Are CLCA proteins metalloproteases?: Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch & Buch
2012
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Sind CLCA-Proteine Metalloproteasen? |
| Beschreibung: | 118 S. Ill., graph. Darst. |
| ISBN: | 9783863872526 |
Internformat
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| 100 | 1 | |a Bothe, Melanie Kathrin |e Verfasser |0 (DE-588)1033448710 |4 aut | |
| 245 | 1 | 0 | |a Are CLCA proteins metalloproteases? |b Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins |c vorgelegt von Melanie Kathrin Bothe |
| 246 | 1 | 3 | |a Sind CLCA-Proteine Metalloproteasen? |
| 264 | 1 | |a Berlin |b Mensch & Buch |c 2012 | |
| 300 | |a 118 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Sind CLCA-Proteine Metalloproteasen? | ||
| 502 | |a Berlin, Freie Univ., Diss., 2012 | ||
| 650 | 7 | |a cystic fibrosis |2 cabt | |
| 650 | 7 | |a asthma |2 cabt | |
| 650 | 7 | |a chronic obstructive pulmonary disease |2 cabt | |
| 650 | 7 | |a mice |2 cabt | |
| 650 | 7 | |a metalloproteinases |2 cabt | |
| 650 | 7 | |a zinc |2 cabt | |
| 650 | 7 | |a protein transport |2 cabt | |
| 650 | 0 | 7 | |a Chloridkanal |0 (DE-588)4291189-8 |2 gnd |9 rswk-swf |
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| 912 | |a ebook | ||
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Datensatz im Suchindex
| _version_ | 1804150243228385280 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
CONTENTS VII
LIST OF ABBREVIATIONS X
1 INTRODUCTION 11
2 LITERATURE 13
2.1 TISSUE EXPRESSION PATTERNS OF CLCA PROTEINS 13
2.1.1 CLCA CLUSTER 1 14
2.1.2 CLCA CLUSTER 2 15
2.1.3 CLCA CLUSTER 3 16
2.1.4 CLCACLUSTER4 18
2.2 BIOMEDICAL RELEVANCE OF CLCA PROTEINS 19
2.2.1 DISEASES WITH SECRETORY DYSFUNCTION 19
2.2.1.1 MODULATION OF CALCIUM ACTIVATED CHLORIDE CHANNELS IN CYSTIC
FIBROSIS 19
2.2.1.2 MUCUS HYPERSECRETION IN CYSTIC FIBROSIS, ASTHMA AND CHRONIC
OBSTRUCTIVE
PULMONARY DISEASE 22
2.2.2 CELL ADHESION UNDER PHYSIOLOGIC CONDITIONS AND IN METASTASIZING
TUMOR CELLS 24
2.2.3 ROLE IN APOPTOSIS AND TUMOR GROWTH 25
2.3 STRUCTURE AND BIOCHEMICAL CHARACTERISTICS OF CLCA PROTEINS 26
2.3.1 N-CLCA DOMAIN 26
2.3.2 VON WILLEBRAND FACTOR A DOMAIN 27
2.3.3 FIBRONECTIN TYPE III DOMAIN 27
2.4 CELLULAR PROCESSING OF CLCA PROTEINS 28
2.4.1 SIGNAL SEQUENCE 28
2.4.2 GLYCOSYLATION 28
2.4.3 CLEAVAGE OF CLCA PROTEINS 29
2.4.4 SECRETION OF WHOLE PROTEIN VERSUS ECTODOMAIN SHEDDING OF
TRANSMEMBRANE
CLCA PROTEINS 30
VII
HTTP://D-NB.INFO/1032579242
IMAGE 2
CONTENTS
3 AIMS OF THIS PH.D. PROJECT 32
3.1 AIM NO. 1: CHARACTERIZATION OF THE CLEAVAGE PROCESS OF THE SECRETED
MCLCA3
PROTEIN 32
3.2 AIM NO. 2: IDENTIFICATION OF A MURINE TRANSMEMBRANE CLCA PROTEIN IN
TISSUES
RELEVANT FOR CYSTIC FIBROSIS 33
3.2.1 EXPRESSION PATTERN ANALYSIS AND BIOCHEMICAL CHARACTERIZATION OF
MCLCA5 33
3.2.2 EXPRESSION PATTERN ANALYSIS AND BIOCHEMICAL CHARACTERIZATION OF
MCLCA6 34
3.3 AIM NO. 3: COMPARISON OF THE CLEAVAGE PROCESSES OF A SECRETED CLCA
PROTEIN
VERSUS A TRANSMEMBRANE CLCA PROTEIN 34
4 RESEARCH PUBLICATIONS IN PEER-REVIEWED JOURNALS 35
4.1 MURINE MCLCA6 IS AN INTEGRAL APICAL MEMBRANE PROTEIN OF NON-GOBLET
CELL
ENTEROCYTES AND CO-LOCALIZES WITH THE CYSTIC FIBROSIS TRANSMEMBRANE
CONDUCTANCE REGULATOR 35
4.2 MURINE MCLCA5 IS EXPRESSED IN GRANULAR LAYER KERATINOCYTES OF
STRATIFIED
EPITHELIA 36
4.3 THE MURINE MCLCA3 IS A ZINC-DEPENDENT METALLOPROTEASE WITH
AUTOPROTEOLYTIC
ACTIVITY 37
4.4 IMPAIRED AUTOPROTEOLYTIC CLEAVAGE OF MCLCA6, A MURINE INTEGRAL
MEMBRANE
PROTEIN EXPRESSED IN ENTEROCYTES, LEADS TO CLEAVAGE AT THE PLASMA
MEMBRANE
INSTEAD OF CLEAVAGE IN THE ENDOPLASMIC RETICULUM 38
5 CONCLUDING DISCUSSION 83
5.1 HYPOTHESIS I: CLCA PROTEINS SHOW CHARACTERISTICS OF METALLOPROTEASES
83
5.1.1 AIM NO. 1: CHARACTERIZATION OF THE CLEAVAGE PROCESS OF THE
SECRETED MCLCA3
PROTEIN 83
5.1.2 ARE CLCA PROTEINS METALLOPROTEASES OF THE ZINCIN FAMILY? 85
5.1.2.1 HEXXH MOTIF OF METALLOPROTEASES 85
5.1.2.2 ZINC-DEPENDENT CLEAVAGE MECHANISM 86
5.1.2.3 (AUTO-)PROTEOLYTIC ACTIVITY 87
IMAGE 3
CONTENTS
5.2 HYPOTHESIS II: THE CLEAVAGE PROCESSES OF TRANSMEMBRANE AND SECRETED
CLCA
PROTEINS DIFFER IN CONSEQUENCE OF THE TRANSMEMBRANE DOMAIN 87
5.2.1 AIM NO. 2: IDENTIFICATION OF A MURINE TRANSMEMBRANE CLCA PROTEIN
IN TISSUES
RELEVANT FOR CYSTIC FIBROSIS 88
5.2.1.1 EXPRESSION PATTERN ANALYSIS OF MCLCA5 AND MCLCA6 WITH A FOCUS ON
TISSUES
RELEVANT FOR CYSTIC FIBROSIS 88
5.2.1.2 TRANSMEMBRANE DOMAINS OF MCLCA5 AND MCLCA6 89
5.2.2 AIM NO. 3: COMPARISON OF THE CLEAVAGE PROCESSES OF THE SECRETED
MCLCA3
AND THE TRANSMEMBRANE MCLCA6 PROTEINS 90
5.3 POSSIBLE ROLE OF CLUSTER 1 CLCA PROTEINS AS METALLOPROTEASES IN
DISEASE 92
5.3.1 DISEASES WITH SECRETORY DYSFUNCTION (CYSTIC FIBROSIS, ASTHMA,
CHRONIC OBSTRUCTIVE
PULMONARY DISEASE) 92
5.3.1.1 CLCA PROTEASES IN MODULATION OF CHLORIDE SECRETION 92
5.3.1.2 CLCA PROTEASES IN MUCUS HYPERSECRETION 93
5.3.2 CLCA PROTEASES IN ADHESION OF METASTASIZING TUMOR CELLS AND
LYMPHOCYTES 94
5.3.3 CLCA PROTEASES IN TUMOR GROWTH / APOPTOSIS 94
5.4 CO NCLUSION AND OUTLOOK 95
6 SUMMARY 96
7 ZUSAMMENFASSUNG 99
8 REFERENCES 102
9 TALKS AND POSTER PRESENTATIONS 115
10 ACKNOWLEDGMENTS I DANKSAGUNG 117
SELBSTANDIGKEITSERKLARUNG 118
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| indexdate | 2024-07-10T00:35:49Z |
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| isbn | 9783863872526 |
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| spelling | Bothe, Melanie Kathrin Verfasser (DE-588)1033448710 aut Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins vorgelegt von Melanie Kathrin Bothe Sind CLCA-Proteine Metalloproteasen? Berlin Mensch & Buch 2012 118 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Sind CLCA-Proteine Metalloproteasen? Berlin, Freie Univ., Diss., 2012 cystic fibrosis cabt asthma cabt chronic obstructive pulmonary disease cabt mice cabt metalloproteinases cabt zinc cabt protein transport cabt Chloridkanal (DE-588)4291189-8 gnd rswk-swf Mukoviszidose (DE-588)4040669-6 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Mukoviszidose (DE-588)4040669-6 s Chloridkanal (DE-588)4291189-8 s Zellkultur (DE-588)4067547-6 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000094034-8 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000094034 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025919497&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bothe, Melanie Kathrin Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins cystic fibrosis cabt asthma cabt chronic obstructive pulmonary disease cabt mice cabt metalloproteinases cabt zinc cabt protein transport cabt Chloridkanal (DE-588)4291189-8 gnd Mukoviszidose (DE-588)4040669-6 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4291189-8 (DE-588)4040669-6 (DE-588)4067547-6 (DE-588)4113937-9 |
| title | Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins |
| title_alt | Sind CLCA-Proteine Metalloproteasen? |
| title_auth | Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins |
| title_exact_search | Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins |
| title_full | Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins vorgelegt von Melanie Kathrin Bothe |
| title_fullStr | Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins vorgelegt von Melanie Kathrin Bothe |
| title_full_unstemmed | Are CLCA proteins metalloproteases? Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins vorgelegt von Melanie Kathrin Bothe |
| title_short | Are CLCA proteins metalloproteases? |
| title_sort | are clca proteins metalloproteases comparison of the cleavage processes of the secreted mclca3 and the transmembrane mclca6 proteins |
| title_sub | Comparison of the cleavage processes of the secreted mCLCA3 and the transmembrane mCLCA6 proteins |
| topic | cystic fibrosis cabt asthma cabt chronic obstructive pulmonary disease cabt mice cabt metalloproteinases cabt zinc cabt protein transport cabt Chloridkanal (DE-588)4291189-8 gnd Mukoviszidose (DE-588)4040669-6 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | cystic fibrosis asthma chronic obstructive pulmonary disease mice metalloproteinases zinc protein transport Chloridkanal Mukoviszidose Zellkultur Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000094034 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025919497&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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