ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana:
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Format: | Abschlussarbeit Buch |
Sprache: | English |
Veröffentlicht: |
2012
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Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | 121 Bl. Ill., graph. Darst. |
Internformat
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adam_text | IMAGE 1
TABLE OF CONTENTS
LIST O F FIGURES AND TABLES 8
LIST O F ABBREVIATIONS 10
SUMMARY 11
CHAPTER 1. GENERAL INTRODUCTION 13
1.1 THE EUDICOT LEAF AND TRANSCRIPTIONAL MODULES 13
1.1.1 ESTABLISHMENT O F LEAF PRIMORDIA AND CELL PROLIFERATION 14
1.1.2 ABAXIAL VERSUS ADAXIAL IDENTITY 16
1.1.3 CELL EXPANSION 17
1.1.4 NON-SYMMETRIC LEAF GROWTH 18
1.2 LEAF MORPHOLOGY AND CELL DIFFERENTIATION 19
1.2.1 EPIDERMIS 19
1.2.2 TRICHOMES 20
1.2.3 MESOPHYLL CELLS 21
1.2.4 STOMATA 21
1.3 ENDOGENOUS SIGNALS AFFECTING LEAF GROWTH 22
1.3.1 ROLE O F AUXIN (PRIMORDIA, VASCULAR NETWORK) 22
1.3.2 ROLE O F CYTOKININ (CELL DIVISION) 22
1.3.3 REACTIVE OXYGEN SPECIES (ROS) 23
1.3.4 ETHYLENE 24
1.3.5 BRASSINOSTEROIDS AND GIBBERELLINS 24
1.3.6 GLUCOSE INTERACTS WITH PLANT HORMONES 25
1.4 CONCLUDING REMARKS 26
1.5 AIMS O F THIS THESIS 26
CHAPTER 2. MATERIAL AND METHODS 27
2.1 GENERAL 27
2.2 PLANT MATERIAL AND GROWTH CONDITIONS 27
2.3 CONSTRUCTION O F PLASMIDS FOR PLANT TRANSFORMATION 27
4
HTTP://D-NB.INFO/1036625966
IMAGE 2
2.4 PLANT TRANSFORMATION AND SELECTION O F TRANSGENIC PLANTS 28
2.5 EXPRESSION PROFILING BY QUANTITATIVE RT-PCR 29
2.6 GUS ASSAY 29
2.7 LEAF AND CELL SIZE AND CELL NUMBER 29
2.8 ESTRADIOL INDUCTION EXPERIMENT 30
2.9 MICROARRAY ANALYSIS 30
2.10 ETHYLENE TREATMENTS ON SOIL AND IN CULTURE 30
2.11 GLUCOSE, SUCROSE AND MANNITOL RESPONSE EXPERIMENT 30
2.12 IN-VITRO EXPRESSION AND ELECTROPHORETIC-MOBILITY SHIFT ASSAY (EMSA)
31
2.13 DAB STAINING 32
2.14 CHROMATIN IMMUNOPRECIPITATION-QUANTITATIVE PCR 32
2.15 CELL WALL MEASUREMENT 32
2.16 SHAM TREATMENT 32
2.17 CROSSING PLANTS 32
2.18 CONFOCAL MICROSCOPY 33
CHAPTER 3. TRANSCRIPTIONAL REGULATION O F ROS BY KUODA2 CONTROLS LEAF
ORGAN SIZE IN ARABIDOPSIS..34
3.1 ABSTRACT 34
3.2 INTRODUCTION 34
3.3 RESULTS 35
3.3.1 KUA2 IS A POSITIVE REGULATOR O F LEAF GROWTH 35
3.3.2 KUA2 REGULATES FINAL ORGAN SIZE THROUGH CELL EXPANSION BUT NOT
PROLIFERATION 37
3.3.3 KUA2 IS EXPRESSED THROUGHOUT PLANT GROWTH AND DEVELOPMENT 38
3.3.4 KUA2 LOCALIZES TO THE NUCLEUS INPLANTA 39
3.3.5 IDENTIFICATION O F KUA2 TARGET GENES B Y KUA2 INDUCIBLE
OVEREXPRESSION 39
3.3.6 CONFIRMATION O F POTENTIAL KUA2 TARGET GENES B Y QRT-PCR 42
3.3.7 MOTIF ANALYSIS 43
3.3.8 KUA2 BINDS IN VIVO THE ATCACA MOTIF PRESENT IN PROMOTERS O F
TARGET GENES INCLUDING PEROXIDASES 44
3.3.9 SHAM TREATMENT REVERTS KUCI2 PHENOTYPE 45
5
IMAGE 3
3.3.10 CELL WALL COMPOSITION 46
3.3.11 ROS STAINING 46
3.4 CONCLUSIONS 47
CHAPTER 4. ARABIDOPSIS KUA1 IS A KEY TRANSCRIPTIONAL REGULATOR REQUIRED
FOR CELL EXPANSION DURING LEAF GROWTH 48
4.1 ABSTRACT 48
4.2 INTRODUCTION 48
4.3 RESULTS 49
4.3.1 KUA1 IS HIGHLY EXPRESSED IN EXPANDING LEAVES AND FLOWERS 49
4.3.2 KUA1 INFLUENCES MULTIPLE STAGES O F PLANT GROWTH AND DEVELOPMENT
50
4.3.3 KUA1 REGULATES ORGAN SIZE THROUGH CELL EXPANSION 51
4.3.4 PHYLOGENETIC ANALYSIS O F KUA1 52
4.3.5 NUCLEAR LOCALIZATION O F KUA1 IS AFFECTED BY PHOSPHORYLATION 53
4.3.6 KUA1 CONTROLS A SUITE O F ROS-RELATED AND DEVELOPMENTAL GENES 54
4.3.7 CONFIRMATION O F MICROARRAY DATA 58
4.3.8 KUA1 BINDS TO THE PROMOTER O F TARGET GENES IN VITRO 59
4.3.9 TFS INDUCED BY KUA1 ARE ROS-RESPONSIVE AND DIFFERENTIALLY
EXPRESSED IN CELL WALL MUTANTS 60
4.4 CONCLUSIONS 61
CHAPTER 5. KUA1 AND KUA2 REGULATE CELL EXPANSION INTERDEPENDENTLY 63
5.1 ABSTRACT 63
5.2 INTRODUCTION 63
5.3 RESULTS 64
5.3.1 KUA1 AND KUA2 ACT IN PART SYNERGISTICALLY TO CONTROL PLANT ORGAN
SIZE 64
5.3.2 DOUBLE MUTANTS ONLY AFFECT CELL EXPANSION BUT NOT PROLIFERATION 65
5.3.3 DOUBLE MUTANT KUALKUA2 IS HYPERSENSITIVE TO THE INHIBITORY EFFECT
O F ETHYLENE ON CELL EXPANSION 66
5.3.4 DOUBLE MUTANT KUALKUA2 REDUCES HYPOCOTYL LENGTH AND AFFECTS THE
ETHYLENE INDUCED TRIPLE RESPONSE 67
5.3.5 KUA GENES ARE GLUCOSE RESPONSIVE BUT THEIR EXPRESSION IS HXK1
INDEPENDENT 68
6
IMAGE 4
5.3.6 KUA GENES AFFECT SEEDLING GROWTH AND GERMINATION UNDER ELEVATED
LEVELS O F GLUCOSE .69
5.3.7 KUA1 AND KUA2 REGULATE OVERLAPPING GENOMIC TARGETS INVOLVED IN
OXIDATIVE STRESS METABOLISM AND CELL WALL COMPOSITION 69
5.4 CONCLUSION 71
CHAPTER 6. GENERAL DISCUSSION AND OUTLOOK 73
6.1 ALTERED EXPRESSION O F KUODA GENES AFFECTS LEAF ORGAN SIZE IN
ARABIDOPSIS 73
6.2 CELL EXPANSION IS REGULATED BY KUODA GENES 74
6.3 KUODA1 AND KUODA2 GENE REGULATORY NETWORKS ENCOMPASS ROS-RELATED
TRANSCRIPTS AND IN PART OVERLAP 74
6.4 ROLE O F ROS DURING LEAF GROWTH 75
6.5 REGULATION O F ETHYLENE AND GLUCOSE RESPONSES 76
6.6 PROPOSED MODEL FOR KUODA FUNCTION 76
CHAPTER 7. REFERENCES 79
SUPPLEMENTARY MATERIAL 93
ZUSAMMENFASSUNG 120
7
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any_adam_object | 1 |
author | Lu, Dandan |
author_facet | Lu, Dandan |
author_role | aut |
author_sort | Lu, Dandan |
author_variant | d l dl |
building | Verbundindex |
bvnumber | BV040940404 |
ctrlnum | (OCoLC)844057634 (DE-599)GBV739947958 |
dewey-full | 575.5738492364 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 575 - Specific parts of & systems in plants |
dewey-raw | 575.5738492364 |
dewey-search | 575.5738492364 |
dewey-sort | 3575.5738492364 |
dewey-tens | 570 - Biology |
discipline | Biologie |
format | Thesis Book |
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spelling | Lu, Dandan Verfasser aut ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana von Dandan Lu 2012 121 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Potsdam, Univ., Diss., 2012 Transkriptionsfaktor (DE-588)4303350-7 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf Pflanzenwachstum (DE-588)4045595-6 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Pflanzenwachstum (DE-588)4045595-6 s Transkriptionsfaktor (DE-588)4303350-7 s Genregulation (DE-588)4122166-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025919175&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Lu, Dandan ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana Transkriptionsfaktor (DE-588)4303350-7 gnd Ackerschmalwand (DE-588)4141299-0 gnd Pflanzenwachstum (DE-588)4045595-6 gnd Genregulation (DE-588)4122166-7 gnd |
subject_GND | (DE-588)4303350-7 (DE-588)4141299-0 (DE-588)4045595-6 (DE-588)4122166-7 (DE-588)4113937-9 |
title | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana |
title_auth | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana |
title_exact_search | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana |
title_full | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana von Dandan Lu |
title_fullStr | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana von Dandan Lu |
title_full_unstemmed | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana von Dandan Lu |
title_short | ROS transcriptional networks controlling cell expansion during leaf growth in Arabidopsis thaliana |
title_sort | ros transcriptional networks controlling cell expansion during leaf growth in arabidopsis thaliana |
topic | Transkriptionsfaktor (DE-588)4303350-7 gnd Ackerschmalwand (DE-588)4141299-0 gnd Pflanzenwachstum (DE-588)4045595-6 gnd Genregulation (DE-588)4122166-7 gnd |
topic_facet | Transkriptionsfaktor Ackerschmalwand Pflanzenwachstum Genregulation Hochschulschrift |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=025919175&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
work_keys_str_mv | AT ludandan rostranscriptionalnetworkscontrollingcellexpansionduringleafgrowthinarabidopsisthaliana |