F
AGP T O O L I G O S A C C H A R I D E S 167
4.13.5-TRYPTIC IN-GEL D I G E S T I O N O F P R O T E I N 167
4 / 1 3 . 6 - P N G A S E F N - D E G L Y C O S Y L A T I O N 1 6 8
4.13.7 - N - G L Y C O S I D A S E A N - D E G L Y C O S Y L A T I O N 1
6 8
4.14 S P E C T R O M E T R I C A N D S P E C T R O S C O P I C M E T H O
D S 1 6 8
4.14.1 -NANOESI-O-TOF M S / M S 1 6 8
4 . 1 4 . 2 - N U D E A R M A G N E T I C R E S O N A N C E S P E C T R
O S C O P Y (NMR) 1 6 8
4.14.3-TOTAL R E F L E C T I O N X-RAY F L U O R E S C E N C E (TXRF) 1
6 9
4.15 S Y N T H E S I S O F P-D-GLUCOSYL YARIV R E A G E N T ( A C C O R
D I N G T O YARIV E T AL., 1962) 1 6 9
4.16 S E E D S E C T I O N I N G 170
BIBLIOGRAPHY 172
PUBLICATIONS 181
A C K N O W L E D G E M E N T S 182
CURRICULUM V I T A E 185
5
|
| any_adam_object | 1 |
| author | Sehlbach, Maria 1983- |
| author_GND | (DE-588)1030605610 |
| author_facet | Sehlbach, Maria 1983- |
| author_role | aut |
| author_sort | Sehlbach, Maria 1983- |
| author_variant | m s ms |
| building | Verbundindex |
| bvnumber | BV040951709 |
| ctrlnum | (OCoLC)830890043 (DE-599)DNB1031134816 |
| dewey-full | 572.566332369 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.566332369 |
| dewey-search | 572.566332369 |
| dewey-sort | 3572.566332369 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV040951709 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:36:04Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-025930245 |
| oclc_num | 830890043 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29T |
| owner_facet | DE-355 DE-BY-UBR DE-29T |
| physical | 185 S. Ill., graph. Darst. 21 cm |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spelling | Sehlbach, Maria 1983- Verfasser (DE-588)1030605610 aut Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds vorgelegt von Maria Sehlbach 2012 185 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Münster (Westfalen), Univ., Diss., 2013 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025930245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sehlbach, Maria 1983- Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds |
| subject_GND | (DE-588)4113937-9 |
| title | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds |
| title_auth | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds |
| title_exact_search | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds |
| title_full | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds vorgelegt von Maria Sehlbach |
| title_fullStr | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds vorgelegt von Maria Sehlbach |
| title_full_unstemmed | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds vorgelegt von Maria Sehlbach |
| title_short | Structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from Jatropha curcas L. seeds |
| title_sort | structural characterisation of the carbohydrate and protein part of arabinogalactan proteins from jatropha curcas l seeds |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025930245&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sehlbachmaria structuralcharacterisationofthecarbohydrateandproteinpartofarabinogalactanproteinsfromjatrophacurcaslseeds |